Molecular and Kinetic Characterization of MOX-9, a Plasmid-Mediated Enzyme Representative of a Novel Sublineage of MOX-Type Class C β-Lactamases

Piccirilli, Alessandra; Antonelli, Alberto; D’Andrea, Marco Maria; Cherubini, Sabrina; Perilli, Mariagrazia; Rossolini, Gian María

doi:10.1128/aac.00595-22

Cited by 3 publications

(5 citation statements)

References 24 publications

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“…(Ye et al, 2010;Piotrowska et al, 2017). In addition, bla MOX-9 was previously found in the transposon of A. media species and considered as a mobile antibiotic resistance gene (Ebmeyer et al, 2019;Piccirilli et al, 2022), whereas this gene detected in our study was encoded in the chromosome of the A. media strain. Moreover, the presence of bla AQU was observed in clinical isolates of A. dhakensis showing cefotaxime resistance (Wu et al, 2013), and cepS together with bla OXA-12 and cphA7 genes were detected in clinical strains of A. hydrophila strains showing carbapenem resistance (Hilt et al, 2020).…”

Section: Discussionmentioning

confidence: 47%

“…Class C β-lactamases have been identified in both chromosomes and plasmids of Aeromonas spp. originating from various sources (Piotrowska et al, 2017;Ebmeyer et al, 2019;Piccirilli et al, 2022). The class C β-lactamases genes detected in our study were bla MOX-9 (in A. media), bla MOX-15 (in A. caviae), bla AQU (in A. dhakensis), and cepS (in A. hydrophila).…”

Section: Discussionmentioning

confidence: 65%

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Whole genome sequence analysis of Aeromonas spp. isolated from ready-to-eat seafood: antimicrobial resistance and virulence factors

et al. 2023

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Aeromonas are widespread in aquatic environments and are considered emerging pathogens in humans and animals. Multidrug resistant (MDR) Aeromonas circulating in the aquatic environment and food production chain can potentially disseminate antimicrobial resistance (AMR) to humans via the foodborne route. In this study, we aimed to investigate AMR and virulence factors of 22 Aeromonas strains isolated from ready-to-eat (RTE) seafood. A multilocus phylogenetic analysis (MLPA) using the concatenated sequences of six housekeeping genes (gyrB, rpoD, gyrA, recA, dnaJ, and dnaX) in the 22 Aeromonas genomes and average nucleotide identity (ANI) analysis revealed eight different species; A. caviae, A. dhakensis, A. hydrophila, A. media, A. rivipollensis, A. salmonicida, A. bestiarum, and A. piscicola. The presence of virulence genes, AMR genes and mobile genetic elements (MGEs) in the Aeromonas genomes was predicted using different databases. Our data showed that the genes responsible for adherence and motility (Msh type IV pili, tap type IV pili, polar flagella), type II secretion system (T2SS) and hemolysins were present in all strains, while the genes encoding enterotoxins and type VI secretion system (T6SS) including major effectors were highly prevalent. Multiple AMR genes encoding β-lactamases such as cphA and blaOXA were detected, and the distribution of those genes was species-specific. In addition, the quinolone resistance gene, qnrS2 was found in a IncQ type plasmid of the A. rivopollensis strain A539. Furthermore, we observed the co-localization of a class I integron (intl1) with two AMR genes (sul1 and aadA1), and a Tn521 transposon carrying a mercury operon in A. caviae strain SU4-2. Various MGEs including other transposons and insertion sequence (IS) elements were identified without strongly associating with detected AMR genes or virulence genes. In conclusion, Aeromonas strains in RTE seafood were potentially pathogenic, carrying several virulence-related genes. Aeromonas carrying multiple AMR genes and MGEs could potentially be involved in the dissemination and spread of AMR genes to other bacterial species residing in the same environment and possibly to humans. Considering a One-Health approach, we highlight the significance of monitoring AMR caused by Aeromonas circulating in the food chain.

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Section: Discussionmentioning

confidence: 47%

Section: Discussionmentioning

confidence: 65%

Whole genome sequence analysis of Aeromonas spp. isolated from ready-to-eat seafood: antimicrobial resistance and virulence factors

et al. 2023

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“…Biochemical characterization of this MOX-9 gene revealed a strong binding preference for cephalosporins and cephamycins. By comparing MOX-9 binding affinity and its hydrolysis activity to other more common MOX-type enzymes, this study not only demonstrated the variations that exist within this family of resistance genes but also provided a genetic context by which resistance genes can be easily mobilized onto transmissible plasmids and horizontally shared among other organisms (Piccirilli et al 2022 ).…”

