Molecular and serological prevalence of Babesia bovis and Babesia bigemina in cattle from central region of Syria

Terkawi, Mohamad Alaa; Alhasan, Hend; Huyên, Nguyễn Xuân; Sabagh, Amin; Awier, Karam; Cao, Shinuo; Goo, Youn‐Kyoung; Aboge, Gabriel Oluga; Yokoyama, Naoki; Nishikawa, Yoshifumi; Kalballouz, A. K.; Tabbaa, Darem; Igarashi, Ikuo; Xuan, Xuenan

doi:10.1016/j.vetpar.2011.12.038

Cited by 33 publications

(37 citation statements)

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“…Similar results were observed for A. marginale by Ashuma et al (2013). The results of this paper disagree with Terkawi et al (2012) that found greater positivity of Babesia in adult cattle.…”

Section: Discussioncontrasting

confidence: 55%

Bovine babesiosis and anaplasmosis complex: diagnosis and evaluation of the risk factors from Bahia, Brazil

Amorim

Wenceslau

Carvalho

et al. 2014

Rev. Bras. Parasitol. Vet.

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Bovine babesiosis and anaplasmosis complex: diagnosis and evaluation of the risk factors from Bahia, BrazilComplexo tristeza parasitária bovina: diagnóstico e avaliação dos fatores de risco na Bahia, Brasil AbstractDirect diagnoses were made by using -blood smears and nested PCR (nPCR) tests on 309 blood samples from crossbred dairy cattle in the municipality of Ibicaraí, Bahia. From diagnostic blood smear slides, the observed parasitic frequencies were 31.1% for Anaplasma marginale and 20.4% for Babesia sp. From nPCR diagnoses, they were 63% for A. marginale, 34% for Babesia bigemina and 20.4% for Babesia bovis. There were significant differences (P < 0.01) between the two diagnostic methods (nPCR and blood smear slides). The compliance obtained from the kappa test was 0.41 and 0.48 for A. marginale and Babesia sp., respectively. The tick samples from the six farms analyzed using nPCR were only positive for A. marginale. Evaluation of the risk factors relating to the presence of ticks and the age of the animals showed that there was a significant association (P < 0.01) with the frequency of animals infected with both pathogens. Therefore, under the conditions studied, nPCR proved to be a good tool for diagnosing the agents of the bovine babesiosis and anaplasmosis complex because of its sensitivity and specificity in comparison with blood smears. The municipality of Ibicaraí is an area with endemic prevalence of bovine babesiosis and anaplasmosis confirmed by nPCR and A. marginale is the main agent of the disease.Keywords: Bovine Babesiosis and Anaplasmosis Complex, blood smear, nPCR, risk factors. ResumoRealizou-se o diagnóstico direto por esfregaço sanguíneo e nested PCR (nPCR) em 309 amostras de sangue de bovinos mestiços leiteiros provenientes do município de Ibicaraí, Bahia. A frequência observada no diagnóstico por lâminas de esfregaço sanguíneo foi 31,1% para Anaplasma marginale e 20,4% para Babesia sp. Enquanto que no diagnóstico por nPCR foi 63% para A. marginale, 34% para Babesia bigemina e 20,4% Babesia bovis. Verificaram-se diferenças significativas (P<0,01) na comparação entre os dois métodos de diagnósticos (nPCR e esfregaço sanguíneo). A concordância ao teste KAPPA obtida foi de 0,41 e 0,48 para A. marginale e Babesia sp., respectivamente. As amostras de carrapatos das seis propriedades analisadas por nPCR foram positivas apenas para A. marginale. Na avaliação dos fatores de risco verificou-se que a presença de carrapato e idade dos animais apresentaram associação significativa (P<0,01) com a frequência de animais infectados por ambos os patógenos analisados por nPCR. Portanto, nas condições estudadas, a nPCR revelou-se uma boa ferramenta para diagnóstico dos agentes do complexo tristeza parasitária bovina (TPB) devido a sensibilidade e especificidade, quando comparado ao esfregaço sanguíneo. O município de Ibicaraí

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Section: Discussioncontrasting

confidence: 55%

Bovine babesiosis and anaplasmosis complex: diagnosis and evaluation of the risk factors from Bahia, Brazil

Amorim

Wenceslau

Carvalho

et al. 2014

Rev. Bras. Parasitol. Vet.

