Molecular analysis of rice dwarf phytoreovirus segment S11 corresponding to wound tumour phytoreovirus segment S12

In vitro translation of rice dwarf phytoreovirus genome segments S4 to S10

“…Fluorogram of SDS-PAGE of translation products in wheatgerm extracts programmed by synthetic transcripts generated from full-length cDNA clones of RDV genome segments, cDNA clones used for in vitro transcription were: 1 pRS4; 2 pRS5; 3 pRS6; 4 pRS7; 5 pRS8; 6 pRS9; 7 pRS10; 8 pRS11M; 9 pRS12. The products of S11 (P1 la, 8) and S12 (P12, 9) were reported previously [17,19] Concerning the assignment of the S9-coded product, a 45 k outer capsid protein has been proposed [9]. On the other hand, Uyeda et al [23] and Fukumoto et al E3] have suggested that $9 encodes a non-structural protein.…”

“…Of these 12 genome segments, 11 have already been sequenced [3,10,12,13,17,[19][20][21][22][23][24], and only the sequence of the second largest segment $2 has yet to be determined. These sequence analyses have shown that the RDV segments each have a single large open reading frame (ORF), except for $12 [19].…”

“…The data summarized in Table i should provide a basis for a Data are from [9,[17][18][19][20][21][22] and this study for RDV and from [11] for WTV b Calculated from the nucleotide sequence c Calculated from SDS-PAGE data detection and purification of the viral proteins, which are essential for investigation of their functions.…”

“…A cDNA (p9RD3) for the 5'-terminal portion of $9 (base 1-96) was synthesized by the Clamp R method, and then used to obtain full-length cDNA constructs along with p9RD2 (bases 97-902) and p9RD 1 (bases 903-1,305). p9RD 1 and p9RD2 were newly selected from the cDNA library constructed previously [17]. Oligonucleotide primers used for ClampR and PCR are listed in Fig.…”

Nucleotide sequence of Nilaparvata lugens reovirus genome segment S8 coding for the major outer capsid protein

“…However, the AUG existing within 10 bases from the 5' end of mRNA is not well-recognized (Kozak, 1987). Inframe AUGs in the Y-terminal region are also observed in S 11 of rice dwarf virus (RDV; Suzuki et al, 1991).…”