2022
DOI: 10.1093/cid/ciac452
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Molecular Analysis With 16S rRNA PCR/Sanger Sequencing and Molecular Antibiogram Performed on DNA Extracted From Valve Improve Diagnosis and Targeted Therapy of Infective Endocarditis: A Prospective Study

Abstract: Background Molecular analysis (MA) on heart valve (HV) improves the microbiologic diagnosis of infectious endocarditis (IE). The main drawback of MA is the lack of antimicrobial susceptibility information. Methods We conducted a prospective cohort observational study of consecutive adult patients from April 2012 to May 2021 who underwent valve surgery at our hospital. The performance of MA, blood culture (BC) and valve cultur… Show more

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Cited by 12 publications
(7 citation statements)
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“…The molecular anti-biogram was based on genes encoding for multi-drug resistant mechanisms. It showed 100% concordance with anti-microbial susceptibility testing [58].…”
Section: Molecular Techniquesmentioning
confidence: 69%
“…The molecular anti-biogram was based on genes encoding for multi-drug resistant mechanisms. It showed 100% concordance with anti-microbial susceptibility testing [58].…”
Section: Molecular Techniquesmentioning
confidence: 69%
“…In order to assess the diagnostic accuracy of the FISHseq method, we calculated the positive and negative percentage agreement and their 95% confidence intervals (CIs), as previously described [ 24 , 32 ] and in accordance with Food and Drug Administration (FDA) recommendations [ 33 ]. All statistical analyses were performed with R 4.2.0 (R Foundation for Statistical Computing, Vienna, Austria).…”
Section: Methodsmentioning
confidence: 99%
“…A persistent diagnostic challenge is the prevalence of culture negative endocarditis. In many studies, > 30% of patients will have negative blood cultures, complicating their care [21,22]. This is often because patients receive antibiotics before cultures are obtained or due to infection with difficult to culture organisms.…”
Section: Diagnosismentioning
confidence: 99%