Molecular and Biological Characterization of the First Hypovirus Identified in Fusarium oxysporum

Torres-Trenas, Almudena; Cañizares, M. Carmen; García-Pedrajas, Maria D.; Pérez-Artés, Encarnación

doi:10.3389/fmicb.2019.03131

Cited by 18 publications

(17 citation statements)

References 57 publications

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“…Numerous mycoviruses have been described from different families, infecting many phytopathogenic fungi and entomopathogenic fungi [ 2 , 8 , 46 ]. However, among the hundreds of mycoviruses described so far, only six mycoviruses have been identified in F. oxysporum , an important phytopathogenic species [ 34 , 35 , 36 , 37 , 38 , 39 ], with no reports of any viruses belonging to the proposed family “Alternaviridae”. FoAV1 is the first alternavirus described to infect F. oxysporum .…”

Section: Discussionmentioning

confidence: 99%

“…In recent years, a large number of hypovirulent mycoviruses, such as CHV1 [ 13 ] and SsHADV1 [ 49 ], have been identified and explored as potential biocontrol agents against fungal diseases [ 4 , 32 , 33 ]. However, until now, only two mycoviruses, FodV1 and FoOuLV1, isolated from F. oxysporum , exerted hypovirulent effects [ 34 , 35 , 36 , 37 , 38 , 39 ]. FoAV1 exhibited a good biological control effect for Fusarium wilt, suggesting that it may be a good resource for controlling Fusarium wilt.…”

Section: Discussionmentioning

confidence: 99%

“…dianthi mitovirus 1 (FodMV1), classified as family Narnaviridae ; Fusarium oxysporum f. sp. dianthi hypovirus 2 (FodHV2), classified as family Hypoviridae ; Hadaka Virus 1 (HadV1), classified as family Polymycoviridae ; and Fusarium oxysporum ourmia-like virus 1 (FoOuLV1), classified as family Botourmiaviridae [ 34 , 35 , 36 , 37 , 38 , 39 ]. Only FodV1 and FoOuLV1 are known to cause hypovirulence [ 39 , 40 ].…”

Section: Introductionmentioning

confidence: 99%

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Molecular Characterization of the First Alternavirus Identified in Fusarium oxysporum

Wen

Wan

Zhang

et al. 2021

Viruses

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A novel mycovirus named Fusarium oxysporum alternavirus 1(FoAV1) was identified as infecting Fusarium oxysporum strain BH19, which was isolated from a fusarium wilt diseased stem of Lilium brownii. The genome of FoAV1 contains four double-stranded RNA (dsRNA) segments (dsRNA1, dsRNA 2, dsRNA 3 and dsRNA 4, with lengths of 3.3, 2.6, 2.3 and 1.8 kbp, respectively). Additionally, dsRNA1 encodes RNA-dependent RNA polymerase (RdRp), and dsRNA2- dsRNA3- and dsRNA4-encoded hypothetical proteins (ORF2, ORF3 and ORF4), respectively. A homology BLAST search, along with multiple alignments based on RdRp, ORF2 and ORF3 sequences, identified FoAV1 as a novel member of the proposed family “Alternaviridae”. Evolutionary relation analyses indicated that FoAV1 may be related to alternaviruses, thus dividing the family “Alternaviridae” members into four clades. In addition, we determined that dsRNA4 was dispensable for replication and may be a satellite-like RNA of FoAV1—and could perhaps play a role in the evolution of alternaviruses. Our results provided evidence for potential genera establishment within the proposed family “Alternaviridae”. Additionally, FoAV1 exhibited biological control of Fusarium wilt. Our results also laid the foundations for the further study of mycoviruses within the family “Alternaviridae”, and provide a potential agent for the biocontrol of diseases caused by F. oxysporum.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular Characterization of the First Alternavirus Identified in Fusarium oxysporum

Wen

Wan

Zhang

et al. 2021

Viruses

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“…the Sclerotinia sclerotiorum strain Ep-IPN and observed that its attenuating factor (dsRNA) could be transferred to a strain with normal vegetative affinity (Ep-IPNA183) through soil and rape leaves, which caused the virulence of the normal strain to decline and protected the plants from any damage caused by the latter strain (Azhar et al, 2019;Torres-Trenas et al, 2020). However, there are many problems that need to be solved when fungal viruses are applied in actual plant production.…”

