Molecular analysis of six segments of Tobacco leaf enation virus, a novel phytoreovirus from tobacco

Picton, Anabela C.P.; Potgieter, Christiaan; Rey, M. E. C.

doi:10.1007/s11262-007-0088-x

Cited by 8 publications

(4 citation statements)

References 40 publications

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“…Proteins associated with all 12 segments of the RDV genome can be detected in both insect vector cells and rice, with much higher amounts of protein being present in the plant (163, 164). RGDV, WTV, and Tobacco leaf enation virus (TLEV) have a genomic organization similar to that of RDV, but individual genome segments and encoded proteins have low sequence identity (117,136,198). Segments 1, 2, 3, 5, 7, 8, and 9 encode structural proteins.…”

Section: Reovirusesmentioning

confidence: 99%

Insect Vector Interactions with Persistently Transmitted Viruses

Hogenhout

Ammar

Whitfield

et al. 2008

Annu. Rev. Phytopathol.

951

877

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The majority of described plant viruses are transmitted by insects of the Hemipteroid assemblage that includes aphids, whiteflies, leafhoppers, planthoppers, and thrips. In this review we highlight progress made in research on vector interactions of the more than 200 plant viruses that are transmitted by hemipteroid insects beginning a few hours or days after acquisition and for up to the life of the insect, i.e., in a persistent-circulative or persistent-propagative mode. These plant viruses move through the insect vector, from the gut lumen into the hemolymph or other tissues and finally into the salivary glands, from which these viruses are introduced back into the plant host during insect feeding. The movement and/or replication of the viruses in the insect vectors require specific interactions between virus and vector components. Recent investigations have resulted in a better understanding of the replication sites and tissue tropism of several plant viruses that propagate in insect vectors. Furthermore, virus and insect proteins involved in overcoming transmission barriers in the vector have been identified for some virus-vector combinations.

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Section: Reovirusesmentioning

confidence: 99%

Insect Vector Interactions with Persistently Transmitted Viruses

Hogenhout

Ammar

Whitfield

et al. 2008

Annu. Rev. Phytopathol.

951

877

View full text Add to dashboard Cite

show abstract

“…To further establish the nature of TLEV-associated disease phenotype in tobacco, molecular characterisation of six dsRNA components was undertaken. 49 Results of this study revealed the conserved terminal sequence: 5'GG(U/C)...UGAU 3' of segments S6-S12, while adjacent to these conserved terminal sequences are imperfect inverted repeats (7)(8)(9)(10)(11)(12)(13)(14)(15)…”

Section: First Report Characterisation and Phylogenetic Justificatiomentioning

confidence: 83%

The hunt for plant viruses in South Africa: The early days

Rey

2020

S. Afr. J. Sci.

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Plant viruses cause widespread disease in agriculturally important crops, resulting in a reduction in both quality and quantity of produce. The introduction of intensive crop monoculture has resulted in an exponential increase in viral diseases which can cross over from wild indigenous plants. Viral pathogens also can occur in mixed infections, and rapid, sensitive and reliable diagnostic methods are required to identify and characterise the viruses responsible for the field diseases. In comparison to bacterial and fungal diseases, viral diseases are more difficult to diagnose. This review covers a period (1985–2011) in the history of virus discovery in South Africa during which several plant viruses from commercial and smallscale farms were identified and characterised. Interestingly, novel viruses were discovered in three crops, namely guar and cassava grown by small-scale farmers in Mpumalanga, and in commercial tobacco. The implication of these plant diseases is potential yield loss to farmers which can affect their livelihoods, and result in severe economic loss for the food and agriculture industries. Accurate identification of the causal viral agents of these viral diseases is a prerequisite for development of effective management strategies.

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“…Although most plant reoviruses are found in grasses, there is a recent report of Tobacco leaf enation virus , a novel Phytoreovirus causing a disease in tobacco (Pieton et al., 2007). This cassava virus appears to be another novel plant reovirus in a dicotyledonous host.…”

Section: Discussionmentioning

confidence: 99%

Identification of Three Strains of a Virus Associated with Cassava Plants Affected by Frogskin Disease

Calvert¹,

Cuervo²,

Lozano³

et al. 2008

Journal of Phytopathology

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Cassava Frogskin Disease (CFSD) can cause severe damage to cassava roots and is one of the most important diseases of cassava in Latin America. The principal objective of this study was to identify the causal agent of CFSD. Electron microscopy, viral purifications, double-stranded RNA (dsRNA) analysis, cloning, sequencing, rtPCR and hybridizations were carried out to characterize and associate a novel virus with the disease. Virus-like particles of 70 and 45 nm in diameter were found in affected cassava plants and partially purified preparations respectively. Nine species of dsRNA were associated with this disease and cDNA clones to six genomic segments were synthesized from the purified dsRNAs. The putative proteins predicted from the sequence of the cassava virus cDNA clones have similarity with the P1, P2, P3, P4, P5 and P10 proteins of Rice ragged stunt virus (RRSV). Phylogenic analysis confirmed that this virus is a member of the family Reoviridae and is most closed related to RRSV. Hybridization analyses of dsRNA identified S1, S2, S3, S4, S5 and S10 genomic segments in the CFSD-affected plants, but not in healthy controls. Additionally, 26 isolates were compared using a portion of the putative polymerase gene. The virus was detected in all 26 isolates, and they were classified into three distinct races. The association of this virus with CFSD was strengthened by the detection of this virus in diseased plants collected from different locations throughout Colombia.

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Molecular analysis of six segments of Tobacco leaf enation virus, a novel phytoreovirus from tobacco

Cited by 8 publications

References 40 publications

Insect Vector Interactions with Persistently Transmitted Viruses

Insect Vector Interactions with Persistently Transmitted Viruses

The hunt for plant viruses in South Africa: The early days

Identification of Three Strains of a Virus Associated with Cassava Plants Affected by Frogskin Disease

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