Molecular analysis of leptospires from serogroup Sejroe obtained from asymptomatic cattle in Rio de Janeiro — Brazil reveals genetic proximity to serovar Guaricura

Abstract

Bovine leptospirosis causes substantial reproductive failure in cattle, mainly due to infections with serovar (sv) Hardjo infection. Notwithstanding, other serovars from the serogroup (sg) Sejroe could also have important roles in bovine leptospirosis. The objective was to investigate genetic diversity of serogroup Sejroe isolates obtained from asymptomatic cattle in the state of Rio de Janeiro, Brazil. Urine and vaginal fluid (VF) were collected from clinically healthy cattle immediately after slaughter. Five isolates were recovered and characterized (serogrouping) as belonging to sg Sejroe. Sequencing of rrs and secY genes further identified them as Leptospira santarosai. Analysis of secY sequences indicated a high level of sequence homology to sv Guaricura strains. Based on culture and sequence data, we inferred that other members of sg Sejroe may be important in bovine leptospiral infection, particularly genotypes of L. santarosai serovar Guaricura.

VNTR analysis demonstrates new patterns and high genetic diversity of Leptospira sp. of animal origin in Brazil

“…; Loureiro et al . ), one dog (non‐published data), one ewe (Director et al . ), two goats (Lilenbaum et al .…”

“…) and genotyping of particular genes (Loureiro et al . ). Considering this, molecular epidemiology studies contribute to the understanding of the spreading dynamics of leptospires, especially in livestock.…”

Infectivity and virulence of leptospiral strains of serogroup Sejroe other than Hardjo on experimentally infected hamsters

“…Among the Sejroe strains, Hardjo seems to be the most common serovar in the two genotypes Hardjoprajitno (Leptospira interrogans) and Hardjobovis (Leptospira borgpetersenii) [2,5]. More recently, another serovar of the same serogroup, named Guaricura (Leptospira santarosai), has emerged as a major agent of bovine leptospirosis in the Americas [6].…”

“…Leptospira strains (n = 12) were isolated from cattle (10), sheep (1), and buffalo (1) from 1967 to 2016 in Brazil. The used strains were classified through the sequencing of rrs and secY genes and by serogrouping (MAT) using specific polyclonal antisera [6,9]. The descriptions of the Variable Number Tandem Repeat (VNTR) patterns of the inoculated strains were characterized [10].…”

<b>Pesquisa de <i>Leptospira</i> spp. em fêmeas bovinas pertencentes ao município de Novo Repartimento - Pará</b>

“…No presente trabalho houve a detecção do DNA da bactéria em amostras de rim e/ou urina de algumas fêmeas bovinas não reagentes na SAM. A associação da sorologia com a PCR pode ser muito útil na identificação de animais portadores de leptospiras, que usualmente, eliminam a bactéria na urina por longos períodos, contaminando o ambiente e outros animais do rebanho (LILENBAUM, 1996;LILENBAUM et al, 2009 (VICTORIA et al, 2008;PEREZ;GOARANT, 2010;FERREIRA et al, 2014;HAMOND et al, 2015a;HAMOND et al, 2015b;LOUREIRO et al, 2016;MIOTTO et al, 2016).…”