Molecular and serological characterization of <i>Riemerella</i> isolates associated with poultry in Australia

Omaleki, Lida; Blackall, P. J.; Bisgaard, Magne; Turni, Conny

doi:10.1080/03079457.2020.1828568

Cited by 8 publications

(6 citation statements)

References 43 publications

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“…Studies have shown that R. anatipestifer spreads via air, tainted water, and feed; affects the digestive and respiratory systems; and causes skin infections after wounds ( Pathanasophon et al, 2002 ). Several published papers have reported R. anatipestifer infections in chickens ( Omaleki et al, 2021 ; Tzora et al, 2021 ), but the disease's effects on poultry are poorly understood.…”

Section: Discussionmentioning

confidence: 99%

“…A clinical case of R. anatipestifer infection in commercial broilers in Greece was reported in 2021 ( Tzora et al, 2021 ). Another two typical cases of R. anatipestifer infections were reported in China and Australia ( Omaleki et al, 2021 ). However, little is known about the pathogen's effects on egg laying in chickens carrying this pathogen.…”

Section: Introductionmentioning

confidence: 89%

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Genomic analysis and experimental pathogenic characterization of Riemerella anatipestifer isolates from chickens in China

Chen,

Li,

Liu

et al. 2024

Poultry Science

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 89%

Genomic analysis and experimental pathogenic characterization of Riemerella anatipestifer isolates from chickens in China

Chen,

Li,

Liu

et al. 2024

Poultry Science

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“…Furthermore, serotyping schemes are widely used based on antibodies raised against certain bacterial components such as capsules, somatic structures or flagella. Autoagglutination, crossreactions and non-typeable bacterial strains are regularly found, altogether limiting the approach [71][72][73][74].…”

Section: Bacteriologymentioning

confidence: 99%

Diagnosing Infectious Diseases in Poultry Requires a Holistic Approach: A Review

Liebhart

Bilić

Grafl

et al. 2023

Poultry

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Controlling infectious diseases is vital for poultry health and diagnostic methods are an indispensable feature to resolve disease etiologies and the impact of infectious agents on the host. Although the basic principles of disease diagnostics have not changed, the spectrum of poultry diseases constantly expanded, with the identification of new pathogens and improved knowledge on epidemiology and disease pathogenesis. In parallel, new technologies have been devised to identify and characterize infectious agents, but classical methods remain crucial, especially the isolation of pathogens and their further characterization in functional assays and studies. This review aims to highlight certain aspects of diagnosing infectious poultry pathogens, from the farm via the diagnostic laboratory and back, in order to close the circle. By this, the current knowledge will be summarized and future developments will be discussed in the context of applied state-of-the-art techniques. Overall, a common challenge is the increasing demand for infrastructure, skills and expertise. Divided into separate chapters, reflecting different disciplines, daily work implies the need to closely link technologies and human expertise in order to improve bird health, the production economy and to implement future intervention strategies for disease prevention.

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“…R. anatipestifer infection has caused significant economic losses in the duck industry worldwide. R. anatipestifer has at least 21 serovars ( 1 ), and the protective efficacy of vaccines has been limited due to the absence of cross-protection between serovars ( 2 ).…”

Section: Introductionmentioning

confidence: 99%

Capsular polysaccharide determines the serotyping of Riemerella anatipestifer

Liu,

Luo,

Yang

et al. 2023

Microbiol Spectr

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Riemerella anatipestifer ( R. anatipestifer ) is a Gram-negative pathogen that causes significant economic losses to the farming industry. The polysaccharides that determine serotype are well understood in other bacteria; they have not yet been fully characterized in R. anatipestifer . In this study, we unexpectedly discovered a strain, RCAD0392, that is capable of cross-agglutination. Whole genome sequencing revealed base mutations disrupting a gene on its capsular polysaccharide synthesis gene cluster. By constructing homologous gene deletion mutants in CH-2, we demonstrated that deletion of this gene leads to altered serological phenotypes. India ink and transmission electron microscopy experiments revealed the disappearance of capsule on the surface of the bacteria, indicating the association of the gene with capsule synthesis. In addition, agar-gel precipitin tests showed that lipopolysaccharide binds to antisera of multiple serotypes, while the capsular polysaccharide only binds to the corresponding antisera. This suggests that capsular polysaccharide is the specific antigen that determines the serotype of R. anatipestifer . IMPORTANCE Riemerella anatipestifer ( R. anatipestifer ) is one of the most important veterinary pathogens with at least 21 serotypes. However, the exact polysaccharide(s) that determine R. anatipestifer serotype is still unknown. This study has provided a preliminary exploration of the relationship between capsular polysaccharides and serotyping in R. anatipestifer and suggests possible directions for further investigation of the genetic basis of serotypes in this bacterium.

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Molecular and serological characterization of Riemerella isolates associated with poultry in Australia

Cited by 8 publications

References 43 publications

Genomic analysis and experimental pathogenic characterization of Riemerella anatipestifer isolates from chickens in China

Genomic analysis and experimental pathogenic characterization of Riemerella anatipestifer isolates from chickens in China

Diagnosing Infectious Diseases in Poultry Requires a Holistic Approach: A Review

Capsular polysaccharide determines the serotyping of Riemerella anatipestifer

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