Molecular and spatial analyses reveal new insights on Bois noir epidemiology in Franciacorta vineyards

Quaglino, F.; Passera, Alessandro; Faccincani, Monica; Moussa, Abdelhameed; Pozzebon, Alberto; Sanna, Francesco; Casati, P.; Bianco, P.A.; Mori, Nicola

doi:10.1111/aab.12687

Cited by 14 publications

(25 citation statements)

References 46 publications

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“…P. solani’ strains genotypisation based on this particular gene [ 15 , 16 ]. Molecular characterisation on highly variable genes such as stamp and vmp1 (variable membrane protein), has already been used, not only to access genetic variability, but also to give an insight into the complex epidemiological cycles of these plant pathogenic microorganisms that spread through natural and cultivated vegetation [ 12 , 17 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Multilocus Genotyping of ‘Candidatus Phytoplasma solani’ Associated with Rubbery Taproot Disease of Sugar Beet in the Pannonian Plain

et al. 2021

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Rubbery taproot disease of sugar beet (RTD), associated with ‘Candidatus Phytoplasma solani’, appeared in 2020 on an epidemic scale in northern Serbia and southern Slovakia, situated at opposite edges of the Pannonian Plain. In the affected locations where the disease was assessed, symptomatic sugar beets were analysed for phytoplasma infection. Additionally, multilocus sequence analyses of ‘Ca. P. solani’ strains on epidemiologically informative marker genes (tuf, stamp and vmp1) were performed. Symptomatic sugar beets from other countries of the Pannonian Plain (Croatia, Hungary and Austria), one sample from Germany, and red beets from Serbia were included in the analyses. ‘Ca. P. solani’ was detected in sugar beet in all assessed countries, as well as in red beet. Molecular analyses revealed the high genetic variability of ‘Ca. P. solani’ with the presence of all four tuf-types (a, b1, b2 and d), 14 stamp genotypes (seven new) and five vmp1 profiles (one new). The most common multilocus genotype in Serbia, Slovakia, Croatia, and Hungary was dSTOLg (tuf-d/STOL/V2-TA). It was dominant on sites with epidemic RTD outbreaks in the Pannonian Plain and in several sugar beet fields with non-epidemic RTD occurrence suggesting the prevalence of a particular epidemiological pathway during the epidemic’s phases.

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Section: Introductionmentioning

confidence: 99%

Multilocus Genotyping of ‘Candidatus Phytoplasma solani’ Associated with Rubbery Taproot Disease of Sugar Beet in the Pannonian Plain

et al. 2021

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“…The RFLP analysis conducted on stamp gene amplicons revealed the presence of five profiles (Table 2). A phylogenetic tree was constructed using representative nucleotide sequences of the stamp gene obtained in this study and 70 stamp sequences retrieved from previous studies [19,27,44,46]. The phylogenetic analysis showed the presence of 11 stamp variants (St1-3, St2-11, St3-2, St4-3, St5-8, St8-4, St9-1, St10-2, St11-2, St18-2, and St19-6) determined by comparison with the available stamp gene dataset [44,46].…”

Section: Resultsmentioning

confidence: 99%

“…A phylogenetic tree was constructed using representative nucleotide sequences of the stamp gene obtained in this study and 70 stamp sequences retrieved from previous studies [19,27,44,46]. The phylogenetic analysis showed the presence of 11 stamp variants (St1-3, St2-11, St3-2, St4-3, St5-8, St8-4, St9-1, St10-2, St11-2, St18-2, and St19-6) determined by comparison with the available stamp gene dataset [44,46]. Two variants identified in tomato from Portugal and grapevine from Spain were found for the first time in the present study and were deposited at NCBI GenBank with the accession numbers MW759855 (tomato P3) and MW759856 (grapevine 3S) (Table 3).…”

