Molecular Analysis of the Microbial Community Developing in Continuous Culture of Methylococcus sp. Concept-8 on Natural Gas

Oshkin, Igor Y.; Belova, Svetlana E.; Khokhlachev, N.S.; Semenova, V.A.; Chervyakova, O.P.; Chernushkin, D. V.; Tikhonova, Ekaterina N.; Mardanov, Andrey V.; Равин, Н. В.; Popov, Vladimir O.; Пименов, Н. В.; Dedysh, Svetlana N.

doi:10.1134/s0026261720050173

Cited by 5 publications

(2 citation statements)

References 17 publications

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“…Due to increasing demand on effective technological solutions in the field of production of microbial biomass from natural gas, [40][41][42][43][44][45] the current manuscript provides actual information and applicable decisions valued not only for the general fundamental research in the field but also concerning its applications in developing energy-efficient bioreactors. In order to choose the reasonable experimental setup and analytical tools of the hydrodynamic model, the number of classical approaches 17,18,[46][47][48] as well as recent advanced methods for developing and scaling up industrial biotechnological processes 21,[49][50][51] were studied. Based on the validation results, we got a satisfactory match between the computed and experimental velocity profiles of the fluid flow corresponding to the beginning of the transition to turbulent flow over the flow.…”

Section: Discussionmentioning

confidence: 99%

Simulation of two‐phase air–liquid flows in a closed bioreactor loop: Numerical modeling, experiments, and verification

Nizovtseva

Starodumov

Schelyaev³

et al. 2022

Math Methods in App Sciences

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Improvements in technology for the production of microbial biomass from natural gas, which have been gaining importance in recent years, require the development and optimization of energy‐efficient bioreactor designs. The most advanced methods for developing and scaling up industrial biotechnological processes rely on detailed hydrodynamic modeling, in addition to classical approaches, as the main tool for analysis and large‐scale transfer of experimental information. This paper presents the results of a simulation of flows in a bioreactor circuit using the latest mathematical methods and numerical flow analysis tools for two‐phase bubble flows based on experimental data.

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Section: Discussionmentioning

confidence: 99%

Simulation of two‐phase air–liquid flows in a closed bioreactor loop: Numerical modeling, experiments, and verification

Nizovtseva

Starodumov

Schelyaev³

et al. 2022

Math Methods in App Sciences

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“…Morphological observations and cell size measurements were made with a Axioimager D1 microscope (Zeiss). According to the previously described method, cell morphology was studied with an electron microscope JEM 100CXII (JEOL, Japan) [40]. FS T cells were non-motile, rod-shaped (1.7–3.4 µm long×0.5–0.7 µm wide ().…”

Section: Full-textmentioning

confidence: 99%

Methylocystis silviterrae sp.nov., a high-affinity methanotrophic bacterium isolated from the boreal forest soil

Tikhonova

Grouzdev²,

Avtukh³

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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A novel species is proposed for a high-affinity methanotrophic representative of the genus Methylocystis . Strain FST was isolated from a weakly acidic (pH 5.3) mixed forest soil of the southern Moscow area. Cells of FST are aerobic, Gram-negative, non-motile, curved coccoids or short rods that contain an intracytoplasmic membrane system typical of type-II methanotrophs. Only methane and methanol are used as carbon sources. FST grew at a temperature range of 4–37 °C (optimum 25–30 °C) and a pH range of 4.5 to 7.5 (optimum pH 6.0–6.5). The major fatty acids were C18 : 1ω8c, C18 : 1ω7c and C18 : 0; the major quinone as Q-8. FST displays 16S rRNA gene sequences similarity to other taxonomically recognized members of the genus Methylocystis, with Methylocystis hirsuta CSC1T (99.6 % similarity) and Methylocystis rosea SV97T (99.3 % similarity) as its closest relatives. The genome comprises 3.85 Mbp and has a DNA G+C content of 62.6 mol%. Genomic analyses and DNA–DNA relatedness with genome-sequenced members of the genus Methylocystis demonstrated that FST could be separated from its closest relatives. FST possesses two particulate methane monooxygenases (pMMO): low-affinity pMMO1 and high-affinity pMMO2. In laboratory experiments, it was demonstrated that FST might oxidize methane at atmospheric concentration. The genome contained various genes for nitrogen fixation, polyhydroxybutyrate synthesis, antibiotic resistance and detoxification of arsenic, cyanide and mercury. On the basis of genotypic, phenotypic and chemotaxonomic characteristics, it is proposed that the isolate represents a novel species, Methylocystis silviterrae sp. nov. The type strain is FST (=KCTC 82935T=VKM B-3535T).

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Laboratory scale bioreactor designs in the processes of methane bioconversion: Mini-review

Tikhomirova

But²

2021

Biotechnology Advances

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Molecular Analysis of the Microbial Community Developing in Continuous Culture of Methylococcus sp. Concept-8 on Natural Gas

Cited by 5 publications

References 17 publications

Simulation of two‐phase air–liquid flows in a closed bioreactor loop: Numerical modeling, experiments, and verification

Simulation of two‐phase air–liquid flows in a closed bioreactor loop: Numerical modeling, experiments, and verification

Methylocystis silviterrae sp.nov., a high-affinity methanotrophic bacterium isolated from the boreal forest soil

Laboratory scale bioreactor designs in the processes of methane bioconversion: Mini-review

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