Molecular analysis of Salmonella enteritidis isolates from the Caribbean by pulsed-field gel electrophoresis

Abstract

Using pulsed-field gel electrophoresis (PFGE), between 1987 and 1996 we analyzed Salmonella enteritidis isolates from gastroenteritis cases in four Caribbean countries: Barbados, Saint Kitts and Nevis, Saint Lucia, and Trinidad and Tobago. We also determined the resistance of the isolates to 12 antimicrobial agents. Of the 129 isolates of S. enteritidis available for testing, DNA digested by XbaI revealed 13 distinctive PFGE patterns. The most prevalent XbaI PFGE patterns were group 1 (88 of 129 isolates, 68.2%) and group 2 (26 of 129, 20.2%). The patterns found among S. enteritidis isolates correlated with the geographical origin of the isolates. Of the 28 isolates from Barbados, 20 of them (71.4%) belonged to XbaI PFGE group 2, and of the 93 isolates from Trinidad and Tobago, 78 of them (83.9%) belonged to group 1. SpeI digestion of S. enteritidis genome was not as discriminatory as XbaI. Overall, of the 129 isolates, 67 of them (51.9%) exhibited resistance to one or more of the 12 antimicrobial agents that we tested. The prevalence of resistance was 53.8% for the S. enteritidis isolates tested from Trinidad and Tobago, 50.0% for those from Barbados, 28.6% for those from Saint Lucia, and 100.0% for one isolate from the island of Saint Kitts. Resistance was highest to triple sulfur (59 of 129 isolates, 45.7%), followed by furadantoin (10 of 129, 7.8%), ampicillin (7 of 129, 5.4%), and carbamycin (5 of 129, 3.9%).

Survey of Salmonella Contamination in Chicken Layer Farms in Three Caribbean Countries

“…Adesiyun et al (1) used pulse-field gel electrophoresis to determine the relationships among Salmonella Enteritidis isolates recovered from each of the Caribbean countries studied. Salmonella serotypes differ among layer farms and countries (9,28,29,34), and all serotypes recovered in the present study have been documented previously in the Caribbean region (1,10,20).…”

Antimicrobial resistance of Salmonella spp. and Escherichia coli isolated from table eggs

“…It has been documented that a combination of phage typing, pulse-Weld gel electrophoresis (PFGE) and plasmid proWling considerably increases ability to diVerentiate S. Enteritidis isolates (Cardinale et al, 2005;Cieslik, Browm, Paciorek, Szych, & Kaluzewski, 2001;Lukinmaa, Schildt, Rinttila, & Siitonen, 1999). An earlier study of S. Enteritidis from the Caribbean region employed PFGE to diVerentiate isolates from various countries in the region (Adesiyun, Carson, McAdoo, & Bailey, 2001). In that study, the PFGE patterns of S. Enteritidis strains from Trinidad and Tobago and Barbados were distinctly diVerent.…”

Epidemiological analysis of Salmonella enterica ssp. enterica serovars Hadar, Brancaster and Enteritidis from humans and broiler chickens in Senegal using pulsed-field gel electrophoresis and antibiotic susceptibility

“…The association between poultry and poultry products and S. Enteritidis infections in humans has long been known (Hefferman 1991; Bangtrakulnonth et al. 1994; Adesiyun et al. 2000); in Senegal, S. Enteritidis is the serovar recovered most frequently from cases of human diarrhoea (Pasteur Institute of Dakar, unpublished data) and is also commonly isolated from poultry (Cardinale et al.…”