Molecular and morphological characterisation of Aphelenchoides paraxui n. sp. (Nematoda: Aphelenchoididae) isolated from Quercus brantii in western Iran

Esmaeili, Mehrab; Heydari, Ramin; Fang, Yiwu; Li, Hongmei

doi:10.1007/s10658-017-1212-y

Cited by 8 publications

(4 citation statements)

References 37 publications

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“…The species A. paraxui is illustrated by centrally located valve at median bulb and a conus equal to shaft in line drawings (Esmaeili et al, 2017a), while light microphotograps show posteriorly located well-developed valve in metacorpus, and a conus, shorter than the shaft.…”

Section: Remarksmentioning

confidence: 99%

Description of Aphelenchoides giblindavisi n. sp. (Nematoda: Aphelenchoididae), and Proposal for a New Combination

Aliramaji

Pourjam

Álvarez-Ortega

et al. 2018

Journal of Nematology

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One new and one known species of the genus Aphelenchoides from Iran are studied. Aphelenchoides giblindavisi n. sp. is mainly characterized by having five lines in the lateral fields at mid-body, and a single mucro with several tiny nodular protuberances, giving a warty appearance to it, as revealed by detailed scanning electron microscopic (SEM) studies. The new species is further characterized by having a body length of 546 to 795 μ m in females and 523 to 679 μ m in males, rounded lip region separated from the rest body by a shallow depression, 10 to 11 μm long stylet with small basal swellings, its conus shorter than the shaft (m = 36-43), 52 to 69 µm long postvulval uterine sac (PUS), males with 16 to 18 μ m long arcuate spicules, and three pairs of caudal papillae. The new species was morphologically compared with two species of the genus having five lines in the lateral fields namely A. paramonovi and A. shamimi and species having a warty-surfaced mucro at tail end and similar morphometric data ranges. The morphological features and morphometrics of the second studied species, A. helicus, agreed well with the data given for the type population. However, detailed study of fresh females revealed it has three drop-shaped stylet knobs and long PUS, making it typologically similar to the genus Robustodorus, meriting its taxonomic revision, i.e., transferring to it. In molecular phylogenetic analyses using partial small and large subunit ribosomal RNA gene (SSU and LSU rDNA) sequences, the new species formed a clade with A. heidelbergi in both SSU and LSU D2-D3 trees. The species A. helicus, however, clustered inside a well-supported clade of the genus Robustodorus in both trees, corroborating its newly proposed taxonomic placement as Robustodorus helicus n. comb.

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Section: Remarksmentioning

confidence: 99%

Description of Aphelenchoides giblindavisi n. sp. (Nematoda: Aphelenchoididae), and Proposal for a New Combination

Aliramaji

Pourjam

Álvarez-Ortega

et al. 2018

Journal of Nematology

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“…Compared with A. xui Wang, Wang, Gu, Wang, and Li, 2013, it differs in number of lateral field incisures (6 vs. 5) and spicule (rostrum conical vs. rostrum rounded). Compared with A. paraxui Esmaeili, Heydari, Fang, and Li, 2017, it differs in the longer female body (699–1068 vs. 500–660 μm), longer stylet (12.0–13.5 vs. 8–9 μm), and number of lateral field incisures (6 vs. 4). Compared with A. smolae Wang, Wang, Gu, Wang, and Li, 2013, it differs in shorter stylet (12.0–13.5 vs. 13.0–14.9 μm) and number of lateral field incisures (6 vs. 4).…”

Section: Resultsmentioning

confidence: 89%

“…Besides , A. persicus sp. n. along with A. varicaudatus Ibrahim and Hooper, 1994; A. xui , A. fragariae (Ritzema Bos, 1890) Christie, 1932; A. macrospica Golhasan, Heydari, Esmaeili, and Miraeiz, 2017; A. eldaricus Esmaeili, Heydari, Golhasan, and Kanzaki 2017; A. paraxui, and A. iranicus Golhasan, Heydari, Álvarez-Ortega, Esmaeili, Castillo, and Palomares-Rius, 2016; and several unidentified Aphelenchoides formed a clade with a 0.79 posterior probability support values.…”

Section: Resultsmentioning

confidence: 99%

Description of Aphelenchoides persicus sp. n. (Nematoda: Aphelenchoididae) from Iran

