Molecular Analysis of Plasmids in Epidemiologic Investigation

We, Farrar

doi:10.1093/infdis/148.1.1

Cited by 77 publications

(39 citation statements)

References 28 publications

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“…Molecular studies showed that the gentamicin resistance determinant was located on a transposon that had jumped into various plasmids in K. pneumoniae. Many other examples have been reported (47,58,110,111,144,167,195), including those occurring in Staphylococcus aureus (115,127).…”

Section: History Of Plasmid Profiles In Three Examplesmentioning

confidence: 97%

Use of plasmid profiles in epidemiologic surveillance of disease outbreaks and in tracing the transmission of antibiotic resistance

Mayer

1988

Clin Microbiol Rev

152

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Plasmids are circular deoxyribonucleic acid molecules that exist in bacteria, usually independent of the chromosome. The study of plasmids is important to medical microbiology because plasmids can encode genes for antibiotic resistance or virulence factors. Plasmids can also serve as markers of various bacterial strains when a typing system referred to as plasmid profiling, or plasmid fingerprinting is used. In these methods partially purified plasma deoxyribonucleic acid species are separated according to molecular size by agarose gel electrophoresis. In a second procedure, plasmid deoxyribonucleic acid which has been cleaved by restriction endonucleases can be separated by agarose gel electrophoresis and the resulting pattern of fragments can be used to verify the identity of bacterial isolates. Because many species of bacteria contain plasmids, plasmid profile typing has been used to investigate outbreaks of many bacterial diseases and to trace inter- and intra-species spread of antibiotic resistance.

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Section: History Of Plasmid Profiles In Three Examplesmentioning

confidence: 97%

Use of plasmid profiles in epidemiologic surveillance of disease outbreaks and in tracing the transmission of antibiotic resistance

Mayer

1988

Clin Microbiol Rev

152

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“…Colombo and others of pathogenic bacteria based on plasmid content and polymorphisms (e.g. Brunner et al, 1983;Farrar, 1983;Sorum et al, 1988).…”

Section: Introductionmentioning

confidence: 99%

Phenotypic features and molecular characterization of plasmids in Salmonella abortusovis

Colombo

Leori

Rubino

et al. 1992

Journal of General Microbiology

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The phenotypic and genotypic characteristics of 69 wild-type strains of Sulmnella abortusovis from Sardinia and other Italian regions, representing four different epidemic outbreaks, were studied. Biochemical profiles, pathogenicity factors, resistance to antibacterial drugs and genomic organization were investigated. None of the strains tested showed any haemagglutination with different types of red blood cells, and electron microscopy revealed no fimbriae. Adhesivity to epithelial cells was present in all strains tested. Only 16% of the strains were resistant to streptomycin, and no other drug resistances were found. The restriction patterns of chromosomal DNA did not show heterogeneity and a high-molecular-mass (50-67 kb) plasmid was found in all strains. Restriction analysis of plasmid DNA from strains from different geographical areas, collected over a period of 30 years, showed a Hind111 restriction profile characterized by two patterns, one with three fragments, stable and common to all strains, and a second polymorphic pattern with five fragments. The stable pattern included a 3.7 kb Hind111 fragment that hybridized with a S. typhimurium probe containing a virulence region including the spvC and spvD genes. These studies allowed us to outline a genetic correlation among S. abortusovis isolated from different outbreaks.

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“…The search for the plasmid content of strains was often used in the study of epidemiological markers [8,29,30 (4), hessarek, typhimurium (6) the advantage of being quick, reliable and easy to use, in comparison with conventional techniques. The standard methods used to investigate the relationship between the presence of a plasmid and increased virulence call for preliminary extraction of the plasmids followed by the study, in mice, of the effect of strains with or without their plasmids, or the use of DNA/DNA hybridization techniques [7,9,10,11,13,16,21,26].…”

Section: Discussionmentioning

confidence: 99%

Polymerase chain reaction for salmonella virulence–associated plasmid genes detection: a new tool in salmonella epidemiology

Rexach¹,

Dilasser²,

Fach³

1994

Epidemiol. Infect.

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SUMMARYThe important role of plasmid genes in assessing virulence for BALB/c mice in salmonella, and the difficulty of using standard techniques to detect them, led us to develop a detection method by gene amplification.One hundred and forty-three strains (71 serovars) of salmonella and 35 strains of other species were tested using specific oligonucleotide primers. The amplification products were identified by a specific oligonucleotide probe. Forty-nine salmonella strains from ten serovars (S. abortus ovis, S. choleraesuis, S. dublin, S. enteritidis, S. gallinarum/pullorum, S. hessarek, S. typhimurium, S. IIIa 48: Z4, Z23, S. IV43:z4, Z23: -, S. V 28: a: -) produced a positive and specific response.Because of various origins of the strains possessing the gene sought and the diversity of the responses, both from one serovar to another and in the same serovar, this search has its place among the epidemiological markers in general use. This method appears well suited to the research and detection of plasmid genes associated with mouse virulence in salmonella.

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Molecular Analysis of Plasmids in Epidemiologic Investigation

Cited by 77 publications

References 28 publications

Use of plasmid profiles in epidemiologic surveillance of disease outbreaks and in tracing the transmission of antibiotic resistance

Use of plasmid profiles in epidemiologic surveillance of disease outbreaks and in tracing the transmission of antibiotic resistance

Phenotypic features and molecular characterization of plasmids in Salmonella abortusovis

Polymerase chain reaction for salmonella virulence–associated plasmid genes detection: a new tool in salmonella epidemiology

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