Molecular and physiological characterization of six‐way resistance in an <i>Amaranthus tuberculatus</i> var. <i>rudis</i> biotype from Missouri

Shergill, Lovreet S.; Bish, Mandy D.; Jugulam, Mithila; Bradley, Kevin W.

doi:10.1002/ps.5082

Cited by 37 publications

(51 citation statements)

References 67 publications

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“…A codon deletion (ΔG210) or single point mutations in the PPX2 gene mainly confers resistance to fomesafen in PPO‐resistant A. palmeri populations; however, the possibility of non‐target‐site based mechanisms (cytochrome P450‐mediated and GST‐based) has recently been suggested in the PPO‐resistant A. palmeri population in Arkansas . Similarly, the enhanced metabolism of 2,4‐D via cytochrome P450 monooxygenase has been found to confer 2,4‐D resistance in A. tuberculatus populations from Nebraska and Missouri . Nonetheless, the underlying mechanism(s) conferring multiple resistance to five different herbicide site(s) of action and reduced sensitivity to fomesafen in the MHR A. palmeri population confirmed in this study are still unknown and need to be determined.…”

Section: Discussionmentioning

confidence: 99%

Confirmation of 2,4‐D resistance and identification of multiple resistance in a Kansas Palmer amaranth (Amaranthus palmeri) population

Kumar

Boyer

et al. 2019

Pest Management Science

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BACKGROUND: Amaranthus palmeri S. Wats is among the most problematic annual broadleaf weed species in the USA, including in Kansas. In late summer 2015, seeds of an A. palmeri population (MHR) that had survived field-use rates of 2,4-D were collected from Barton County, KS, USA. The main objectives were to: (i) confirm and characterize 2,4-D resistance in a MHR population; (ii) characterize the resistance profile of the MHR population in relation to a multiple herbicide-susceptible (MHS) population to glyphosate, chlorsulfuron, atrazine, mesotrione, fomesafen; and (iii) determine the effectiveness of alternative POST burndown herbicides for controlling MHR population. RESULTS: The MHR population had 3.2-fold resistance to 2,4-D. In addition, the MHR population also exhibited multiple resistance to glyphosate (11.8-fold), chlorsulfuron (5.0-fold), atrazine (14.4-fold), and mesotrione (13.4-fold). Furthermore, the MHR population also showed reduced sensitivity to fomesafen (2.3-fold). In a separate study, dicamba with glyphosate, atrazine or fluroxypyr + 2,4-D, and paraquat alone or with atrazine, metribuzin, saflufenacil or 2,4-D provided ≥ 99% injury to the MHR population. Similarly, saflufenacil alone or with atrazine, metribuzin or 2,4-D, and glufosinate alone or with glyphosate + 2,4-D, and glyphosate + dicamba, and a premix of bicyclopyrone + atrazine + mesotrione + S-metolachlor also effectively controlled the MHR population. CONCLUSION: This research confirms the first global case of an A. palmeri population from Kansas with multiple resistance to 2,4-D, glyphosate, chlorsulfuron, atrazine and mesotrione, and reduced sensitivity to fomesafen. Dicamba, glufosinate, paraquat, and saflufenacil alone or in tank-mixtures with PRE herbicides effectively controlled this MHR population.

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Section: Discussionmentioning

confidence: 99%

Confirmation of 2,4‐D resistance and identification of multiple resistance in a Kansas Palmer amaranth (Amaranthus palmeri) population

Kumar

Boyer

et al. 2019

Pest Management Science

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“…Resistance mechanism characterization should not be ignored, since implementing weed management measures without characterizing them may aggravate resistance from monogenic to polygenic, as reported in A. tuberculatus var. rudis , Epilobium ciliatum , and Kochia scoparia that selected resistance for six, five, and four modes-of-action of herbicides, respectively [21,22,23]. Mechanisms conferring glyphosate resistance were characterized in A. palmeri , A. spinosus and A. tuberculatus [10,24,25]; but there are no studies for A. hybridus .…”

Section: Introductionmentioning

confidence: 99%

The Triple Amino Acid Substitution TAP-IVS in the EPSPS Gene Confers High Glyphosate Resistance to the Superweed Amaranthus hybridus