Section: Fungal Virusesmentioning

confidence: 99%

Research Progress on Phytopathogenic Fungi and Their Role as Biocontrol Agents

et al. 2021

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Phytopathogenic fungi decrease crop yield and quality and cause huge losses in agricultural production. To prevent the occurrence of crop diseases and insect pests, farmers have to use many synthetic chemical pesticides. The extensive use of these pesticides has resulted in a series of environmental and ecological problems, such as the increase in resistant weed populations, soil compaction, and water pollution, which seriously affect the sustainable development of agriculture. This review discusses the main advances in research on plant-pathogenic fungi in terms of their pathogenic factors such as cell wall-degrading enzymes, toxins, growth regulators, effector proteins, and fungal viruses, as well as their application as biocontrol agents for plant pests, diseases, and weeds. Finally, further studies on plant-pathogenic fungal resources with better biocontrol effects can help find new beneficial microbial resources that can control diseases.

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“…For instance, Cryphonectria parasitica hypovirus 1 (CHV1) has been used to control chestnut blight caused by Cryphonectria parasitica [9,10]. In Fusarium species, more than 30 mycoviruses have been identi ed and fully sequenced [9][10][11][12][13][14]. 4 mycoviruses were associated with hypovirulence of the host Fusarium, and notably, 3 viruses were from F. graminearum [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

FpgMBV1 Alleviates The Pathogenicity of Fusarium Pseudograminearum to Wheat Plants: A Megabirnavirus Case Study

Xie¹,

Wang²,

Li³

et al. 2020

Preprint

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Background: Fusarium pseudograminearum is the predomenant causal agent of devastating crown rot diseases in cereal crops around the world. Mycoviruses have attracted increasing attention as potential biological control agents on plant diseases. The unique mycoviruse isolated from F. pseudograminearum is Fusarium pseudograminearum megabirnavirus 1 (FpgMBV1), which is a new member of the family Megabirnaviridae. To determine the hypovirulence effects of FpgMBV1 on F. pseudograminearum to wheat plants is critical for the potential application of FpgMBV1 in the control of cereal crown rot disease. Methods: Hyphal tip cultures were conducted to obtain a FpgMBV1-free strain, named as FC136-2A-V－. A hyg gene was transformed into a highly virulent virus-negative stain WZ-8A of F. pseudograminearum to obtain the deduced strain WZ-8A-HygR-V－. WZ-8A-HygR-V－ was used in pairing culture with the FpgMBV1-carrying F. pseudograminearum strain FC136-2A to obtain a FpgMBV1-positive strain WZ-8A-HygR-V+. Then the two pairs of strains, FC136-2A-V－/FC136-2A and WZ-8A-HygR-V－/WZ-8A-HygR-V+, were used to determine the potential effect on F. pseudograminearum by the infection of FpgMBV1 through tests on the growth, sensitivity to stress and cellophane penetrating ability in vitro and the pathogenicity to wheat plants.Results: FpgMBV1 could be cured by hyphal tip culture and horizontally transferred between F. pseudograminearum strains through pairing culture. Reduction of FpgMBV1-carrying strains on hyphal growth were found under the treatment of 0.5% SDS. No differences in the growth rates of tested strains in the treatments with 1 M NaCl, 1 M KCl, or 15 mM H2O2. Comparing to virus-negative strains, the In vitro cellophane penetrating ability was lost in FpgMBV1-carrying strains. The disease index of wheat plants inoculated with FC136-2A-V- was significantly higher than that inoculated with FC136-2A, while the pathogenicity of WZ-8A-HygR-V+ reduced significantly comparing to that of WZ-8A-HygR-V－.Conclusions: FpgMBV1 is the causal agent of the low pathogenicity to wheat plants of its original host F. pseudograminearum strain FC136-2A. And FpgMBV1 could be horizontally transferred to another F. pseudograminearum strain and reduce its pathogenicity to wheat plants.

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Molecular and Biological Characterization of the First Hypovirus Identified in Fusarium oxysporum

Cited by 18 publications

References 57 publications

Molecular Characterization of the First Alternavirus Identified in Fusarium oxysporum

Molecular Characterization of the First Alternavirus Identified in Fusarium oxysporum

Research Progress on Phytopathogenic Fungi and Their Role as Biocontrol Agents

FpgMBV1 Alleviates The Pathogenicity of Fusarium Pseudograminearum to Wheat Plants: A Megabirnavirus Case Study

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