Molecular and phenotypic characterization of Photobacterium damselae among some marine fishes in Lake Temsah

Eissa, Ismail; Ismail, M. A.; El-Lamie, Maather; Dessouki, Amina; Elsheshtawy, Hassnaa Mahmoud; Bayoumy, Elsayed M.

doi:10.1016/j.micpath.2017.12.006

Cited by 20 publications

(18 citation statements)

References 33 publications

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“…The observed pathology of cultured Nile tilapia infected with P. damselae has some resemblance to that of other marine finfish infected with Phdd , which showed typical haemorrhagic septicaemia, accompanied by external ulcerations (Rivas et al., 2013). Similar characteristic lesions have been detected in cultured sea bream and silver pomfret infected with Phdd (Eissa et al., 2018; Labella et al., 2011; Tao et al., 2018). Our isolates were Gram‐negative with bipolar staining, cytochrome oxidase and catalase positive.…”

Section: Discussionsupporting

confidence: 66%

“…These phenotypic characteristics of P. damselae isolates in Nile tilapia corresponded with the description of major marine fish species infected by P. damselae subsp. damselae (Eissa et al., 2018; Essam et al., 2016; Tao et al., 2018), and a similar trend was reported in Indian broodstock of Pacific white leg shrimp Litopenaeus vannamei (Singaravel et al., 2020). Furthermore, bacterial isolates of Nile tilapia were unambiguously assigned to be P. damselae subsp.…”

Section: Discussionsupporting

confidence: 55%

“…piscicida (Labella et al., 2017; Osorio, Collins, Toranzo, Barja, & Romalde, 1999). In particular, this emerging pathogen was increasingly isolated from disease outbreaks in sea bass, gilthead sea bream, Mugil capito and Nile tilapia, and it was increasingly isolated from disease outbreaks in sea bass, gilthead sea bream, Mugil capito and Nile tilapia reared in the Egyptian coastal provinces with mass mortalities when outbreaks occurred (Abdel‐Aziz et al., 2013; Eissa et al., 2018; Elbahnaswy & Elshopakey, 2020; Essam, Abdellrazeq, Tayel, Torky, & Fadel, 2016; Khalil & Mesalhy, 2008). In the current study, P. damselae has been isolated from epizootic outbreaks affecting cultured Nile tilapia in hot season at the end of June in Lake Manzala, Egypt.…”

Section: Discussionmentioning

confidence: 99%

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Molecular and histopathological characterization of Photobacterium damselae in naturally and experimentally infected Nile tilapia (Oreochromis niloticus)

El‐Son

Elbahnaswy

Ibrahim

2020

Journal of Fish Diseases

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Mass mortality has occurred among cultured Nile tilapia, Oreochromis niloticus, on fish farms in Manzala, Dakahlia province, Egypt, in the summer season, 2019. Moribund fish were reported with deep ulcers, septicaemic lesions and sampled for bacterial isolation. In this study, most isolates were subjected to bacteriological examination, antibiotic sensitivity test, 16S rRNA gene sequencing and histopathological examination. Following isolate identification, intraperitoneal challenge of Nile tilapia with a bacterial suspension 2 × 106 CFU/ml was performed. Samples from liver, spleen and kidney were collected for histological and biochemical analysis. The results showed a high similarity (99%) to Photobacterium damselae strains using phylogenetic analysis of 16S rRNA. P. damselae exhibited resistance to amoxicillin and erythromycin, as well it was highly sensitive to chloramphenicol and doxycycline. Moreover, haemorrhage, oedema, hemosiderosis and melanomacrophage activation in the liver and head kidney of infected fish were detected by light and electron microscopy. Also, significant higher levels of CAT and SOD in the spleen and head kidney, as well as the serum levels of NO were observed in experimentally challenged O. niloticus, compared to the control fish. Our data identified P. damselae for the first time from infected Nile tilapia, describing its sensitivity to a variety of antibiotics, histopathological alterations and oxidative stress impact, and it could be useful indicators for understanding P. damselae pathogenesis, which might provide a preventive efficacy for P. damselae.

