Molecular and Physiological Differentiation Between Pathogenic and Nonpathogenic Acanthamoeba

Khan, Naveed Ahmed; Jarroll, Edward L.; Paget, Timothy A.

doi:10.1007/s00284-001-0108-3

Cited by 90 publications

(75 citation statements)

References 21 publications

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“…At least 24 named species have been described within this genus, many of them containing both pathogenic and non pathogenic isolates [11,22]. Pussard and Pons [23] classified the genus into three morphological groups; however, this classification has proven to be unreliable due to alternating cyst appearance.…”

Section: Discussionmentioning

confidence: 99%

“…The high proportion of T4 Acanthamoeba associated with keratitis could be attributed to its greater virulence and wide spreading in the environment with respect to other genotypes [8]. At present, also the T2, T3, T5, T6, and T11 genotypes have been associated with AK [3,[8][9][10][11][12]. The other genotypes have been found related to non-AK infections and/or environmental sources [13].…”

Section: Introductionmentioning

confidence: 99%

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Acanthamoeba T4 and T15 genotypes associated with keratitis infections in Italy

Cave

Monno

Bottalico

et al. 2008

Eur J Clin Microbiol Infect Dis

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Thus far there is little data available concerning Acanthamoeba associated amoebic keratitis (AK) from Italy. In order to understand the incidence of Acanthamoeba in patients with ocular infections and to characterize the isolates at the molecular level, ocular specimens and contact lenses or lens case solutions from 140 patients were analysed by culture and by an 18S rRNA (Rns) genebased PCR method. Nineteen (13.6%) patients showed Acanthamoeba culture positive samples. Eleven out of the 14 genetically characterized isolates were assigned to the T4 genotype. Three isolates, two of them from patients with keratitis responding to specific anti-Acanthamoeba therapy, were identified as belonging to the T15 genotype. This finding represents the first association between the T15 genotype and human amoebic keratitis. PCR amplification of the 18S ribosomal DNA proved to be a sensitive method, potentially able to detect Acanthamoeba without the need of long culture incubation, and thus considerably useful for clinical applications.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Acanthamoeba T4 and T15 genotypes associated with keratitis infections in Italy

Cave

Monno

Bottalico

et al. 2008

Eur J Clin Microbiol Infect Dis

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“…In addition, 0.5 mg/mL penicillin (Sigma-Aldrich, Chemical, France), and 0.5 mg/mL streptomycin (Sigma-Aldrich, Chemical, France) were added (Khan et al, 2002).…”

Section: Methodsmentioning

confidence: 99%

The cultivation of Acanthamoeba using with different axenic and monoxenic media

Eroglu¹,

Evyapan²,

Koltas³

2015

Mid. Black Sea J of Health Sci.

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Objective: Acanthamoeba species are the ubiquities free-living amoebae and can infect humans, causing diseases such as keratitis and encephalitis. Acanthamoeba species are often grown on non-nutrient agar spread with Escherichia coli or peptone-yeast extract-glucose. We investigated the amount of growth of Acanthamoeba in different axenic and monoxenic media.Methods: The non-nutrient agar with Pseudomonas aeuroginosa, Enterobacter aerogenes, Staphylococcus aeurous, Escherichia coli, and Klebsiella pneumoniae were used as monoxenic media. The encystation, mycological peptone-maltose, peptone yeast extract glucose, roswell park memorial institute 1640 and trypticase beef hemoglobine media were used as axenic media.Results: We compared the growth of Acanthamoeba species in different axenic and monoxenic media in this study. In relation to the growth rate, the non-nutrient agar with Pseudomonas aeuroginosa had the highest values achieved among monoxenic media and roswell park memorial institute 1640 media was the highest value among axenic media.Conclusion: In view of the results, we can affirm that these monoxenic media are adequate to grow of Acanthamoeba species. In addition, a classic and basic medium that supports the growth of Acanthamoeba species consists of peptone yeast extract glucose. However, the roswell park memorial institute 1640 media was an excellent commercially available media for the growth of Acanthamoeba and it was able to keep Acanthamoeba by long periods of time.

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“…If during the early stage, inappropriate treatment is instituted, the agent can penetrate deeply into the cornea and other parts of the eye (McClellan et al 1987). Thus, Acanthamoeba can invade tissues and settle in the stroma, causing severe infections that can lead to the enucleation of the ocular globe (Khan et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Susceptibility of Acanthamoeba to multipurpose lens-cleaning solutions

Aguiar

Silveira

Winck

et al. 2013

Acta Parasitologica

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The present study investigated the susceptibility of Acanthamoeba spp. trophozoites to two multipurpose systems for cleaning and maintenance of contact lenses. Three strains of trophozoites from the ATCC (A. castellani T4, A. castellani Neff, and A. polyphaga) and two Acanthamoeba isolates obtained from swimming pools (PT5 and PO1) were placed in monoxenic culture. To test their survival in cleaning solutions for contact lenses, the trophozoites were exposed for 4 and 24 h to two multipurpose solutions (A and B), and were then inoculated into a new monoxenic culture. Amoebic growth on the plates was observed after 72 h of incubation. Trophozoites from all three ATCC strains and one isolate from a swimming pool (PO1) grew in all plates after 4 h of exposure to solutions A and B. After 24 h, the ATCC strains and the PO1 isolate showed growth in most of the plates treated. Only the PT5 isolate showed susceptibility to both solutions over the time intervals tested. The two solutions were not completely effective against most strains and isolates over the time intervals tested. These results are important, since species of Acanthamoeba are widely distributed in the environment and are potential agents of eye pathologies.

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Molecular and Physiological Differentiation Between Pathogenic and Nonpathogenic Acanthamoeba

Cited by 90 publications

References 21 publications

Acanthamoeba T4 and T15 genotypes associated with keratitis infections in Italy

Acanthamoeba T4 and T15 genotypes associated with keratitis infections in Italy

The cultivation of Acanthamoeba using with different axenic and monoxenic media

Susceptibility of Acanthamoeba to multipurpose lens-cleaning solutions

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