Molecular and immunochemical phylogeny of Verticillium species

Pantou, Malena P.; Струнникова, О. К.; Shakhnazarova, V. Yu.; Vishnevskaya, N. A.; Papalouka, Vasiliki; Typas, Milton A.

doi:10.1017/s0953756205003345

Cited by 34 publications

(31 citation statements)

References 56 publications

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“…Several studies using RAPD or AFLP markers, as well as ribosomal DNA sequences contend that because the long-spored crucifer strains cluster separately from V. dahliae, the crucifer strains ought to be raised to the level of a distinct species (49,76,107,172). The decision on where to split well-delineated groups into distinct species is subjective (154).…”

Section: Contemporary Taxonomic Controversymentioning

confidence: 99%

“…We note that by clustering with the established species the long-spored strains would then, at least by looking at this one locus, not be considered a distinct species, as is clear from Figure 1. Because divergence at one locus is only representative of the evolutionary history of that locus, not the species as a whole, Fahleson et al (49) and Pantou et al (107) used multi-gene sequences from nuclear and mitochondrial regions to estimate the phylogenetic relatedness of various species of Verticillium. We compared the aligned sequences generated by the latter two studies and found no more than three single nucleotide polymorphisms (SNPs) separating long-spored crucifer strains of V. dahliae or V. albo-atrum.…”

Section: Contemporary Taxonomic Controversymentioning

confidence: 99%

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Diversity, Pathogenicity, and Management of Verticillium Species

Klosterman

Atallah

Vallad

et al. 2009

Annu. Rev. Phytopathol.

649

564

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The genus Verticillium encompasses phytopathogenic species that cause vascular wilts of plants. In this review, we focus on Verticillium dahliae, placing emphasis on the controversy surrounding the elevation of a long-spored variant as a new species, recent advances in the analysis of compatible and incompatible interactions, highlighted by the use of strains expressing fluorescent proteins, and the genetic diversity among Verticillium spp. A synthesis of the approaches to explore genetic diversity, gene flow, and the potential for cryptic recombination is provided. Control of Verticillium wilt has relied on a panoply of chemical and nonchemical strategies, but is beset with environmental or site-specific efficacy problems. Host resistance remains the most logical choice, but is unavailable in most crops. The genetic basis of resistance to Verticillium wilt is unknown in most crops, as are the subcellular signaling mechanisms associated with Ve-mediated, race-specific resistance. Increased understanding in each of these areas promises to facilitate management of Verticillium wilts across a broad range of crops.

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Section: Contemporary Taxonomic Controversymentioning

confidence: 99%

Section: Contemporary Taxonomic Controversymentioning

confidence: 99%

Diversity, Pathogenicity, and Management of Verticillium Species

Klosterman

Atallah

Vallad

et al. 2009

Annu. Rev. Phytopathol.

649

564

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“…A part of the translation elongation factor 1-alpha gene (EF1a) was amplified using primers EF1-983F and EF1-1567R (Rehner and Buckley 2005). The DNA-dependent RNA polymerase II largest subunit gene (rpb1) was partially amplified using PCR primers Vrpb1F and Vrpb1R, which we designed from the sequence of V. tricorpus (AY555904) reported by Pantou et al (2005). Nucleotide sequences of primers are presented in Table S1 in Electronic Supplementary Material.…”

Section: Pathogenicity Testmentioning

confidence: 99%

Verticillium tricorpus causing lettuce wilt in Japan differs genetically from California lettuce isolates

et al. 2010

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In January 2002, Verticillium wilt of lettuce (Lactuca sativa L.) caused by Verticillium tricorpus occurred in upland paddy fields in Hyogo Prefecture for the first time in Japan. This fungal species was first isolated from lettuce in California, USA. In the present study, the genetic relationships between the American and Japanese isolates of V. tricorpus from lettuce were analyzed to determine whether the pathogen could have migrated to Japan from the USA, the major lettuce-seed supplier for Japan. Nucleotide sequences of the rDNA internal transcribed spacer regions, as well as the genes coding for translation elongation factor 1-alpha and RNA polymerase II were compared among American and Japanese V. tricorpus isolates from lettuce. The Japanese isolates of V. tricorpus were distinct from the American. Random amplified polymorphic DNA analyses also supported this conclusion. These results demonstrated that Verticillium wilt on lettuce caused by V. tricorpus in Japan was not related to the isolates causing the disease in California.

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“…There are no known publications in the available literature that deal with a comparable detection of fungal pathogens using ELISA and PCR. In oilseed rape, these methods are mostly used separately to detect viruses (Breitenmoser et al, 2011;Yu et al, 2015), evaluate seed quality (Wagner et al, 2012), assess phylogenetic relationships within Verticillium species (Pantou et al 2005), and determine fungicide residues in oilseed rape leaves as part of monitoring fungicide activity and current concentrations in relation to the timing and effectiveness of treatments against Leptosphaeria maculans and Pyrenopeziza brassicae (Coules and Rossall, 2003). The match rate of 60 % between the methods in analysing V. longisporum is not poor, but for practical use a higher match rate would be necessary in cases where samples were to be analysed in various laboratories using only one of these techniques.…”

Section: Discussionmentioning

confidence: 99%

Detecting Pathogens of Verticillium Wilt in Winter Oilseed Rape Using ELISA and PCR - Comparison of the Two Methods and With Visual Stand Evaluation

Spitzer

Matušinsky

2017

Acta Univ. Agric. Silvic. Mendelianae Brun.

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During 2013-2015, oilseed rape samples from various locations in the Czech Republic were analysed for the presence of Verticillium wilt. Samples were evaluated in the lab using ELISA and PCR as well as by visual evaluation during the samples collection. A comparison of detection match in individual methods also was made. ELISA and PCR matched in detecting Verticillium wilt in 60 % of cases. For practical use, a higher match rate would be necessary in cases where samples were to be analysed in various laboratories using only one of these techniques. The possibility was demonstrated to use kits as well as primers not targeted specifically to just Verticillium longisporum but recording both main species on oilseed rape (V. longisporum and V. dahliae). The match rates of lab analyses with visual evaluation of stand infection were surprisingly high at 62 % for ELISA and 77 % for PCR. All three stand evaluation methods matched in 56 % of cases.

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Molecular and immunochemical phylogeny of Verticillium species

Cited by 34 publications

References 56 publications

Diversity, Pathogenicity, and Management of Verticillium Species

Diversity, Pathogenicity, and Management of Verticillium Species

Verticillium tricorpus causing lettuce wilt in Japan differs genetically from California lettuce isolates

Detecting Pathogens of Verticillium Wilt in Winter Oilseed Rape Using ELISA and PCR - Comparison of the Two Methods and With Visual Stand Evaluation

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