Molecular and functional characterization of IL-6 receptor (IL-6R) and glycoprotein 130 (gp130) in Nile tilapia (Oreochromis niloticus)

Zhou, Enxu; Yan, Fangfang; Li, Bingxi; Chen, Meng; Tu, Xiao; Wu, Siwei; Wu, Haiyan; Wei, Xin; Fu, Shengli; Wu, Liting; Guo, Zheng; Ye, Jianmin

doi:10.1016/j.dci.2020.103629

Cited by 25 publications

(13 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To investigate impact of stimuli on the PfMHC Ⅰα mRNA expressions in vitro, the leucocytes were added to 96‐well microplates (Corning) (1 × 10 6 cells well −1 ) and incubated with inactivated A. hydrophila (Fu, Ding, Liu, et al., 2019), 50 μg/ml Poly I:C (Zhou et al., 2020) and the equal volume sterile 1 × PBS, respectively. The A. hydrophila was inactivated by addition of 40% (W/V) formalin to the broth culture at a final concentration of 0.5% (V/V) and left at room temperature for 48 hr.…”

Section: Methodsmentioning

confidence: 99%

Molecular cloning, characterization and expression analysis of major histocompatibility complex class I alpha gene of pufferfish (Takifugu obscurus)

Liu

Huang

et al. 2020

Journal of Fish Diseases

Self Cite

View full text Add to dashboard Cite

The major histocompatibility complex (MHC) genes play a key role in immune response in vertebrates. In this study, an MHC I alpha homolog gene (PfMHC Ⅰα) from pufferfish (Takifugu obscurus) was identified and its subcellular localization and expression patterns of PfMHC Ⅰα after challenge in vivo and in vitro were analysed. The open reading frame of PfMHC Ⅰα was 1,089 bp in length, encoding 362 aa. The immunofluorescence result revealed that PfMHC Ⅰα was presented on the membrane of lymphocytes. qRT‐PCR analysis indicated that PfMHC Ⅰα was expressed in all examined tissues, with the highest expression in skin, followed by the expression in gills and whole blood. After challenge of Aeromonas hydrophila or polyinosinic: polycytidylic acid (Poly I:C) in vitro, the expression levels of PfMHC Ⅰα on pufferfish kidney lymphocytes were significantly up‐regulated, with the highest expression level at 48 hr post‐challenge. After infection with A. hydrophila or Poly I:C in vivo, the expression levels of PfMHC Ⅰα in the skin, whole blood and kidneys were significantly up‐regulated. Taken together, it is speculated that PfMHC Ⅰα associates with resistance to both intracellular and extracellular antigens and plays an important role in the host response against pathogen infection in pufferfish.

show abstract

Section: Methodsmentioning

confidence: 99%

Molecular cloning, characterization and expression analysis of major histocompatibility complex class I alpha gene of pufferfish (Takifugu obscurus)

Liu

Huang

et al. 2020

Journal of Fish Diseases

Self Cite

View full text Add to dashboard Cite

show abstract

“…IL-6 is involved in brain development including cognitive processes of memory formation and cognitive flexibility by promoting differentiation and proliferation of neurons (5,12). IL-6 is also involved in hematopoiesis, metabolism (13) and post-prandial glycemia improvement. Through delayed gastric emptying it regulates appetite and is such involved in body weight control, as a result of hypothalamic neuropeptide regulation (14).…”

Section: Interleukin-6mentioning

confidence: 99%

“…It is involved in humoral responses against influenza virus post-vaccination, registers high levels in advanced human immunodeficiency virus (HIV) infection, has antibacterial properties by inhibiting gram-negative bacteria and is involved in fighting parasitic infections such as filaria (31)(32)(33)(34)(35)(36)(37). It is involved in nervousness (13), osteoporosis (as a protective agent by promoting osteogenesis through angiogenesis) (4), cognitive dysfunction in neurodegenerative diseases (Huntington's, Parkinson's and Alzheimer's diseases, multiple sclerosis) and psychiatric disorders (major depression, schizophrenia, bipolar disorder) (12,38). IL-6 is involved in metabolic syndrome including obesity and type 2 diabetes (11,38), coagulation disorders (39), neuropathic pain, autoimmune disorders and ischemic injury (18).…”

Section: Interleukin-6mentioning

confidence: 99%

“…The multifunctional activity of IL-6 is mediated by its cell surface type-I receptor complex consisting of two components-the α-receptor subunit (CD126 or IL-6R), a type I transmembrane glycoprotein weighing 80 kDa, and the signal-transducing β-receptor subunit (CD130 or gp130), a type I transmembrane protein of 130 kDa (13).…”

Section: Interleukin-6mentioning

confidence: 99%

“…Cluster signaling, a newly discovered type of signaling model, is a complex process described during the interaction between T helper17 (Th17) and dendritic cells. The IL-6/mIL-6R complex, found on the transmitter cells, sets off the activation of mgp130 on the receiver cells (13).…”

Section: Interleukin-6mentioning

confidence: 99%

See 2 more Smart Citations

Multifactorial expression of IL‑6 with update on COVID‑19 and the therapeutic strategies of its blockade (Review)

et al. 2021

View full text Add to dashboard Cite

Interleukin 6 (IL-6), a cytokine produced by various cells of the human body (macrophages, lymphocytes, astrocytes, ischemic myocytes, endothelial cells) has both pro-inflammatory and anti-inflammatory properties, being a key component in regulating various physiologic and pathological processes. The structure of this molecule and the receptor system it possesses are important due to the different activities that IL-6 can exert; through trans-signaling pro-inflammatory activities are mediated, while through classic signaling, IL-6 is responsible for anti-inflammatory and regenerative activities. IL-6 signaling is involved in coronary artery disease and the global COVID-19 pandemic. This proatherogenic cytokine reaches elevated serum levels in the cytokine storm generated by SARS-CoV-2, and is also associated with smoking or obesity-classic cardiovascular risk factors which promote inflammatory states. IL-6 levels are proportionally correlated with dyslipidemia, hypertension and glucose dysregulation, and they are associated with poor outcomes in patients with unstable angina or acute myocardial infarction. IL-6 targeting for treatment development (not only) in cardiovascular disease and COVID-19 is still a matter of ongoing research, although tocilizumab has proven to be effective in reducing the proatherogenic effects of IL-6 and is suggested to improve COVID-19 patient survival.

show abstract

Expression and functional characterization of chemokine CXCL10 from Nile tilapia (Oreochromis niloticus) in response to bacterial infection and C1q-mediated regulation of monocytes/macrophages activity

Chen

Yan

Wei

et al. 2021

Aquaculture Reports

View full text Add to dashboard Cite

Molecular and functional characterization of IL-6 receptor (IL-6R) and glycoprotein 130 (gp130) in Nile tilapia (Oreochromis niloticus)

Cited by 25 publications

References 66 publications

Molecular cloning, characterization and expression analysis of major histocompatibility complex class I alpha gene of pufferfish (Takifugu obscurus)

Molecular cloning, characterization and expression analysis of major histocompatibility complex class I alpha gene of pufferfish (Takifugu obscurus)

Multifactorial expression of IL‑6 with update on COVID‑19 and the therapeutic strategies of its blockade (Review)

Expression and functional characterization of chemokine CXCL10 from Nile tilapia (Oreochromis niloticus) in response to bacterial infection and C1q-mediated regulation of monocytes/macrophages activity

Contact Info

Product

Resources

About