Molecular and phylogenetic characterization of multidrug resistant extended spectrum beta-lactamase producing Escherichia coli isolated from poultry and cattle in Odisha, India

Kar, Debasish; Bandyopadhyay, Samiran; Bhattacharyya, Debasish; Samanta, Indranil; Mahanti, Achintya; Nanda, Pramod Kumar; Mondal, Bimalendu; Dandapat, Premanshu; Das, Anubrata; Dutta, Tapan Kumar; Bandyopadhyay, S.; Singh, Raj Kumar

doi:10.1016/j.meegid.2014.11.003

Cited by 97 publications

(98 citation statements)

References 29 publications

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“…Our results revealed that CTX-M, mainly the CTX-M-15, was the most prevalent genotype, followed by TEM and SHV. These findings agree with other contemporary studies from China and around the world that also reported CTX-M as the dominant ESBL genotype (Locatelli et al, 2009; Dahmen et al, 2013; Geser et al, 2015; Kar et al, 2015). This goes along with the recent detection of CTX-M-15 producing E. coli from cattle and other food-animals in east Asia (Ohnishi et al, 2013; Yu et al, 2015), India (Upadhyay et al, 2015), the United Kingdom (Timofte et al, 2014), Germany (Freitag et al, 2016) and Tanzania (Seni et al, 2016).…”

Section: Discussionsupporting

confidence: 92%

“…In addition, low susceptibility was also observed against the common β-lactam and non-β-lactam antibiotics such as ampicillin, aminoglycosides, tetracycline and fluoroquinolones. Recently, many studies have reported MDR ESBL-producing E. coli isolated from cattle (Timofte et al, 2014), poultry (Kar et al, 2015), pigs (Xu et al, 2015), and humans (Gu et al, 2008). Fluoroquinolones, following ciprofloxacin, are the second important antimicrobial drug in veterinary and human medicine (Coque et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

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ESBL-Producing Escherichia coli from Cows Suffering Mastitis in China Contain Clinical Class 1 Integrons with CTX-M Linked to ISCR1

et al. 2016

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The prevalence of pathogenic multi-drug resistant (MDR) extended-spectrum β-lactamase (ESBL)-producing Escherichia coli is rapidly increasing, becoming a global concern. In a veterinary context, ESBL-producing E. coli are mostly reported in poultry and pigs. Here, we report on the prevalence and characterize ESBL-producing E. coli isolated from diverse dairy farms in China. Overall, 36 (23.53%) out of 153 E. coli isolates from mastitic milk samples (n = 1252) were confirmed as ESBL-producers by double-disc synergy testing and PCR. Nucleotide analysis of PCR amplicons revealed that blaCTX-M was the predominant ESBL gene detected in 28 (77.78%) isolates, with blaCTX-M-15 being the major (78.57%) allele encoding for ESBLs. Also, 20 (55.56%) and 6 (16.67%) of the ESBL isolates were carrying blaTEM and blaSHV genes, respectively, in singlet or in combination. The majority of these isolates belonged to phylo-group A (69.44%) and D (16.67%). Strikingly, all these isolates were found to be MDR showing high resistance to cephalosporins including the fourth generation cefepime and common non β-lactams. Additionally, class 1 integrons (intI1) were found in 30 (83.33%) isolates. Analysis of the class 1 integrons variable regions indicated that they were carrying up to five different gene cassettes conferring resistance to various drugs with a predominant combination of dfrA17-aadA5 genes in tandem, conferring resistance to aminoglycosides and trimethoprim. However, no ESBL encoding genes were found in the cassettes. Interestingly, 22 (66.11%) of the ESBL isolates were also carrying insertion sequence common region 1 (ISCR1) which was found to be associated with most of the CTX-M genes. Altogether, the current study reports on the high prevalence of ESBL-positive E. coli, particularly CTX-M-15, carrying clinical class 1 integrons and ISCR1 elements are likely indicative of their rapid and wider dissemination, posing threats to veterinary and public health. To the best of our knowledge, this is the first comprehensive study to report on the alarming high occurrence of ESBL-producing E. coli from mastitic cows in China.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

ESBL-Producing Escherichia coli from Cows Suffering Mastitis in China Contain Clinical Class 1 Integrons with CTX-M Linked to ISCR1

et al. 2016

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“…Prior studies in broilers in West Bengal revealed a higher occurrence rate (29.4%) of eSBL-E. coli (SaMaNta et al, 2015a). however, in other states in India, such as in Odisha and in Mizoram, the occurrence of eSBL-E. coli in broilers was low (3.9%-6.3%) (LaLZaMPUIa et al, 2014;kar et al, 2015). Comparison of extended-spectrum cephalosporinase producing-E. coli occurrence with other studies is difficult due to differences in sample size, the methodology used in the laboratory, and geographical location.…”

