Molecular and Phenotypical Characterization of &amp;lt;i&amp;gt;Mannheimia haemolytica&amp;lt;/i&amp;gt; Isolated from Goats in Baghdad Province

Ahmed, Waffa A.; Mohammed, Roua J.; Khalaf, Ilham A.

doi:10.4236/aim.2017.74025

Cited by 8 publications

(6 citation statements)

References 15 publications

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“…Finding of this study is in agreement with [35] who found results of one hundred nasopharyngeal swabs of apparently healthy eld goats (0.00%) were negative for M. haemolytica and stated that seasonal variation is one factor for the agent, in Baghdad, Iraq. Predisposing factors such as stress in the region's current climatic conditions and changing weather patterns may have led to stress formation in the form of natural incidences of pneumonic pasteurollosis caused by M.haemolytica [42].…”

Section: Discussionsupporting

confidence: 91%

“…The isolate of M.haemolytica was found to be Gram-negative, coccobacilli, non-motile, bipolar, non-sporeforming, catalase and oxidase positive. These ndings were consistent with [35]. Biochemically isolates fermented mannitol, arabinose and glucose while negative for indole, citrate, urease, and, trehalose this nding in line with observations of (Legesse et al, 2018).…”

Section: Discussionsupporting

confidence: 88%

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MALDI TOF Mass Spectrometry and Molecular detection of Mannheimia haemolytica from sheep and goats in Holeta and Sebeta Town, Oromia Special Zone, Ethiopia

Umer,

Bulto,

Bekele

et al. 2023

Preprint

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Background:Mannhemia haemolytica is one of the most important bacteria among causative agent of pneumonic pasteurellosis in small ruminants throughout worldwide and it is also economically devastating pathogen in Ethiopia. Methods: A cross sectional study was carried out from November 2021 to May 2022 with the aim to identify Mannheimia haemolytica from sheep and goat in Sebeta and Holeta town, Oromia special zone, Ethiopia. A total of 235 samples (213 nasal swabs and 22 whole blood) were collected. Sheep and goat with clinical signs suggestive of pneumonic pasteurollosis were purposively sampling. Bacterial identification was conducted using biochemical, Biolog, Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI TOF MS) and Real time PCR detection. Moreover, antimicrobials susceptibility test was also conducted on the identified bacterial isolates using disc diffusion method. Results: The result showed that from a total of 235 samples, only two nasal swab samples were positive for M. haemolytica (0.85%). The two isolates were confirmed by all the tests and similar result was obtained by; biochemical, Biolog, MALDI TOF MS and real time PCR. Up on antimicrobial susceptibility testing, the two isolates were resistant to Streptomycin, Erythromycin and Clindamycin whereas they were susceptible to Tetracycline, Chloramphenicol, Trimethoprim/sulfonamides and Penicillin. Generally, this study revealed that M. haemolytica is among the causative agent of pneumonic pasteurellosis in sheep and goat in the study area. Although, the other remaining bacteria responsible for the disease. Conclusion: The research suggests that a combination of diagnostic methods such as MALDI TOF MS, Biolog, and real-time PCR should be used, as well as for a more in-depth investigation to identify the strain or serotype of M. haemolytica using advanced molecular sequencing and also analysis of the remaining causal agent from various species and locations in countries are significant to address the present vaccination and antibiotic resistance issues.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 88%

MALDI TOF Mass Spectrometry and Molecular detection of Mannheimia haemolytica from sheep and goats in Holeta and Sebeta Town, Oromia Special Zone, Ethiopia

Umer,

Bulto,

Bekele

et al. 2023

Preprint

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“…Other authors have also obtained nonhemolytic M. haemolytica isolates and confirmed them using molecular methods (YENER et al, 2009;MAHU et al, 2015). High sensitivity and specificity of the test were observed in hemolytic as well as non-hemolytic isolates, which makes the molecular test indispensable and recommended for definitive diagnosis of M. haemolytica pneumonia (RAHAL et al, 2014;AHMED et al, 2017).…”