Aliramaji,

Taheri,

Shokoohi

2023

J. Helminthol.

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During a survey on the soil nematodes, a population of the genus Aphelenchoides was collected around the rhizosphere of persimmon in Guilan Province, Iran. The morphological and molecular characters confirmed the new species, namely A. persicus sp. n. The new species is characterized by a female body length (699–1068 μm), lip region offset from the rest of the body by a slight constriction, lateral fields with six incisures, stylet 12–13.5 μm long, with a clear basal swelling, excretory pore ca 1.5 metacorpal length posterior to base of the metacorpus, post uterine sac elongate, about 4–7 times than the vulval body diameter; conical female tail with a single centrally located mucron with tiny projection close to the tail tip, male body length (663–908 μm), and spicule well developed with rounded condylus, blunt conical rostrum, and a hook-like tip of dorsal limb. The new species belongs to the Group 2 category of Aphelenchoides species and was similar to seven known species with six lateral field incisures, including A. allius, A. chinensis, A. meghalayensis, A. nechaleos, A. paranechaleos, A. parasexlineatus, and A. sexlineatus. The molecular phylogeny based on 28S rDNA revealed that the new species stands close to A. hamospiculatus (MN931591; MN931592) and two unidentified Aphelenchoides (KY769057; LC583315). The measurements, line illustrations, LM photographs, and phylogenetic analysis are given for the new species.

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“…The sequence identity between A. prairiensis n. sp. and the clustered species was 97% (17–21 bp difference), while A. limberi formed a subclade with Aprutides guidetti Scognamiglio [ 54 ], an A. limberi population sequence from Iran, A. centralis Thorne and Malek [ 56 ], A. unisexus Jain and Singh [ 57 ], and A. paraxui Esmaeili, Heydari, Fang, and Li [ 58 ]. The sequence identity between A. limberi and the clustered species was 97% (15–17 bp difference).…”

Section: Resultsmentioning

confidence: 99%

Discovery and Characterization of Four Aphelenchid Species from Cultivated Regions of Southern Alberta, Canada

Munawar,

Castillo,

Yevtushenko

2024

Microorganisms

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The nematode family Aphelenchoididiae is considered fungal-feeding, predatory, or root hair feeders. Some members of this family are universally present in agricultural landscapes and are an integral part of soil health and conservation studies. In the present soil nematode biodiversity survey, we detected four species of the genera Aphelenchus, Aphelenchoides, and Robustodorus. Because fungal-feeding nematodes from southern Alberta have not previously been reported, we conducted a detailed morphological and molecular investigation, identifying these species as Aphelenchus avenae, Aphelenchoides limberi, Aphelenchoides prairiensis n. sp. and Robustodorus paramegadorus n. sp. The first two species we document as new records from southern Alberta, whereas A. prairiensis n. sp. and R. paramegadorus n. sp. we describe in detail as new taxa. Briefly, A. prairiensis n. sp. is an amphimictic species having 4 lateral lines; hemispherical anteriorly flattened lip region; delicate stylet and swelling-like stylet knobs; excretory pore at the posterior edge of nerve ring. Female tail conical, gradually tapering towards a truncated end with single mucro. Spicule 23.0 (20.0–25.0) µm long having elongated rounded condylus, small, blunt conical rostrum, and lamina that gradually tapers towards the rounded distal end; three pairs of caudal papillae were present on the male tail. Robustodorus paramegadorus n. sp., is a parthenogenetic species with 3 lines in the lateral fields; lip region rounded, anteriorly flattened; stylet robust, with knobs rounded to bean-shaped; excretory pore located posterior to nerve ring; reproductive components were quite indiscernible with a short 24.0 (18.0–27.0) µm post-vulval uterine sac; tail conical, ending with pointed to wedge-shaped tip. We performed molecular characterizations for each species and constructed phylogenetic trees to study the phylogenetic relationship of these aphelenchid species. The discovery of A. prairiensis n. sp. and R. paramegadorus n. sp. indicates that soil nematode diversity is relatively unexplored in southern Alberta. The findings of this study will significantly enhance the identification processes and may contribute towards future soil health and biodiversity efforts.

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Molecular and morphological characterisation of Aphelenchoides paraxui n. sp. (Nematoda: Aphelenchoididae) isolated from Quercus brantii in western Iran

Cited by 8 publications

References 37 publications

Description of Aphelenchoides giblindavisi n. sp. (Nematoda: Aphelenchoididae), and Proposal for a New Combination

Description of Aphelenchoides giblindavisi n. sp. (Nematoda: Aphelenchoididae), and Proposal for a New Combination

Description of Aphelenchoides persicus sp. n. (Nematoda: Aphelenchoididae) from Iran

Discovery and Characterization of Four Aphelenchid Species from Cultivated Regions of Southern Alberta, Canada

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