Molecular and fibrillar structure collagen analysis by FTIR spectroscopy

Nashchekina, Yu. A.; Starostina, A. A.; Trusova, N. A.; Sirotkina, M. Yu.; Lihachev, Alexey; Нащекин, А. В.

doi:10.1088/1742-6596/1697/1/012053

Cited by 20 publications

(9 citation statements)

References 7 publications

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“…We observed the presence of amide III at 1340 cm −1 , confirming that the intact triple-helical structure of collagen was well maintained [27,30,31]. Previous analyses have reported that peptide bonds in the collagen structure can be damaged by UV exposure [31,32]; however, we did not find changes or absences in the elemental analysis of the composition of the collagen peptide, suggesting that UV irradiation did not impair collagen crosslinking.…”

Section: Resultssupporting

confidence: 77%

Functionalized Collagen/Poly(Ethylene Glycol) Diacrylate Interpenetrating Network Hydrogel Enhances Beta-Pancreatic Cell Sustenance

Moreno-Castellanos¹,

Cuartas-Gómez²,

Vargas-Ceballos³

2023

Preprint

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Three-dimensional matrices are a new strategy used to tackle type I diabetes; a chronic metabolic disease characterized by the destruction of beta pancreatic cells. Type I collagen is an abundant extracellular matrix (ECM), component that has been used to support cell growth. However, pure collagen possesses some difficulties including low stiffness and strength, and high susceptibility to cell-mediated contraction. Therefore, we developed a collagen hydrogel with a poly(ethylene glycol) diacrylate (PEGDA) interpenetrating network (IPN), functionalized with vascular endothelial growth factor (VEGF) to mimic the pancreatic environment for the sustenance of beta-pancreatic cells. We analyzed the physicochemical characteristic of the hydrogels and found that they were successfully synthesized. The mechanical behavior of the hydrogels improved with the addition of VEGF, and the swelling degree and the degradation were stable over time. In addition, it was found that 5 ng/mL VEGF-functionalized collagen/PEGDA IPN hydrogels sustained and enhanced viability, proliferation, respiratory capacity and functionality of beta pancreatic cells. Hence, this is a potential candidate for future preclinical evaluation, which may be favorable for diabetes treatment.

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Section: Resultssupporting

confidence: 77%

Functionalized Collagen/Poly(Ethylene Glycol) Diacrylate Interpenetrating Network Hydrogel Enhances Beta-Pancreatic Cell Sustenance

Moreno-Castellanos¹,

Cuartas-Gómez²,

Vargas-Ceballos³

2023

Preprint

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“…According to previously published data, there is an intensity increase at the amide III peak (1.242 cm −1 ) when collagen transitions from monomeric solution to fibrillar structures [ 35 ]. Earlier, we also confirmed the possibility of using FTIR spectroscopy to analyze the transition of collagen from molecular to fibrillar form [ 36 ]. This peak (1.242 cm −1 ) corresponds to an N-H bending mode that is attributed to the proline side chains in fibrillar collagen [ 37 ].…”

Section: Resultssupporting

confidence: 73%

The Structural Interactions of Molecular and Fibrillar Collagen Type I with Fibronectin and Its Role in the Regulation of Mesenchymal Stem Cell Morphology and Functional Activity

Nashchekina

Nikonov

Prasolov

et al. 2022

IJMS

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The observed differences in the structure of native tissue and tissue formed in vitro cause the loss of functional activity of cells cultured in vitro. The lack of fundamental knowledge about the protein mechanism interactions limits the ability to effectively create in vitro native tissue. Collagen is able to spontaneously assemble into fibrils in vitro, but in vivo, other proteins, for example fibronectin, have a noticeable effect on this process. The molecular or fibrillar structure of collagen plays an equally important role. Therefore, we studied the interaction of the molecular and fibrillar structure of collagen with fibronectin. Atomic force and transmission electron microscopy showed that the presence of fibronectin does not affect the native structure and diameter of collagen fibrils. Confocal microscopy demonstrated that the collagen structure affects the cell morphology. Cells are better spread on molecular collagen compared with cells cultured on fibrillar collagen. Fibronectin promotes the formation of a large number of focal contacts, while in combination with collagen of both forms, its effect is leveled. Thus, understanding the mechanisms of the relationship between the protein structure and composition will effectively manage the creation in vitro of a new tissue with native properties.

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“…Collagen displays bands at 1647, 1547, and 1240 cm −1 , which are characteristic of the amide I, II, and III bands of collagen. The amide I absorption arises predominantly from protein amide C=O stretching vibrations [ 36 ]. The amide II absorption is made up of amide N–H bending vibrations and C–N stretching vibrations.…”

Section: Resultsmentioning

confidence: 99%

Collagen Scaffolds Treated by Hydrogen Peroxide for Cell Cultivation

et al. 2021

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Collagen in the body is exposed to a range of influences, including free radicals, which can lead to a significant change in its structure. Modeling such an effect on collagen fibrils will allow one to get a native structure in vitro, which is important for modern tissue engineering. The aim of this work is to study the effect of free radicals on a solution of hydrogen peroxide with a concentration of 0.006–0.15% on the structure of collagen fibrils in vitro, and the response of cells to such treatment. SEM measurements show a decrease in the diameter of the collagen fibrils with an increase in the concentration of hydrogen peroxide. Such treatment also leads to an increase in the wetting angle of the collagen surface. Fourier transform infrared spectroscopy demonstrates a decrease in the signal with wave number 1084 cm−1 due to the detachment of glucose and galactose linked to hydroxylysine, connected to the collagen molecule through the -C-O-C- group. During the first day of cultivating ASCs, MG-63, and A-431 cells, an increase in cell adhesion on collagen fibrils treated with H2O2 (0.015, 0.03%) was observed. Thus the effect of H2O2 on biologically relevant extracellular matrices for the formation of collagen scaffolds was shown.

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Molecular and fibrillar structure collagen analysis by FTIR spectroscopy

Cited by 20 publications

References 7 publications

Functionalized Collagen/Poly(Ethylene Glycol) Diacrylate Interpenetrating Network Hydrogel Enhances Beta-Pancreatic Cell Sustenance

Functionalized Collagen/Poly(Ethylene Glycol) Diacrylate Interpenetrating Network Hydrogel Enhances Beta-Pancreatic Cell Sustenance

The Structural Interactions of Molecular and Fibrillar Collagen Type I with Fibronectin and Its Role in the Regulation of Mesenchymal Stem Cell Morphology and Functional Activity

Collagen Scaffolds Treated by Hydrogen Peroxide for Cell Cultivation

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