Section: Discussionmentioning

confidence: 94%

“…The disease can cause high economic losses due to treatment costs, slowdown in weight gain, condemnation of carcasses, and a decrease of meat and milk production (EMIKPE et al, 2010;PONNUSAMY et al, 2017). Diagnosis is based on clinical history, clinical signs, gross and histopathological findings, associated with bacteria isolation, and confirmation by molecular techniques (AHMED et al, 2017;EKONG et al, 2015;PONNUSAMY et al, 2017;RICE et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Mannheimia haemolytica pleuropneumonia in goats associated with shipping stress

et al. 2019

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This study described the epidemiological, clinical, pathological and bacteriological aspects of Mannheimia haemolytica pleuropneumonia in goats associated with shipping stress. Forty goats transported from the Northeast to the Southern region of Brazil died during shipment, or 2-3 days after unloading. Clinical signs included dyspnea, mucopurulent nasal discharge, and coughing. All goats were necropsied, and multiple tissues were collected for histopathological analysis and involved agent identifications. All lungs showed pulmonary consolidation, predominantly affecting the cranioventral lobes, in addition to the marked fibrinous pleuritis, pleural thickening, and pleural adhesions, that affected 90% of the goats. Histologically, there was fibrino-suppurative pleuropneumonia characterized by a diffuse neutrophilic infiltrate admixed with fibrin. Non-hemolytic [85% (34/40)] and hemolytic [15% (6/40)] species were obtained in bacteriological culture. Fir Mannheimia spp. was reported in 26 isolates and subsequently confirmed as M. haemolytica (99% identity), after amplification and partial sequencing of the 16S rDNA gene. Stress may trigger the development of bacterial pleuropneumonia in goats, and non-hemolytic strains of M. haemolytica may cause this condition in goats with severe immunosuppression.

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“…Pure cultures of single colony type from MacConkey agar were transferred onto nutrient agar for a series of primary and secondary biochemical tests for bacterial identification as described elsewhere. 9 , 28 …”

Section: Methodsmentioning

confidence: 99%

A Preliminary Investigation on a Commercial Ovine Pasteurellosis Vaccine Using Clinical and Pathological Endpoints

Asfaw¹,

Senbit²,

Yesuf³

et al. 2022

IDR

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Introduction In this study we aimed to provide preliminary evidence on the safety and efficacy of the currently used ovine pasteurellosis vaccine in Ethiopia using clinical and pathological endpoints. Methods Twenty, conventionally reared, apparently healthy, seronegative male lambs, were randomly classified into two groups of 10 animals as “vaccinated-challenged” and ”unvaccinated-challenged controls”. The first group received 1 mL of the licensed Pasteurella multocida biotype A based vaccine subcutaneously while the second group received phosphate-buffered saline as a placebo. Following vaccination, lambs were monitored for one month for potential vaccine adverse reactions. Five weeks postvaccination, all lambs were immunosuppressed using dexamethasone, and intratracheally challenged with 5.2×10 9 CFU/mL live Mannheimia haemolytica A1 (clinical isolates). Then, all lambs were followed up for eight days for clinical examination and necropsied on the ninth day postchallenge for pathological investigation. Results There were no safety issues recorded during the study. In terms of clinical signs, lambs developed fever, depression, mucoid bilateral oculonasal discharge, coughing and sneezing regardless of their vaccination status. Fisher’s exact test between vaccination status and each clinical sign showed a statistically insignificant association ( p >0.05). The main pathological findings in both groups were pulmonary congestion, atelectasis, emphysema, and suppurative bronchopneumonia. Consolidation lung lesion score of +1 (5/10 of vaccinated, 6/10 of unvaccinated) and +2 (3/10 of vaccinated, 4/10 of unvaccinated) were recorded in a statistically indifferent manner among both vaccinated and nonvaccinated groups ( p >0.05). Discussion and Conclusion Collectively, the results suggested that the vaccine posed no safety concern and presumably lacks protective efficacy against local isolates. However, the study did not analyze antibody titer and their functionality using serum bactericidal assays. Further confirmatory studies could provide more evidence on the vaccine efficacy. Safety should further be assessed in a field setting involving a large number of animals to enable detection of rare vaccine adverse events.

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Molecular and Phenotypical Characterization of <i>Mannheimia haemolytica</i> Isolated from Goats in Baghdad Province

Cited by 8 publications

References 15 publications

MALDI TOF Mass Spectrometry and Molecular detection of Mannheimia haemolytica from sheep and goats in Holeta and Sebeta Town, Oromia Special Zone, Ethiopia

MALDI TOF Mass Spectrometry and Molecular detection of Mannheimia haemolytica from sheep and goats in Holeta and Sebeta Town, Oromia Special Zone, Ethiopia

Mannheimia haemolytica pleuropneumonia in goats associated with shipping stress

A Preliminary Investigation on a Commercial Ovine Pasteurellosis Vaccine Using Clinical and Pathological Endpoints

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