Section: Mechanisms Of Aeromonas -Associated Drug ...mentioning

confidence: 85%

“…When facing antimicrobial challenges, Aeromonas spp. employ numerous AMR strategies of evasion including mutations of the drug targets themselves and, in some cases, expression of specific antibiotic degrading enzymes (if their encoding genes are present) (Piccirilli et al 2022 ; Rasmussen and Bush 1997 ; Sinha et al 2004 ). Aeromonas spp.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance in aeromonads and new therapies targeting quorum sensing

Neil,

Cheney,

Rosenzweig

et al. 2024

Appl Microbiol Biotechnol

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Aeromonas species (spp.) are well-known fish pathogens, several of which have been recognized as emerging human pathogens. The organism is capable of causing a wide spectrum of diseases in humans, ranging from gastroenteritis, wound infections, and septicemia to devastating necrotizing fasciitis. The systemic form of infection is often fatal, particularly in patients with underlying chronic diseases. Indeed, recent trends demonstrate rising numbers of hospital-acquired Aeromonas infections, especially in immuno-compromised individuals. Additionally, Aeromonas-associated antibiotic resistance is an increasing challenge in combating both fish and human infections. The acquisition of antibiotic resistance is related to Aeromonas’ innate transformative properties including its ability to share plasmids and integron-related gene cassettes between species and with the environment. As a result, alternatives to antibiotic treatments are desperately needed. In that vein, many treatments have been proposed and studied extensively in the fish-farming industry, including treatments that target Aeromonas quorum sensing. In this review, we discuss current strategies targeting quorum sensing inhibition and propose that such studies empower the development of novel chemotherapeutic approaches to combat drug-resistant Aeromonas spp. infections in humans. Key points • Aeromonas notoriously acquires and maintains antimicrobial resistance, making treatment options limited. • Quorum sensing is an essential virulence mechanism in Aeromonas infections. • Inhibiting quorum sensing can be an effective strategy in combating Aeromonas infections in animals and humans.

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“…Cephalosporinases originated from environmental strains, like bla CTX-M from environmental Kluyvera ( 9 ) and bla CMY/MOX from Aeromonas , have spread to multiple human pathogens ( 10 ). From a “One Health” perspective, it is important to highlight the role of the aquatic environment as a hidden reservoir of ARGs.…”

Section: Lettermentioning

confidence: 99%

First report of environmental bla _PAC-1 -carrying Aeromonas enteropelogenes

Zhong,

Guo,

Thong

et al. 2023

Microbiol Spectr

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The bla PAC-1 has been reported in Central Asia and Europe countries like Afghanistan and France in Aeromonas caviae and Pseudomonas aeruginosa strains from animals and patients, respectively. However, there is no record of bla PAC-1 -carrying strain from the natural environment, and bla PAC-1 -carrying Aeromonas has not been reported in the Asia Pacific. Here, we report the first known enviromental bla PAC-1 -carrying Aeromonas enteropelogenes in the world from reservoir water in Singapore. We have performed a comprehensive genetic environment alignment and comparison of bla PAC-1 between our strain and other strains from different countries and sources and found the bla PAC-1 located on a highly conserved gene cluster. We suggest that environmental Aeromonas strains may act as a hidden reservoir involved in the circulating of bla PAC-1 . The finding of conserved bla PAC-1 cluster also suggested the existence of multiple transmission pathways of bla PAC-1 in the Asia-Pacific region, involving multiple sources and different species.

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Molecular and Kinetic Characterization of MOX-9, a Plasmid-Mediated Enzyme Representative of a Novel Sublineage of MOX-Type Class C β-Lactamases

Cited by 3 publications

References 24 publications

Whole genome sequence analysis of Aeromonas spp. isolated from ready-to-eat seafood: antimicrobial resistance and virulence factors

Whole genome sequence analysis of Aeromonas spp. isolated from ready-to-eat seafood: antimicrobial resistance and virulence factors

Antimicrobial resistance in aeromonads and new therapies targeting quorum sensing

First report of environmental bla _PAC-1 -carrying Aeromonas enteropelogenes

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Molecular and Kinetic Characterization of MOX-9, a Plasmid-Mediated Enzyme Representative of a Novel Sublineage of MOX-Type Class C β-Lactamases

Cited by 3 publications

References 24 publications

Whole genome sequence analysis of Aeromonas spp. isolated from ready-to-eat seafood: antimicrobial resistance and virulence factors

Whole genome sequence analysis of Aeromonas spp. isolated from ready-to-eat seafood: antimicrobial resistance and virulence factors

Antimicrobial resistance in aeromonads and new therapies targeting quorum sensing

First report of environmental bla PAC-1 -carrying Aeromonas enteropelogenes

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Product

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First report of environmental bla _PAC-1 -carrying Aeromonas enteropelogenes