Rhizogenic agrobacteria in hydroponic crops: epidemics, diagnostics and control

Bosmans, Lien; Moerkens, Rob; Wittemans, Lieve; Mot, René De; Rediers, Hans; Lievens, Bart

doi:10.1111/ppa.12687

Cited by 25 publications

(15 citation statements)

References 80 publications

(114 reference statements)

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“…To confirm this hypothesis, further studies with genomic sequencing should be performed. Bacteria belonging to G3, to our knowledge, have not been reported to cause crown gall but have been reported to be the causal agents of crazy root disease (Bosmans et al, 2015(Bosmans et al, , 2017.…”

Section: Resultsmentioning

confidence: 81%

“…This disease infects dicotyledons and was first described as a soilborne disease in apples (Riker et al, 1930) at the beginning of the twentieth century. In recent years, crazy roots have been noted mainly in tomatoes and cucumbers but have also been found in melons and aubergines in the United Kingdom, France (Weller et al, 2000), New Zealand, Greece, Japan (Sawada & Azegami, 2014), Russia (Ignatov et al, 2016) and Belgium (Bosmans et al, 2015(Bosmans et al, , 2017.…”

Section: Introductionmentioning

confidence: 99%

“…Based on reports of crazy roots in different plant species, it is known that the bacteria causing this disease carry root-inducing plasmids (Ri-plasmids). Bacteria that cause this disease and belong to the genus Agrobacterium have been classified mainly as Agrobacterium bv 1 genomospecies: G1, G3, G7, G8, and G9 (Sawada & Azegami, 2014;Bosmans et al, 2015Bosmans et al, , 2017Ignatov et al, 2016). Among other species known to possess causal agents of crazy root disease, Rhizobium rhizogenes (formerly Agrobacterium biovar 2) and Allorhizobium vitis (formerly Agrobacterium vitis or Agrobacterium biovar 3) have also been reported, and both species are also known to be causal agents of crown gall disease (Weller et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

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Identification of the causal agents of crazy root disease on hydroponically cultivated cucumber plants in Poland

Warabieda

Mikiciński

Oleszczak³

et al. 2021

Eur J Plant Pathol

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In April 2019, hydroponically cultivated cucumber plants with characteristic symptoms of crazy root disease were found in two different commercial production cucumber greenhouses in Poland. Due to excessive and inappropriate root growth, this disease led to a reduction in yield and deterioration of the general conditions of infected plants. Bacteria isolated from the roots were subjected to a morphological evaluation, as well as molecular, biochemical and pathogenicity tests. To identify the bacteria causing the disease, Agrobacterium-like colonies were subjected to PCR with primers complementary to the pathogenicity-related genes located on the crazy root-inducing plasmid (Ri-plasmid): the virD2A + virD2E primers complementary to the virD2 gene and the rolBF + rolBR primers complementary to the rolB gene. The pathogenicity of the isolated strains was studied in sunflowers and cucumbers. Twelve strains positive for the Ri plasmid, as determined by PCR, and pathogenic to sunflowers were identified based on sequence analysis of the 16S rRNA and recA genes. One strain was classified as belonging to the genus Pararhizobium, three to Rhizobium, and eight to Agrobacterium biovar 1, with the highest similarity to genomospecies G3. The results of the analyses suggest that these strains may belong to a new, thus far, undescribed species. To confirm this hypothesis, further phylogenetic studies are required.

