Spread of extended-spectrum beta-lactamase-producing Escherichia coli from a swine farm to the receiving river

Wang, Jintao; Song, Wengang; Zhou, Yufa; Tang, Yujing; Gao, Yanxia; Miao, Zengmin

doi:10.1007/s11356-015-4575-7

Cited by 32 publications

(9 citation statements)

References 27 publications

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“…The total volume of PCR reaction was 25 μ L, including 10x buffer 2.5 μ L, 2.5 mmol/L dNTP 2.0 μ L, forward and reverse primers (20 pmol/L) 1 μ L for each, 25 mmol/L MgCl 2 1.5 μ L, Taq polymerase 5 U, and genome DNA 1.5 μ L. The ERIC-PCR was completed by an initial heat activation of 10 min at 94°C, then 31 cycles of 30 s at 92°C and 60 s at 40°C, 8 min at 65°C, and an extension of 8 min at 65°C. PCR products were run on an agarose gel electrophoresis (2%) at 100 V for 1 h [ 15 ].…”

Section: Methodsmentioning

confidence: 99%

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Prevalence and Characteristics ofSalmonellaIsolated from Free-Range Chickens in Shandong Province, China

Zhao

Gao

et al. 2016

BioMed Research International

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Compared with chickens raised in intensively managed breeding farms, free-range chickens in China are quite popular due to lower breeding density and less antibiotics usage. However, investigations about Salmonella enterica from free-range chickens are quite rare. The aim of the present study was to investigate prevalence and characteristics of Salmonella in free-range chickens in Shandong province, China. During the period of August and November 2015, 300 fresh fecal swabs from different broilers in three free-range chicken farms (100 samples per farm) were collected to isolate Salmonella, and then these isolates were subjected to serotyping, antibiotic sensitivity testing, enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR), and multilocus sequence typing (ST). A total of 38 Salmonella isolates (38/300, 12.7%) were recovered. The most common serotype was Enteritidis (81.6%), followed by Indiana (13.2%) and Typhimurium (5.3%). Twenty-two out of 38 isolates (57.9%) were resistant to ampicillin, the highest resistance rate, but resistance rates to cefazolin, cefotaxime, and ceftazidime were only 7.9%. The multidrug resistance (MDR) rate was 26.3%. Additionally, the Salmonella isolates could be classified into 25 genotypes by ERIC-PCR and were divided into three ST types (ST11, ST17, and ST19), with ST11 the highest isolation rate (81.6%). In summary, as with other poultry, free-ranging chickens may also serve as potential reservoir for antibiotic resistant Salmonella, thereby posing a threat to public health.

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Section: Methodsmentioning

confidence: 99%

“…The unweighted pair group method using averages algorithm (UPGMA) was used to obtain the clustering dendrograms (NTSYS-pc 2.10 software). The individual isolates were regarded as an independent operational taxonomic unit (OUT), and isolates with a similarity over 90% were assumed to be of the same origin [ 15 ].…”

Section: Methodsmentioning

confidence: 99%

Prevalence and Characteristics ofSalmonellaIsolated from Free-Range Chickens in Shandong Province, China

Zhao

Gao

et al. 2016

BioMed Research International

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“…Date of publication ranged from 1995 to 2016, with the highest number of publications in 2015 ( n = 11). The spatial scale for the sampling frame ranged from 40 m (Li, Song, et al, ) to 450 km (Sulzner et al., ); six studies did not report a specific distance.…”

Section: Resultsmentioning

confidence: 99%

“…Li, Atwill, et al. () reported higher proportion of ESBL downstream compared to upstream from a swine farm (Li, Song, et al, ). Sapkota et al.…”

Section: Resultsmentioning

confidence: 99%

Systematic Review: Impact of point sources on antibiotic‐resistant bacteria in the natural environment

Bueno

Williams-Nguyen

Hwang

et al. 2017

Zoonoses and Public Health

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SummaryPoint sources such as wastewater treatment plants and agricultural facilities may have a role in the dissemination of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARG). To analyse the evidence for increases in ARB in the natural environment associated with these point sources of ARB and ARG, we conducted a systematic review. We evaluated 5,247 records retrieved through database searches, including both studies that ascertained ARG and ARB outcomes. All studies were subjected to a screening process to assess relevance to the question and methodology to address our review question. A risk of bias assessment was conducted upon the final pool of studies included in the review. This article summarizes the evidence only for those studies with ARB outcomes (n = 47). Thirty-five studies were at high (n = 11) or at unclear (n = 24) risk of bias in the estimation of source effects due to lack of information and/ or failure to control for confounders. Statistical analysis was used in ten studies, of which one assessed the effect of multiple sources using modelling approaches; none reported effect measures. Most studies reported higher ARB prevalence or concentration downstream/near the source. However, this evidence was primarily descriptive and it could not be concluded that there is a clear impact of point sources on increases in ARB in the environment. To quantify increases in ARB in the environment due to specific point sources, there is a need for studies that stress study design, control of biases and analytical tools to provide effect measure estimates. K E Y W O R D Santibiotic-resistant bacteria, effect measure, environmental dissemination, point source, systematic review

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“…Furthermore, the ESBL/AmpC-producing E. coli in animal farms could influence public health through environmental pollution (Li et al, 2015). Fecal emission and the airborne route might be possible ways of ESBL/AmpC emission into the environment (Yuan et al, 2010; Friese et al, 2013; Laube et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

The Efficacy of Isolated Bacteriophages from Pig Farms against ESBL/AmpC-Producing Escherichia coli from Pig and Turkey Farms

et al. 2017

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Extended-spectrum β-lactamases (ESBLs) and AmpC β-lactamases are plasmid (but also chromosomally) encoded enzymes found in Enterobacteriaceae, determining resistance to a variety of important antibiotics including penicillins, cephalosporins, and monobactams. In recent decades, the prevalence of ESBL/AmpC-producing bacteria has increased rapidly across the world. Here, we evaluate the potential use of bacteriophages in terms of a reduction of antibiotic-resistant bacteria in healthy animals. The aim of our studies was to isolate bacteriophages capable of destroying ESBL/AmpC-producing Escherichia coli isolated from livestock habitats. The efficacy of isolated phages against ESBL/AmpC E. coli strains varies, but creation of a phage cocktail with broad activity spectrum is possible. This may indicate that the role of phages may not be limited to phage therapy, but bacterial viruses may also be applied against spread of bacteria with antibiotic resistance genes in the environment. We also addressed the hypothesis, that phages, effective for therapeutic purposes may be isolated from distant places and even from different environments other than the actual location of the targeted bacteria. This may be beneficial for practical purposes, as the construction of effective phage preparations does not require access to disease outbreaks.

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Spread of extended-spectrum beta-lactamase-producing Escherichia coli from a swine farm to the receiving river

Cited by 32 publications

References 27 publications

Prevalence and Characteristics ofSalmonellaIsolated from Free-Range Chickens in Shandong Province, China

Prevalence and Characteristics ofSalmonellaIsolated from Free-Range Chickens in Shandong Province, China

Systematic Review: Impact of point sources on antibiotic‐resistant bacteria in the natural environment

The Efficacy of Isolated Bacteriophages from Pig Farms against ESBL/AmpC-Producing Escherichia coli from Pig and Turkey Farms

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