Molecular and genetic analysis of the transferred DNA regions of the root-inducing plasmid of Agrobacterium rhizogenes

White, Frank F.; Taylor, Brian H.; Huffman, Gary; Gordon, Milton P.; Nester, Eugene W.

doi:10.1128/jb.164.1.33-44.1985

Cited by 478 publications

(92 citation statements)

References 44 publications

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“…Cultivation of HRCs on standard medium results in low FAZY expression and absence of fructan accumulation Seven independent chicory HRC lines were established using Agrobacterium rhizogenes strain R1000 carrying the pRiA-4b plasmid (White et al, 1985) for transforming chicory seedlings. HRC lines were efficiently propagated on solid standard Gamborg B5 (SM) medium.…”

Section: Resultsmentioning

confidence: 99%

Dissecting the regulation of fructan metabolism in chicory (Cichorium intybus) hairy roots

et al. 2009

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Summary• Fifteen per cent of higher plants accumulate fructans. Plant development, nutritional status and stress exposure all affect fructan metabolism, and while fructan biochemistry is well understood, knowledge of its regulation has remained fragmentary. Here, we have explored chicory (Cichorium intybus) hairy root cultures (HRCs) to study the regulation of fructan metabolism in sink tissues in response to environmental cues.• In standard medium (SM), HRCs did not accumulate inulin. However, upon transfer to high-carbon (C)/low-nitrogen (N) medium, expression of sucrose:sucrose 1-fructosyltransferase (1-SST) and fructan:fructan 1-fructosyltransferase (1-FFT) was strongly induced and inulin accumulated. Upon return to SM, inulin was degraded, together with a coordinate decline of 1-SST and 1-FFT expression. In HRCs, coldinduced expression of fructan 1-exohydrolases (1-FEH I and IIa) was similar to cold induction in taproots, even in the absence of accumulated inulin.• For high-C/low-N induction of 1-SST and 1-FFT, and cold induction of 1-FEH I and IIa, the signaling pathways were addressed. While 1-SST and 1-FFT induction was similarly prevented by inhibitors of Ca 2+ signaling, protein kinases and phosphatases, cold induction of 1-FEH I and IIa revealed distinct signaling pathways.• In summary, this study has established chicory HRCs as a convenient experimental system with which to study the regulation of fructan active enzyme (FAZY) expression in heterotrophic cells.

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Section: Resultsmentioning

confidence: 99%

Dissecting the regulation of fructan metabolism in chicory (Cichorium intybus) hairy roots

et al. 2009

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“…Verification of transformation in hairy-root plants was carried out by using the test for agropine, an amino acid synthesized by TR-DNA genes (De Paolis et al 1985, White et al 1985. Aqueous cellular extracts from the leaves of hairy-root plants were analyzed by high voltage paper electrophoresis, followed by reaction with silver nitrate (Tepfer and Tempe 1981).…”

Section: Methodsmentioning

confidence: 99%

The role of hormones on morphogenesis of thin layer explants from normal and transgenic tobacco plants

Altamura¹,

Capitani²

1992

Physiol Plant

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Altamura, M. M. and Capitani, F. 1992. The role of hormones on morphogenesis of thin layer explaEts from normal and transgenic tobacco plaEts. -Physiol. Plant. 84: 555-560.The organogenic potential of thin layer stem explants of non-reproductive tobacco plants was tested on a hormone-free medium and under various hormonal conditions. A comparison was made between thin layers excised from normal and transgenic plants at the same developmental stage. The transgenic plants were transformed by insertion of TR-and TL-DNA from Agrobacterium rhizogenes 1855 root-inducing plasmid. The aim was to identify hormonal conditions capable of stimulating the expression of the flowering competence present in the differentiated stem tissues at the induced stage before any visible sign of transition to reproductive development. Flower neoformation, observed at the end of the culture period (day 25), occurred on untransformed thin layers only with kinetin treatment. Explants from transgenic plants showed flower bud regeneration on hormone-free medium, indoleacetic acid alone (1 pAf), kinetin alone (1 \M), and most abundantly on indoleacetic acid plus kinetin (1 nM each). No flower formation was observed on indolebutyric add plus kinetin (10 \iM and 0.1 \M, respectively) in both normal and transgenic explants. The latter treatment enhanced rooting instead, above all in the transgenic explants. On hormone-free medium vegetative bud formation was well expressed both by untransformed and transgenic explants, and enhanced by the combined, equimolar concentrations of indoleacetic acid and kinetin. The results show that cytokinin allows flowering in florally determined stem explants from normal plants. In the transgenic explants, the flowering response increases when indoleacetic acid is added to cytokmin, thus suggesting a role for auxin in enhancing the expression of the florally determined state in thin cell layers of non-reproductive plants.

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“…With regard to the latter, the TL-DNA encodes up to 18 potential genes (Slightom et al, 1986;Nemoto et al, 2009), among which only four have an essential role in the formation of hairy roots, i.e. the root locus genes A-D (rolA-rolD) (White et al, 1985;Shen et al, 1988;Nilsson & Olsson, 1997;Nemoto et al, 2009). The single T-DNA of mannopineand cucumopine-type Ri plasmids shows homology to this TL-DNA and also contains the rol locus (Brevet & Temp e, 1988;Chandra, 2012).…”

Section: Infection Processmentioning

confidence: 99%

Rhizogenic agrobacteria in hydroponic crops: epidemics, diagnostics and control

Bosmans

Moerkens

Wittemans³

et al. 2017

Plant Pathology

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Rhizogenic Agrobacterium biovar 1, harbouring an Ri‐plasmid (root‐inducing plasmid), is the causative agent of hairy root disease (HRD) in the hydroponic cultivation of tomato, cucumber and aubergine. The disease is characterized by extensive root proliferation leading to strong vegetative growth and, in severe cases, substantial losses in marketable yield. Agrobacterium biovar 1 is a heterogeneous group of agrobacteria and includes at least 10 genomospecies, among which at least four (G1, G3, G8 and G9) have been associated with HRD in hydroponically grown vegetables. This review has synthesized the current knowledge on rhizogenic Agrobacterium biovar 1, including infection process, current taxonomic status, genetic and phenotypic diversity, detection methods and strategies for disease control. With regard to the latter, symptom reduction and prevention of infection through cultivation methods and chemical disinfection (e.g. by the use of chlorine‐based disinfectants and hydrogen peroxide) are discussed and biocontrol strategies are elaborated on. Recent research has led to the identification of a phylogenetically related clade of Paenibacillus strains that have antagonistic activity against rhizogenic Agrobacterium biovar 1 strains, holding great potential for HRD control. Finally, possible directions for future research are proposed.

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Molecular and genetic analysis of the transferred DNA regions of the root-inducing plasmid of Agrobacterium rhizogenes

Cited by 478 publications

References 44 publications

Dissecting the regulation of fructan metabolism in chicory (Cichorium intybus) hairy roots

Dissecting the regulation of fructan metabolism in chicory (Cichorium intybus) hairy roots

The role of hormones on morphogenesis of thin layer explants from normal and transgenic tobacco plants

Rhizogenic agrobacteria in hydroponic crops: epidemics, diagnostics and control

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