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Section: Discussionsupporting

confidence: 66%

Section: Discussionsupporting

confidence: 55%

Section: Discussionmentioning

confidence: 99%

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Molecular and histopathological characterization of Photobacterium damselae in naturally and experimentally infected Nile tilapia (Oreochromis niloticus)

El‐Son

Elbahnaswy

Ibrahim

2020

Journal of Fish Diseases

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“…Family Vibrionaceae and genus Photobacterium were more abundant in high replacement level groups (FS50, FS75 and FS100) than those in low replacement level groups (FM and FS25). It is well known that family Vibrionaceae and genus Photobacterium contain lots of notorious pathogens causing huge F I G U R E 2 Venn diagram of shared and specific OTUs among five groups F I G U R E 3 Relative abundance of bacterial phyla in the five dietary groups diseases and economic losses in marine animals (Cano-Gomez, Bourne, Hall, Owens, & Høj, 2009;Eissa et al, 2018;Su, Lin, & Chen, 2018). Previous studies on intestinal microbiota of shrimp also reported that the population of Vibrionales became predominant in diseased shrimp (Zhu et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Litopenaeus vannamei fed diets with different replacement levels of fish meal by fish silage: A molecular approach on intestinal microbiota

2019

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We investigated the intestinal microbiota in shrimp fed diets with five replacement levels (0, 25%, 50%, 75% and 100%) of fish meal by fish silage. An 8‐week feeding trail was conducted in 15 fibreglass tanks (400 L volume, three replications for each diet) with 50 shrimps (initial weight 0.26 ± 0.03 g) per tank. After 8‐week trial, shrimp fed diets with low replacement levels (0% and 25%) of fish meal by fish silage had significantly enhanced growth performance (p < 0.05). Based on the Illumina sequencing of 15 samples, a total of 5,778 OTUs were obtained and the number of OTUs in each sample ranged from 2,346 to 3,491. OTUs were identified into 23 phyla, and Proteobacteria was the most abundant. At family and genus levels, family Vibrionaceae and genus Photobacterium were more abundant in high replacement level groups (50%, 75% and 100%) than those in low replacement level groups (0% and 25%), which may be related to the worse growth performance of high replacement level groups. However, dietary different levels of fish silage did not make significant difference on intestinal bacterial richness, diversity and metabolism functions (p > 0.05), which indicated the limited impact of dietary fish silage on intestinal microbiota.

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“…It has been reported as a primary pathogen causing diseases in turbot ( Scophthalmus maximus ) ( Fouz et al, 1992 ), sea bream ( Sparus aurata ) ( Vera et al, 1991 ) and other sparid fish species ( Company et al, 1999 ; Labella et al, 2006 ), sea bass ( Dicentrarchus labrax ) ( Abdel-Aziz et al, 2013 ; Uzun and Ogut, 2015 ), rainbow trout ( Oncorhynchus mykiss ) ( Pedersen et al, 1997 , 2009 ), shrimp ( Exopalaemon carinicauda ) ( Liu et al, 2016 ), and etc. The geographical distribution of this bacterium is increasing and nowadays it constitutes an emerging pathogen in aquaculture ( Abdel-Aziz et al, 2013 ; Khouadja et al, 2014 ; Terceti et al, 2016 ; Sharma et al, 2017 ; Eissa et al, 2018 ; Tao et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Molecular Epidemiology of Photobacterium damselae subsp. damselae Outbreaks in Marine Rainbow Trout Farms Reveals Extensive Horizontal Gene Transfer and High Genetic Diversity

et al. 2018

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The marine bacterium Photobacterium damselae subsp. damselae is a pathogen for a variety of marine animals, as well as for humans, and is nowadays considered an emerging pathogen for fish of importance in marine aquaculture. Recent studies have suggested that outbreaks in fish farms are caused by multiclonal populations of this subspecies that exist in the environment. Here, we report the study of a collection of 31 strains isolated during the course of disease outbreaks in marine rainbow trout farms in Denmark in 1994, 1995, and 2006, respectively. A phylogenetic analysis based on the toxR gene sequence, and the screening of virulence-related genes uncovered a high genetic heterogeneity, even among strains isolated from the same fish farm at the same time. Moreover, comparative analysis of the whole genome sequences of four selected strains revealed a large number of differentially occurring genes, which included virulence genes, pPHDD1 plasmid, polysaccharide synthesis gene clusters, CRISPR-Cas systems and putative new mobile genetic elements. This study provides sound evidence that P. damselae subsp. damselae outbreaks in Danish rainbow trout farms were caused by multiclonal populations and that horizontal gene transfer constitutes a strong driving force in the generation of intraspecific diversity in this pathogen.

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Molecular and phenotypic characterization of Photobacterium damselae among some marine fishes in Lake Temsah

Cited by 20 publications

References 33 publications

Molecular and histopathological characterization of Photobacterium damselae in naturally and experimentally infected Nile tilapia (Oreochromis niloticus)

Molecular and histopathological characterization of Photobacterium damselae in naturally and experimentally infected Nile tilapia (Oreochromis niloticus)

Litopenaeus vannamei fed diets with different replacement levels of fish meal by fish silage: A molecular approach on intestinal microbiota

Molecular Epidemiology of Photobacterium damselae subsp. damselae Outbreaks in Marine Rainbow Trout Farms Reveals Extensive Horizontal Gene Transfer and High Genetic Diversity

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