Molecular and histological changes following central retinal artery occlusion in a mouse model

Goldenberg-Cohen, Nitza; Dadon, S.; Avraham, B.R.; Kramer, Michal; Hasanreisoğlu, Murat; Eldar, Ido; Weinberger, Dov; Bahar, Irit

doi:10.1016/j.exer.2008.06.014

Cited by 47 publications

(36 citation statements)

References 43 publications

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“…Many ocular diseases are associated with retinal ischemia such as ischemic optic neuropathies, diabetic retinopathy, glaucoma, and ocular ischemic syndrome. Furthermore, these ocular diseases caused neuronal cell death in the inner retina, which has been observed in the GCL, INL and outer nuclear layer of the retina [1,2,25,26]. In the present study, the number of cells in the RGC and the thickness of the IRL were decreased after IR injury.…”

Section: Discussionsupporting

confidence: 55%

Neuroprotective Effects of Crocin against Oxidative Stress Induced by Ischemia/Reperfusion Injury in Rat Retina

Chen

Yang

2015

Ophthalmic Res

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Aims: Crocin, a pharmacologically active component of Crocus sativus L. (saffron), has been reported to be useful in the treatment of oxidative stress injury. In the present study, we investigated the antioxidative effect of crocin and the change of the ERK signaling pathway on rat retina induced by ischemia/reperfusion (IR) injury. Methods: Crocin was pretreated 30 min before and once daily after retinal IR injury by intraperitoneal injection. The retinal morphological damage was observed by hematoxylin and eosin (HE) staining. The number of retinal ganglion cells (RGCs) was counted by Brn-3a immunofluorescence staining. The antiapoptotic effect of crocin was determined by detecting cleaved caspase-3 protein levels by means of Western blot and immunohistochemical analysis. Furthermore, retinas were also used for the determination of malondialdehyde (MDA) levels, glutathione (GSH) levels, total superoxide dismutase (T-SOD), and reactive oxygen species (ROS). The phosphorylated ERK (p-ERK) protein level was determined by Western blot and immunohistochemical analysis. Results: Our results showed that crocin treatment (50 mg/kg) significantly inhibited the decrease of retinal thickness through HE staining and protected RGCs from decreasing. Compared with the IR + vehicle group, crocin treatment significantly decreased cleaved caspase-3 and p-ERK protein expression by Western blot analysis and immunohistochemistry. Immunohistochemical staining for cleaved caspase-3 and p-ERK in the retinal sections showed positive cells were present in the RGC layer and inner nuclear layer after IR injury. Similarly, crocin (50 mg/kg) treatment also significantly increased the level of activity of GSH, enhanced the activity of T-SOD, and decreased the activity level of ROS and MDA after IR injury. Conclusions: These findings demonstrated that crocin treatment could notably protect the retina from damage induced by IR. It might be related to crocin antioxidant and antiapoptotic properties in the retina.

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Section: Discussionsupporting

confidence: 55%

Neuroprotective Effects of Crocin against Oxidative Stress Induced by Ischemia/Reperfusion Injury in Rat Retina

Chen

Yang

2015

Ophthalmic Res

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“…However, no loss of cells within the ischemic retina was observed 1 d after reperfusion in adult SV129EV mice (15). Central retinal artery occlusion significantly reduced GCL cell counts and retinal thickness at 21 d after ischemic injury in adult mice (11). In our study, there were many pyknotic cells …”

Section: Discussionmentioning

confidence: 61%

“…Several retinal ischemia models, including high intraocular pressure, vascular occlusion, and intravitreal injection of NMDA (N-methyl-D-aspartate), have been well established in adult rodents. A common hallmark for these conditions is the progressive degeneration and the final neuronal loss in the ganglion cell layer (GCL) (8)(9)(10)(11)(12)(13). However, it remains to be determined whether the developing eyes are vulnerable to HI damage at both pathological and functional levels.…”

mentioning

confidence: 99%

Hypoxic–ischemic retinal injury in rat pups

Huang

Hung

et al. 2012

Pediatr Res

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Background: Visual loss associated with brain damage, especially hypoxic-ischemic (hI) encephalopathy, is the most common cause of visual impairment in children in developed countries. We hypothesized that hI insults can cause long-term damage in immature eyes. Methods: In postnatal day 7 rat pups, hI was induced by unilateral common carotid artery ligation followed by hypoxia. Retina damage was assessed by electroretinography (eRG) and cell counting. Neuronal injury and astrogliosis were evaluated by terminal deoxynucleotidyl transferase nick-end labeling, cleaved caspase 3, eD1, and glial fibrillary acidic protein immunostaining. results: We observed rapid and persistently extensive injuries in the ganglia cell layer (GcL), inner plexiform layer, and inner nuclear layer (INL) in ipsilateral retinas after hI injury, corresponding to the marked alteration in eRG. hI insult caused prominent microglial and Műller cell activation in ipsilateral inner retinas. Neuronal death in the GcL and INL after hI injury was mainly apoptotic, involving caspase-dependent pathways. conclusion: Our study demonstrated the first evidence of hI retinal damage at both the pathological and functional level using the Vannucci model in neonatal rats. Because retinal damage is often associated with hI injury, it is important to demonstrate that a particular neuroprotective strategy effectively preserves the retina in addition to the brain.

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“…8,9 His to pat ho logic chan ges are li mi ted to the ner ve fi ber and gang li on cell la yer. 10 The re is pro fo und loss of gang li on cells and dif fu se ede ma in si de the in ner re tinal la yers while litt le chan ge is se en in the de e per re ti nal la yers supp li ed by the cho ro i dal ves sels. Sup por tingly, OCT de mons tra tes in cre a sed ref lecti vity in the in ner re ti na with dec re a sed ref lec tivity of the ou ter re ti nal la yers.…”

Section: Discussionmentioning

confidence: 99%

Autofluorescence Imaging in Eyes with Various Types of Retinal Artery Occlusion: Case Report

Kaya¹,

Yaman²,

Öner³

et al. 2011

Turkiye Klinikleri J Med Sci

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Molecular and histological changes following central retinal artery occlusion in a mouse model

Cited by 47 publications

References 43 publications

Neuroprotective Effects of Crocin against Oxidative Stress Induced by Ischemia/Reperfusion Injury in Rat Retina

Neuroprotective Effects of Crocin against Oxidative Stress Induced by Ischemia/Reperfusion Injury in Rat Retina

Hypoxic–ischemic retinal injury in rat pups

Autofluorescence Imaging in Eyes with Various Types of Retinal Artery Occlusion: Case Report

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