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Section: Resultsmentioning

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification of the causal agents of crazy root disease on hydroponically cultivated cucumber plants in Poland

Warabieda

Mikiciński

Oleszczak³

et al. 2021

Eur J Plant Pathol

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“…On the other hand, various nonpathogenic Agrobacterium spp. have been found lacking those plasmids [64]. Indeed, numerous Agrobacterium spp.…”

Section: Microbial Variation Of Core Otus As Influenced By Luimentioning

confidence: 99%

Definition of Core Bacterial Taxa in Different Root Compartments of Dactylis glomerata, Grown in Soil under Different Levels of Land Use Intensity

et al. 2020

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Plant-associated bacterial assemblages are critical for plant fitness. Thus, identifying a consistent plant-associated core microbiome is important for predicting community responses to environmental changes. Our target was to identify the core bacterial microbiome of orchard grass Dactylis glomerata L. and to assess the part that is most sensitive to land management. Dactylis glomerata L. samples were collected from grassland sites with contrasting land use intensities but comparable soil properties at three different timepoints. To assess the plant-associated bacterial community structure in the compartments rhizosphere, bulk soil and endosphere, a molecular barcoding approach based on high throughput 16S rRNA amplicon sequencing was used. A distinct composition of plant-associated core bacterial communities independent of land use intensity was identified. Pseudomonas, Rhizobium and Bradyrhizobium were ubiquitously found in the root bacterial core microbiome. In the rhizosphere, the majority of assigned genera were Rhodoplanes, Methylibium, Kaistobacter and Bradyrhizobium. Due to the frequent occurrence of plant-promoting abilities in the genera found in the plant-associated core bacterial communities, our study helps to identify “healthy” plant-associated bacterial core communities. The variable part of the plant-associated microbiome, represented by the fluctuation of taxa at the different sampling timepoints, was increased under low land use intensity. This higher compositional variation in samples from plots with low land use intensity indicates a more selective recruitment of bacteria with traits required at different timepoints of plant development compared to samples from plots with high land use intensity.

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“…Rhizogenic Agrobacterium biovar 1 is the causal agent of hairy root disease (HRD), which affects many hydroponically-grown crops, including tomato, cucumber, eggplant, and bell pepper [1][2][3]. Application of hydrogen peroxide is currently the most used management strategy to reduce HRD.…”

Section: Introductionmentioning

confidence: 99%

Optimizing Biocontrol Activity of Paenibacillus xylanexedens for Management of Hairy Root Disease in Tomato Grown in Hydroponic Greenhouses

Vargas

Bosmans

Kerckhove³

et al. 2021

Agronomy

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Hairy root disease (HRD) caused by rhizogenic Agrobacterium biovar 1 strains affect tomato, cucumber, eggplant, and bell pepper grown in hydroponic greenhouses and can cause considerable yield losses worldwide. Recently, Paenibacillus xylanexedens strains (ST15.15/027 and AD117) with antagonistic activity against rhizogenic agrobacteria were identified. In this study, we present results of greenhouse trials of two consecutive growing seasons (2019 and 2020) to examine the potential of these two biocontrol organisms (BCOs) under practical conditions. BCO-treatment at a 107 colony forming units (CFU)/mL density resulted in a considerable reduction of the HRD infestation rate, confirming the biocontrol potential of the two P. xylanexedens strains. Results revealed that a single BCO strain (ST15.15/027) performed equally well as the mixed inoculum of both strains. The same level of biocontrol activity was even achieved when the BCO inoculum density was reduced to 105 CFU/mL. qPCR analysis further showed that Paenibacillus was still present in rockwool substrate near the end of both trials, indicating that they persist well in a rockwool environment and that application at the start of the trial is sufficient to protect tomato plants until the end of the trial. Altogether, these results are highly valuable for further optimization and exploitation of P. xylanexedens as a biocontrol product for the control of HRD in hydroponic greenhouses.

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Rhizogenic agrobacteria in hydroponic crops: epidemics, diagnostics and control

Cited by 25 publications

References 80 publications

Identification of the causal agents of crazy root disease on hydroponically cultivated cucumber plants in Poland

Identification of the causal agents of crazy root disease on hydroponically cultivated cucumber plants in Poland

Definition of Core Bacterial Taxa in Different Root Compartments of Dactylis glomerata, Grown in Soil under Different Levels of Land Use Intensity

Optimizing Biocontrol Activity of Paenibacillus xylanexedens for Management of Hairy Root Disease in Tomato Grown in Hydroponic Greenhouses

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