Molecular analysis of the invasive populations of Urochloa (Poaceae) in a large Neotropical reservoir

Diamante, Nathália Alves; Fabrin, Thomaz Mansini Carrenho; Silveira, Márcio José; Oliveira, Alessandra Valéria de; Thomaz, Sidinei Magela; Prioli, Sônia Maria Alves Pinto; Prioli, Alberto José

doi:10.1016/j.aquabot.2019.103183

Cited by 8 publications

(6 citation statements)

References 34 publications

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“…In addition to clonal reproduction, we speculate that the introduction of a small number of individuals may also help to explain the low genetic variability found within a given population. This process acts as a genetic bottleneck, limiting the genetic variation in invasive populations compared to populations from natural sites (Estoup et al 2016;Diamante et al 2020). In the case of specimens of Urochloa of this study, most of them were sampled in the upper Paraná River basin, where they could disperse by river ow, being found as far as the Itaipu Reservoir, where they share the same haplotype, although some sampling points within this reservoir were located more than 80 km apart (Diamante et al 2020).…”

Section: Discussionmentioning

confidence: 99%

“…The correct identi cation of invasive aquatic plant species and information on their genetic variability are fundamental to understanding the mechanism of invasion (Naugzemyz et al 2021) and implementing management strategies, as well as research into potential control agents, including biological ones (Pitelli et al 2008). Molecular tools have been useful for the correct identi cation and analysis of genetic variability in macrophyte populations, including Urochloa in the Upper Paraná River basin (Machado et al 2016;Lucio et al 2019;Diamante et al 2020) and in other watersheds (Collevatti et al 2003;Cloutier et al 2005;Azevedo et al 2008). A previous study with U. arrecta in Itaipu reservoir (Brazil) showed a high degree of genetic homogeneity among populations in this ecosystem (Diamante et al 2020).…”

Section: Introductionmentioning

confidence: 99%

“…Molecular tools have been useful for the correct identi cation and analysis of genetic variability in macrophyte populations, including Urochloa in the Upper Paraná River basin (Machado et al 2016;Lucio et al 2019;Diamante et al 2020) and in other watersheds (Collevatti et al 2003;Cloutier et al 2005;Azevedo et al 2008). A previous study with U. arrecta in Itaipu reservoir (Brazil) showed a high degree of genetic homogeneity among populations in this ecosystem (Diamante et al 2020). However, it remains to be con rmed whether this result applies or not to samples obtained in a variety of ecosystems at a larger spatial scale.…”

Section: Introductionmentioning

confidence: 99%

“…The use of the nuclear genome, speci cally the internal transcribed spacer (ITS) region of nuclear ribosomal DNA, is common for molecular phylogenetic studies of plants (Torres González and Morton, 2005). Moreover, this technique has been used on different taxonomic levels of grasses (Wang et al 2017), in addition to studies concerning molecular discrimination and identi cation (Diamante et al 2020). The use of DNA chloroplast (cpDNA) allows the identi cation of structural and sequence polymorphisms, which may be useful for genetic studies, as well as contributing to phylogenetic Given the potential negative effects of introduced species on native ora and the potential economic consequences of uncontrolled growth of invasive alien species, it is important to understand the processes involved in the occupation and especially of diversi cation of invasive populations.…”

Section: Introductionmentioning

confidence: 99%

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Low genetic diversity in an African invasive Poaceae in Brazil

Scorsim

Diamante

Fabrin

et al. 2022

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Poaceae has numerous species that are highly invasive thorough the planet. Urochloa, native to Africa, stands out in terms of invasion and impacts in South America. However, the correct identification of the species included in this genus is complex due to their morphological similarity with other species and there is a lack of studies involving the genetic variability of some species in Brazil, which is important for understanding the invasion mechanisms and planning control measures. In this study, we evaluated the genetic variability of the invasive exotic Poaceae Urochloa arrecta in different Brazilian regions, by using the nuclear marker ITS and the chloroplast marker trnL-trnF. The sequences obtained were compared with those available in GenBank (NCBI). The results indicated a low genetic differentiation among the sampled populations. Seven and ten distinct haplotypes were identified for ITS and trnL-trnF, respectively, and most specimens shared a single haplotype. These results indicate that a limited number of propagules were introduced in the invasive range and/or that this species reproduces mainly through asexual reproduction. This last possibility is suggested by the great regeneration and colonization rates of asexual propagules of this species. The data obtained are useful for understanding the invasion mechanisms of the group in the region.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Low genetic diversity in an African invasive Poaceae in Brazil

Scorsim

Diamante

Fabrin

et al. 2022

Preprint

Self Cite

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“…(2016) for other species, which shows the dynamism of these sequences and corroborates the interspecific genetic divergence already reported in previous studies. While U. brizantha , U. decumbens , and U. ruziziensis are often grouped and are considered closely related, U. arrecta is allocated in distinct groups, either by morphological classifications (Renvoize et al., 1996) or by molecular analyses (Diamante et al., 2020; Triviño et al., 2017).…”

Section: Discussionmentioning

confidence: 99%

Karyotype analysis and mode of reproduction of two species of Urochloa P. Beauv.

et al. 2021

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Grasses with desirable traits for agriculture and livestock production are continually being sought. The genus Urochloa comprises many species of good forage potential, although studies have mostly focused on commercial cultivars. Karyotype data can be used to characterize species and generate information for taxonomy and evolutionary studies and contribute to breeding programs. The mode of reproduction is also an essential piece of information to determine breeding strategies. This study aimed to characterize the karyotypes of Urochloa arrecta (Hack. ex T. Dur. & Schniz) O. Morrone & F. Zuloaga and a pentaploid cytotype of U. brizantha (Hochst. ex A. Rich.) R. Webster, including the mapping of ribosomal DNA (rDNA) sites and nuclear DNA content and indicating the mode of reproduction. Slides were prepared using the flame drying technique and were subjected to fluorescent in situ hybridization with 35S and 5S rDNA probes. DNA content was estimated by flow cytometry. The classification of the reproduction mode was based on the amplification of the primers N14 and p779/p780. Urochloa arrecta presented 2C = 2.66 pg of DNA, karyotype formula 2n = 4x = 34 m + 2sm, four chromosomes with 35S rDNA sites, and four other chromosomes with 5S rDNA sites. The absence of amplification products with the primers N14 and p779/p780 provided evidence of sexual reproduction. Urochloa brizantha presented 2C = 4.52 pg of DNA and karyotype formula 2n = 5x = 45 m. Five chromosomes presented 35S rDNA, and eight other chromosomes displayed 5S rDNA sites. Polymerase chain reaction results indicated that this cytotype has an apomictic mode of reproduction. The organization of karyotypes suggests an allopolyploid origin for both genotypes. Possible applications of the information found are discussed.

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