Molecular and Phenotypic Characteristics of CMY-2 .BETA.-Lactamase-Producing Salmonella enterica Serovar Typhimurium Isolated from Cattle in Japan

Abstract

ABSTRACT. Isolates of extended-spectrum cephalosporin (ESC)-resistant Salmonella enterica serovar Typhimurium obtained from two different farms in Fukushima Prefecture, Japan, in 2007 were characterized in order to determine the genetic basis of resistance. ESC resistance in the two isolates was mediated by an AmpC β-lactamase encoded by the bla CMY-2 gene, which is located in a large selftransmissible plasmid in each isolate. The sizes of the bla CMY-2 -carrying plasmids were different. The replicon types of the plasmids were I1-I and A/C. The results of macrorestriction analysis and phage typing suggest a close relationship between both isolates. This is the first report of ESC-resistant S. Typhimurium isolated from cattle in Japan.

Genetic Analysis of Non-Hydrogen Sulfide-Producing Salmonella enterica Serovar Typhimurium and S. enterica Serovar Infantis Isolates in Japan

“…(13), and poultry meat is most frequently contaminated with CMY-2-producing Salmonella (14). Indeed, CMY-2-producing S. Infantis and S. Typhimurium isolates have been found in retail chicken and cattle in Japan (15,16). Because of the clinical importance of third-and fourth-generation cephalosporin in human and veterinary medicine, it is of further concern that insufficient testing might lead to antimicrobial-resistant Salmonella isolates being missed.…”

Plasmid-mediated AmpC β-lactamase (CMY-2) gene in Salmonella typhimurium isolated from diarrheic pigs in South Korea

“…Recently, Salmonella has developed resistance to cephalosporin through the transmission of PABL [12], of which CMY-2 is the most common. CMY-2 was first reported in the USA and is the most widely distributed PABL, with cases also reported in France, Germany, Greece and the United Kingdom; indeed, it was recently isolated from a cow in Japan and from pigs in China [1,3,12-14]. In most cases, the CMY-2 gene is present in large plasmids, of which several genetic types have been reported.…”

Third-Generation Cephalosporin-Resistant Non-Typhoidal <i>Salmonella</i> Isolated from Human Feces in Japan

“…These 2 Inc group plasmids are also associated with the spread of bla CMY and bla CTX-M in food-producing animals. In Japan, IncA/ C, IncI1, and IncP plasmids carrying bla CMY-2 have been identified in Salmonella isolates from cattle and broilers (14,15). Though limited in number, those Inc plasmids were detected among NTS isolates harboring bla CMY-2 from food handlers in addition to diarrheic patients: these findings raise concerns about future increases in third-generation cephalosporin-resistance among invasive NTS, causing an important clinical and public health issue.…”