Section: Discussionmentioning

confidence: 90%

“…the birds are successfully reared in a backyard system because they can easily scavenge food like indigenous birds (ahUJa et al, 2008). In India, earlier studies conducted P. Ghosh et al: extended-spectrum cephalosporinase possessing E. coli in kuroilers in healthy livestock, such as cattle, pigs and broilers, revealed the existence of eSBLproducing E. coli and Klebsiella pneumoniae (LaLZaMPUIa et al, 2013;LaLZaMPUIa et al, 2014;SaMaNta et al, 2015;SaMaNta et al, 2015a;kar et al, 2015;MaNDakINI et al, 2015). the status of other domestic birds such as kuroilers as reservoirs of extendedspectrum cephalosporinase-producing E. coli is still unexplored.…”

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confidence: 99%

Occurrence of extended-spectrum cephalosporinase producing Escherichia coli in kuroiler birds

et al. 2017

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the present study was undertaken to detect the incidence of extended-spectrum-cephalosporinase gene possessing-Escherichia coli, its co-resistance pattern against other antimicrobials, and the clonal relationship of the isolates in healthy kuroiler birds. a total of 80 cloacal swabs from kuroilers were collected randomly from West Bengal, India. the use of costly antimicrobials (cephalosporins) was not practiced by farmers. Escherichia coli was isolated and identified by standard biochemical tests and 16SrRNA-PCR. All the E. coli isolates, including controls, were subjected to PCr for detection of bla CTX-M , bla TEM, bla SHV, and bla CMY-2 genes. By comparing the raPD-banding pattern, the phylogenetic relationship among the isolates was established. all the isolates were tested for phenotypical resistance against other antibiotics. In total, 60 isolates were identified as E. coli from the kuroilers studied (n = 80). among them, 12 (20%) isolates possessed one of the studied extended-spectrum cephalosporinase genes. among the studied genes, bla TEM and bla SHV were detected in 6 (10%) and 12 (20%) E. coli isolates, respectively. None of the E. coli isolates possessed bla CTX-M and bla In phylogenetic analysis, the strains isolated from same localities with similar genetic profile were grouped into the same cluster. resistance of extended-spectrum cephalosporinase gene possessing E. coli isolates was observed most frequently against ampicillin/cloxacillin, co-trimoxazole, amoxyclav, piperacillin, ceftriaxone, and tetracycline. Kuroiler birds with no cephalosporin usage profile may act as a reservoir of extended-spectrum cephalosporinase gene possessing E. coli. This is the first systematic study in kuroilers, to raise the awareness of consumers regarding the possibility of transmission of antimicrobial resistant E. coli from them.

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“…By contrast, 100 % genetic similarity between strains from pig manure and upstream water was not found. The result indicated that ESBLproducing E. coli isolates from animal farms may pollute the surrounding aquatic environments via sewage discharge (Korzeniewska et al 2013;Kar et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Spread of extended-spectrum beta-lactamase-producing Escherichia coli from a swine farm to the receiving river

Wang

Song

Zhou

et al. 2015

Environ Sci Pollut Res

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The dissemination of drug-resistant bacteria into different environments has posed a grave threat to public health, but data on the spread of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli (E. coli) from animal farms to the receiving river are limited. Here, 57 ESBL-producing E. coli isolated from a pig farm and the receiving river were analyzed in terms of drug resistance, ESBL genes, and enterobacterial repetitive intergenic consensus (ERIC). The results showed that ESBL-producing E. coli from swine feces and downstream water of the pig farm outfall overlapped substantially in drug resistance and ESBL genes. Additionally, six ESBL-producing E. coli from the downstream water exhibited 100 % genetic similarity with strains from the swine feces. In conclusion, effluents of animal farms are a likely contributor to the presence of ESBL-producing E. coli in aquatic environments.

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Molecular and phylogenetic characterization of multidrug resistant extended spectrum beta-lactamase producing Escherichia coli isolated from poultry and cattle in Odisha, India

Cited by 97 publications

References 29 publications

ESBL-Producing Escherichia coli from Cows Suffering Mastitis in China Contain Clinical Class 1 Integrons with CTX-M Linked to ISCR1

ESBL-Producing Escherichia coli from Cows Suffering Mastitis in China Contain Clinical Class 1 Integrons with CTX-M Linked to ISCR1

Occurrence of extended-spectrum cephalosporinase producing Escherichia coli in kuroiler birds

Spread of extended-spectrum beta-lactamase-producing Escherichia coli from a swine farm to the receiving river

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