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“…Molecular method based on the detection of Babesia DNA in blood by PCR techniques provides reliable results with high sensitivity and specificity. Recently, nested PCR (nPCR) targeting B. bovis spherical body protein 2 (BboSBP2) and B. bigemina rhoptry-associated protein-1a (BbiRAP-1a) have proven to be a powerful tool for epidemiological investigations [1,5,10,11].…”

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confidence: 99%

Epidemiological Survey of <i>Babesia bovis</i> and <i>Babesia bigemina</i> Infections of Cattle in Philippines

Terkawi

Cruz-Flores

et al. 2013

J. Vet. Med. Sci.

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ABSTRACT. A total of 250 blood samples were collected from clinically healthy cattle in five provinces of Philippines. DNA was extracted from the samples and analyzed by nested PCR assays for an epidemiological survey of Babesia bovis and Babesia bigemina infections. Out of the 250 samples, 27 (10.8%) and 16 (6.4%) were positive for B. bovis infection and B. bigemina infection, respectively. Mixed infections were detected in a total of 4 samples (1.6%). Our data provide baseline information regarding the epidemiology of B. bovis and B. bigemina infections in cattle in Philippines, which can be utilized in developing proper strategies for disease control and management.

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“…However, at subclinical and chronic stages, this method is not sensitive enough to detect these agents (TERKAWI et al, 2012). Thus, a variety of serological techniques have been applied to the diagnosis of infection with Babesia spp.…”

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confidence: 99%

“…Because of its practicability and high sensitivity, the indirect ELISA method has been used in seroprevalence studies for these agents and considered promising for the diagnosis of babesiosis and anaplasmosis (MACHADO et al, 1997;TERKAWI et al, 2012). Thus, this diagnostic technique has been used in recent decades and is considered a breakthrough in terms of sensitivity, specificity, standardization and reproducibility (MACHADO et al, 1997 Gurupi).…”

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confidence: 99%

“…Sera from newborn cattle, which had not ingested colostrum and had tested negative in a previous ELISA, were used as negative controls. The cutoff point was calculated based on the results of sera from 30 negative animals assessed through a receiver operating characteristic (ROC) analysis using the statistical software MedCalc (version 11.4; http://www.medcalc.be) (TERKAWI et al, 2012 Antibodies against the agents that cause babesiosis and anaplasmosis in cattle had previously been detected in the five regions of the country (VIDOTTO; MARANA, 1999;SOUZA et al, 2000a;2000b;BARROS et al, 2005), and variations in prevalence may have been caused by climate and/or soil characteristics specific to each location. Temperature and humidity are important factors for the development of the tick R. microplus, a common vector of the pathogens that affects the dynamics of transmission in the analyzed regions.…”

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confidence: 99%

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Serological detection of Anaplasma marginale, Babesia bovis and Babesia bigemina in beef cattle of the northern and central-western regions of Brazil

et al. 2015

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The objective of this study was to assess the occurrence of antibodies against Anaplasma marginale, Babesia bovis and Babesia bigemina in beef cattle of the northern and central-western regions of Brazil. A total of 1703 blood samples were collected from cattle from 100 farms distributed among 37 municipalities in the states of Mato Grosso, Pará and Tocantins. The search for antibodies was conducted using an indirect enzyme-linked immunosorbent assay (ELISA), and antibodies were observed for B. bovis, B. bigemina and A. marginale in cattle from Mato Grosso, Tocantins and Pará at rates of 99%, 90% and 41%; 99%, 70% and 52%; and 97%, 97% and 75%, respectively. The results show that the analyzed regions exhibit enzootic stability for infection with B. bovis and B. bigemina, whereas the same result was not observed with A. marginale. Key words: Anaplasmosis, Babesiosis, beef cattle, Northern Region, Central-Western Region ResumoO objetivo deste estudo foi verificar a ocorrência de anticorpos contra Anaplasma marginale, Babesia bovis e Babesia bigemina em bovinos de corte das regiões Norte e Centro Oeste do Brasil. Foram selecionadas rando micamente 1703 amostras de sangue de bovinos em 100 propriedades rurais distribuídos em 37 municípios dos estados do Mato Grosso, Pará e Tocantins. A pesquisa de anticorpos foi realizada por meio do Ensaio de Imunoadsorção Enzimático (ELISA) Indireto. A ocorrência de anticorpos observada para B. bovis, B. bigemina e A. marginale em bovinos dos estados do Mato Grosso, Tocantins e Pará foi 99%, 90% e 41%; 99%, 70% e 52% e 97%, 97% e 75%, respectivamente. Os resultados mostram que as regiões analisadas apresentam estabilidade enzoótica para a infecção por B. bovis e B. bigemina, não sendo observado o mesmo para Anaplasma marginale.

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Molecular and serological prevalence of Babesia bovis and Babesia bigemina in cattle from central region of Syria

Cited by 33 publications

References 22 publications

Bovine babesiosis and anaplasmosis complex: diagnosis and evaluation of the risk factors from Bahia, Brazil

Bovine babesiosis and anaplasmosis complex: diagnosis and evaluation of the risk factors from Bahia, Brazil

Epidemiological Survey of <i>Babesia bovis</i> and <i>Babesia bigemina</i> Infections of Cattle in Philippines

Serological detection of Anaplasma marginale, Babesia bovis and Babesia bigemina in beef cattle of the northern and central-western regions of Brazil

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