Molecular and pathological investigations of EHV-1 and EHV-4 infections in horses in Turkey

Turan, Nuri; Yıldırım, Funda; Altan, Eda; Şennazlı, Gülbin; Gürel, Aydın; Diallo, Ibrahim S.; Yılmaz, Hüseyin

doi:10.1016/j.rvsc.2012.01.019

Cited by 15 publications

(25 citation statements)

References 28 publications

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“…The results of the phylogenetic analysis of partial gB gene sequences of two Serbian EHV-1 isolates showed that they were closely related to EHV-1 strains from Turkey, the United Kingdom, the United States, and Japan. The results of Turan et al (2012) indicated that most of the Turkish EHV-1 strains clustered together, whilst one strain was slightly removed from the group clustering with European EHV-1 strains. Ataseven et al (2016) determined that EHV-1 isolates in Turkey associated with abortion were closely related to the European EHV-1 strains.…”

Section: Discussionmentioning

confidence: 97%

“…Equine herpesvirus 1 may cause latent infection in lymphoid tissue and the sensory and trigeminal ganglia, whilst stress, transport and intercurrent disease lead to virus reactivation in asymptomatic carrier horses with the spread of the disease in the equine population as a consequence (Edington et al 1994;Pusterla et al 2010;Negussie et al 2017). Equine herpesvirus 1 causes respiratory disease in young animals, abortion in pregnant mares and neurological disease (Wang et al 2007;Pusterla et al 2010;Turan et al 2012). The presence of EHV-1 in Serbia is known, however, there have been no recent reports of its potential connection to outbreaks of neurological disease or abortion in horses.…”

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confidence: 99%

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Detection and molecular characterization of equine herpesviruses 1, 2, and 5 in horses in the Republic of Serbia

et al. 2018

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The presence of equine herpesviruses 1, 2 and 5 (EHV-1, EHV-2 and EHV-5) was examined in 66 samples of spinal cord, submandibular lymph nodes and spleen of healthy, non-vaccinated abattoir horses from different locations in the Republic of Serbia. Virus isolation was conducted on RK-13 cell line with the confirmation of isolated viral strains by multiplex nested polymerase chain reaction. The cytopathic effect was observed 48–72 h after the first inoculation in 28 (42.4%) organ samples, and after 5 days in 11 other samples (16.7%) that were all confirmed as EHV-1. Four other samples (6.1%) that showed cytopathic effects on day 5 of the third passage were all positive for EHV-5. Additionally, EHV-1, EHV-2, and EHV-5 were directly detected in all organs by multiplex nested PCR in 46 (69.7%), 3 (4.5%), and 7 (10.6%) samples, respectively. The molecular characterization based on nucleotide sequencing of the part of the gB gene showed that Serbian EHV-1 isolates were 100% homogenous and clustered with EHV-1 strains from Turkey, the United Kingdom, the United States, and Japan. The EHV-2 strain from Serbia branched together with Turkish EHV-2 isolates with homogeneity from 96% to 98%. Serbian EHV-5 strains can be separated in one distinct cluster with isolates from Turkey and the United States with homogeneity from 98 to 99%. These data represent the first report of the molecular characterization of EHV-1, EHV-2, and EHV-5 in the horse population of the Republic of Serbia and document the first successful isolation of Serbian EHV-5 strains.

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Section: Discussionmentioning

confidence: 97%

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confidence: 99%

Detection and molecular characterization of equine herpesviruses 1, 2, and 5 in horses in the Republic of Serbia

et al. 2018

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“…These oligonucleotides were diluted 1 in 100,000 before use. Positive controls for EHV-1 detection included previously detected positive samples [17]. Negative controls for TaqMan probe assay included DNA extracted from repeatedly negative samples for EHV-1 and nuclease-free water in place of template.…”

Section: Taqman Probe Assay For Strain Differentiationmentioning

confidence: 99%

“…This has increased in recent years and continues to be a problem for the Turkish horse industry [15][16][17]. However, in Turkey, the involvement of neuropathogenic strains among EHV-1 strains is not known at present, although the importance has been emphasized in many countries [4,8,[18][19][20][21].…”

Section: Introductionmentioning

confidence: 99%

First Report on the Frequency and Molecular Detection of Neuropathogenic EHV-1 in Turkey

Yılmaz

Altan

Turan

et al. 2012

Journal of Equine Veterinary Science

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“…EHV-1 was first isolated during an outbreak of abortion in 1968 in Turkey (6). Subsequently, there have been reports on the epidemiology of EHV-1 based on an outbreak of abortion and respiratory disease (3,4,17,34,35,36). In this report, a genetic analysis of EHV-1 field strains isolated from an abortion outbreak during the 2011 foaling season in vaccinated thoroughbred mares in Turkey was conducted using a novel primer set based on EHV-1gB gene…”

Section: Introductionmentioning

confidence: 99%

Partial sequence of the gB gene of equid herpesvirus type 1 isolates associated with abortion in Turkey

Ataseven¹,

Oğuzoğlu²,

Dinçer³

et al. 2016

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Equid herpesvirus 1 (EHV-1) is a major agent of large-scale outbreaks of abortion, and these abortions have been described as sporadic or epidemic cases in mares, generally during the last trimester of pregnancy. In this study, the partial characterization based on the glycoprotein B (gB) gene of Turkish (TR) EHV-1 field strains isolated from an abortion outbreak during the 2011 foaling season in Turkey was investigated using a novel designed primer set for EHV-1. The molecular analysis of TR EHV-1 strains showed that genetically identical TR EHV-1 strains were still circulating in Turkey during different years and, these strains were closely related to the European EHV-1 strains. Furthermore, EHV-1 isolated in the present study and to EHV-1 strains published previously in the GenBank database were shown some differences for the aa sequences. This molecular report would be valuable for monitoring of EHV-1 infection in Turkey and determining the gB gene sequence of newly identified EHV-1 field strains from future outbreaks on the pathogenesis and severity of disease.

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Molecular and pathological investigations of EHV-1 and EHV-4 infections in horses in Turkey

Cited by 15 publications

References 28 publications

Detection and molecular characterization of equine herpesviruses 1, 2, and 5 in horses in the Republic of Serbia

Detection and molecular characterization of equine herpesviruses 1, 2, and 5 in horses in the Republic of Serbia

First Report on the Frequency and Molecular Detection of Neuropathogenic EHV-1 in Turkey

Partial sequence of the gB gene of equid herpesvirus type 1 isolates associated with abortion in Turkey

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