Molecular and cellular characterization of expanded and cryopreserved human limbal epithelial stem cells reveal unique immunological properties

Vasania, Viraf S.; Prasad, Parvathy; Gill, Rajdeep Kaur; Mehta, Ashish; Viswanathan, Chandra; Sarang, Shabari; Majumdar, Anish Sen

doi:10.1016/j.exer.2010.11.001

Cited by 17 publications

(9 citation statements)

References 43 publications

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“…19 Human hepatocytes do not tolerate cryopreservation, as reflected by impairment of cell adhesion, morphological changes in mitochondria, loss of ATP production, alteration in mitochondrial respiratory chain enzymes, increased mitochondrial permeability, and loss of membrane potential. 20 Nevertheless, viability test of cryopreserved SG in this study exhibited a good percentage of recovery cells in agreement with recovery of cryopreserved human limbal epithelial stem cells and conjunctival epithelial stem cells (*70%-80%) 16,17 In SGs, personal stem cell banking may be important in the repair process by autologous transplantation after IR for three reasons: transplantationcan only be performed after irradiation treatment, which lasts 2 months. This time frame might result in reduced transplantation efficiency due to a decrease in cell-specific fraction numbers, as has been reported for c-KIT-expressing salivary stem cells.…”

Section: Discussionsupporting

confidence: 77%

“…Stem cell storage and banking are fundamental issues in tissue repair, as it has been suggested for several epithelialoriginated organs such as human limbal epithelial stem cells, 16 conjunctival epithelial stem cells, 17 pancreatic ductal cells, 18 and hepatocytes. 19 Human hepatocytes do not tolerate cryopreservation, as reflected by impairment of cell adhesion, morphological changes in mitochondria, loss of ATP production, alteration in mitochondrial respiratory chain enzymes, increased mitochondrial permeability, and loss of membrane potential.…”

Section: Discussionmentioning

confidence: 99%

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Long-Term Cryopreservation Model of Rat Salivary Gland Stem Cells for Future Therapy in Irradiated Head and Neck Cancer Patients

Neumann

David²,

Stiubea-Cohen³

et al. 2012

Tissue Engineering Part C: Methods

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Irradiated head and neck cancer patients suffer from irreversible loss of salivary gland (SG) function, along with significant morbidity and compromised quality of life. To date there is no biologically-based treatment for this distress. Adult salivary gland stem cells are promising candidates for autologous transplantation therapy in the context of tissue-engineered artificial SGs or direct cell therapy. The major restrictions in handling such cells are their limited lifespan during in vitro cultivation, resulting in a narrow time-window for implantation and a risk of tumorigenic changes during culture. To overcome these difficulties, we tested in a rat model the possibility of establishing a personal/autologous SG stem cell bank. SG's integrin-α6β1-expressing cells were shown to hold a subpopulation of SG-specific progenitor-cells. Explanted and cultured single cell-originated clones were cryopreserved for up to 3 years and shown to exhibit genetic and functional stability similar to noncryopreserved cells, as was emphasized by soft agar assay, division potential assessment, flow cytometric analysis, real-time reverse transcriptase-polymerase chain reaction, in vitro three-dimensional differentiation assay, and immunofluorescence confocal microscopy. Future integration of the novel strategies presented herein to a clinical therapeutic model will allow safe preservation until transplantation and repeated transplantation if needed. These tools open a new venue for adult autologous stem-cell transplantation-based SG regeneration.

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Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Long-Term Cryopreservation Model of Rat Salivary Gland Stem Cells for Future Therapy in Irradiated Head and Neck Cancer Patients

Neumann

David²,

Stiubea-Cohen³

et al. 2012

Tissue Engineering Part C: Methods

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“…Allogeneic LESC cultures have also been used successfully. Interestingly, cryopreserved and expanded LESC cultures lose immunogenicity by downregulating major histocompatibility complex (MHC) protein expression, making immune rejection less of a problem . Historically, LESC were expanded for transplantation using fetal bovine serum in the medium and mouse 3T3 cells as a feeder layer .…”

Section: Stem Cells For Epithelial Wound Healingmentioning

confidence: 99%

Concise Review: Stem Cells for Corneal Wound Healing

et al. 2017

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Corneal wound healing is a complex process that occurs in response to various injuries and commonly used refractive surgery. It is a significant clinical problem, which may lead to serious complications due to either incomplete (epithelial) or excessive (stromal) healing. Epithelial stem cells clearly play a role in this process, whereas the contribution of stromal and endothelial progenitors is less well studied. The available evidence on stem cell participation in corneal wound healing is reviewed, together with the data on the use of corneal and non‐corneal stem cells to facilitate this process in diseased or postsurgical conditions. Important aspects of corneal stem cell generation from alternative cell sources, including pluripotent stem cells, for possible transplantation upon corneal injuries or in disease conditions are also presented. Stem Cells 2017;35:2105–2114

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“…Recent studies suggest that CBT is a safe and effective strategy for adult patients lacking a suitable related or unrelated donor (6). Autologous cell transplantation is not a viable option for diabetic patients with bilateral limbal SC deficiency (7).…”

Section: Introductionmentioning

confidence: 99%

Effect of Stem Cell Therapy on Induced Diabetic Keratopathy in Albino Rat

Zickri¹,

Ahmad²,

Maadawi³

et al. 2012

Int J Stem Cells

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Background and Objectives: Type 2 diabetes mellitus (DM) is a prevalent disorder. Diabetic keratopathy is a well-known ocular complication secondary to type 2 DM. Topical insulin application did not affect apoptosis and necrosis levels in corneal epithelium. Autologous cell transplantation is not a viable option for diabetic patients with bilateral limbal stem cell deficiency. The present study aimed at assessing the possible effect of hemopoeitic stem cell (HSC) therapy on induced diabetic keratopathy in albino rat. Methods and Results: Fifteen male albino rats were divided into control group of 2 rats, diabetic group of 8 rats receiving single intraperitoneal (IP) injection of 50 mg/kg streptozotocin (STZ). 3 animals were sacrificed 6 weeks following confirmation of diabetes to confirm keratopathy and 5 rats were sacrificed 4 weeks following confirmation of keratopathy. SC therapy group included 5 rats injected with HSCs 6 weeks following confirmation of diabetes and sacrificed 4 weeks following SC therapy. Cord blood collection, stem cells isolation and labeling were performed. Eye specimens were subjected to histological, histochemical, immunohistochemical, morphometric and statistical studies. In diabetic group, the central cornea showed multiple cells with vacuolated cytoplasm and dark nuclei, focal epithelial discontinuity, reduced corneal thickness and less number of layers of corneal and conjunctival epithelia. In stem cell therapy group, few cells with vacuolated cytoplasm and dark nuclei were found in the corneal and conjunctival epithelia with more number of epithelial layers. Conclusions: A definite ameliorating effect of HSC therapy was detected on diabetic keratopathy. The therapeutic cells were effective in limiting corneal epithelial changes.

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Molecular and cellular characterization of expanded and cryopreserved human limbal epithelial stem cells reveal unique immunological properties

Cited by 17 publications

References 43 publications

Long-Term Cryopreservation Model of Rat Salivary Gland Stem Cells for Future Therapy in Irradiated Head and Neck Cancer Patients

Long-Term Cryopreservation Model of Rat Salivary Gland Stem Cells for Future Therapy in Irradiated Head and Neck Cancer Patients

Concise Review: Stem Cells for Corneal Wound Healing

Effect of Stem Cell Therapy on Induced Diabetic Keratopathy in Albino Rat

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