García

Palma-Bautista

Rojano-Delgado

et al. 2019

IJMS

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The introduction of glyphosate-resistant (GR) crops revolutionized weed management; however, the improper use of this technology has selected for a wide range of weeds resistant to glyphosate, referred to as superweeds. We characterized the high glyphosate resistance level of an Amaranthus hybridus population (GRH)—a superweed collected in a GR-soybean field from Cordoba, Argentina—as well as the resistance mechanisms that govern it in comparison to a susceptible population (GSH). The GRH population was 100.6 times more resistant than the GSH population. Reduced absorption and metabolism of glyphosate, as well as gene duplication of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) or its overexpression did not contribute to this resistance. However, GSH plants translocated at least 10% more 14C-glyphosate to the rest of the plant and roots than GRH plants at 9 h after treatment. In addition, a novel triple amino acid substitution from TAP (wild type, GSH) to IVS (triple mutant, GRH) was identified in the EPSPS gene of the GRH. The nucleotide substitutions consisted of ATA102, GTC103 and TCA106 instead of ACA102, GCG103, and CCA106, respectively. The hydrogen bond distances between Gly-101 and Arg-105 positions increased from 2.89 Å (wild type) to 2.93 Å (triple-mutant) according to the EPSPS structural modeling. These results support that the high level of glyphosate resistance of the GRH A. hybridus population was mainly governed by the triple mutation TAP-IVS found of the EPSPS target site, but the impaired translocation of herbicide also contributed in this resistance.

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“…The coexistence of both target and non-target site resistance mechanisms such as altered ALS gene and enhanced metabolism of ALS-inhibitors in the same weed species has been reported [10,33,34]. Although altered ALS gene is known to confer a high level of resistance [28], enhanced metabolism also can bestow a high level of resistance in some weed species [10,35,36]. The presence of enhanced metabolism or other non-target site mechanisms such as reduced absorption or translocation also need to be tested in the KSW-R wild buckwheat to rule out contribution of non-target resistance mechanism to ALS-inhibitor resistance in this weed.…”

Section: Discussionmentioning

confidence: 99%

Confirmation and Characterization of the First Case of Acetolactate Synthase (ALS)-Inhibitor—Resistant Wild Buckwheat (Polygonum convolvulus L.) in the United States

et al. 2020

Self Cite

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Wild buckwheat (Polygonum convolvulus L.) is a problem weed and ALS-inhibitors (e.g., chlorsulfuron) are commonly used for its management. Recently, a population of wild buckwheat (KSW-R) uncontrolled with ALS-inhibitors was found in a wheat field in Kansas, USA. The objectives of this research were to determine the level and mechanism of resistance to chlorsulfuron and cross resistance to other ALS-inhibitors in the KSW-R population. In response to chlorsulfuron rates ranging from 0 to 16x (x = 18 g ai/ha), the KSW-R wild buckwheat was found >100-fold more resistant compared to a known ALS-inhibitor susceptible (KSW-S) wild buckwheat. Also, >90% of KSW-R plants survived field recommended rates of sulfonylurea but not imidazolinone family of ALS-inhibitors. A portion of the ALS gene covering all previously reported mutations known to bestow resistance to ALS-inhibitors was sequenced from both KSW-R and KSW-S plants. The Pro-197-Ser substitution that confers resistance to the sulfonylurea herbicides was found in KSW-R plants. Our results support the evolution of high level of chlorsulfuron resistance as a result of a mutation in the ALS-gene in KSW-R buckwheat. This is the first case of resistance to any herbicides in wild buckwheat in the US.

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Molecular and physiological characterization of six‐way resistance in an Amaranthus tuberculatus var. rudis biotype from Missouri

Cited by 37 publications

References 67 publications

Confirmation of 2,4‐D resistance and identification of multiple resistance in a Kansas Palmer amaranth (Amaranthus palmeri) population

Confirmation of 2,4‐D resistance and identification of multiple resistance in a Kansas Palmer amaranth (Amaranthus palmeri) population

The Triple Amino Acid Substitution TAP-IVS in the EPSPS Gene Confers High Glyphosate Resistance to the Superweed Amaranthus hybridus

Confirmation and Characterization of the First Case of Acetolactate Synthase (ALS)-Inhibitor—Resistant Wild Buckwheat (Polygonum convolvulus L.) in the United States

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