Section: Resultsmentioning

confidence: 99%

“…The vmp1 gene sequences were trimmed to approximately 1,300 nt and the stamp gene sequences to approximately 500 nt, and phylogenetic analyses were carried out with MEGA X [42] using the neighbor-joining method [43], with 1000 bootstrap replicates to estimate the solidity of the analysis. Phylogenetic trees were constructed based on nucleotide sequences of vmp1 and stamp genes produced in this work, strain's sequences from previous studies [17,26,44,45] and retrieved from the NCBI GenBank (Table 1). Stamp gene nucleotide sequences were analyzed by sequence identity matrix to calculate their genetic diversity and aligned with 70 sequences of previously defined stamp sequence variants [26,44,45].…”

Section: Sequencing and Phylogenetic Analysesmentioning

confidence: 99%

“…Phylogenetic trees were constructed based on nucleotide sequences of vmp1 and stamp genes produced in this work, strain's sequences from previous studies [17,26,44,45] and retrieved from the NCBI GenBank (Table 1). Stamp gene nucleotide sequences were analyzed by sequence identity matrix to calculate their genetic diversity and aligned with 70 sequences of previously defined stamp sequence variants [26,44,45]. A nucleotide sequence identity of 100% was employed for the sequence variant attribution.…”

Section: Sequencing and Phylogenetic Analysesmentioning

confidence: 99%

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Molecular Variability and Host Distribution of ‘Candidatus Phytoplasma solani’ Strains from Different Geographic Origins

et al. 2021

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The knowledge of phytoplasma genetic variability is a tool to study their epidemiology and to implement an effective monitoring and management of their associated diseases. ‘Candidatus Phytoplasma solani’ is associated with “bois noir” disease in grapevines, and yellowing and decline symptoms in many plant species, causing serious damages during the epidemic outbreaks. The epidemiology of the diseases associated with this phytoplasma is complex and related to numerous factors, such as interactions of the host plant and insect vectors and spreading through infected plant propagation material. The genetic variability of ‘Ca. P. solani’ strains in different host species and in different geographic areas during the last two decades was studied by RFLP analyses coupled with sequencing on vmp1, stamp, and tuf genes. A total of 119 strains were examined, 25 molecular variants were identified, and the variability of the studied genes was linked to both geographic distribution and year of infection. The crucial question in ‘Ca. P. solani’ epidemiology is to trace back the epidemic cycle of the infections. This study presents some relevant features about differential strain distribution useful for disease monitoring and forecasting, illustrating and comparing the phytoplasma molecular variants identified in various regions, host species, and time periods.

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Identification of phytoplasmas associated with grapevine ‘bois noir’ and flavescence dorée in inter-row groundcover vegetation used for green manure in Franciacorta vineyards

Moussa

Guerrieri

Torcoli³

et al. 2023

J Plant Pathol

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Abstract‘Bois noir’ (BN) and flavescence dorèe (FD), the two main diseases of the grapevine yellows complex associated with genetically distinct phytoplasmas, have a complex epidemiology including multiple insect vectors and reservoir plants. This study investigated the presence of BN and FD phytoplasmas in nine groundcover plant species commonly utilized for inter-row vineyard green manure in Franciacorta (North Italy). The activities conducted in 2020 included monitoring and sampling groundcover plant species and symptomatic grapevines in September, and phytoplasma identification and typing by amplification and sequence analyses of stamp and map genes. Molecular analyses identified BN phytoplasma (strains carrying the stamp gene sequence variants St5, St19, St30) and FD phytoplasma (strains carrying the map gene sequence variant M54) in 72% and 28% of symptomatic grapevines, respectively. BN phytoplasma strains St5 and St30 were found also in Eruca sativa, Vicia sativa, and Polygonum fagopyrum. FD phytoplasma strain M54 was found also in Vicia faba, Trifolium incarnatum, and Polygonum fagopyrum. These results reinforced the evidence of the increasing range of BN and FD phytoplasma alternative plant hosts and suggested a criterium for the selection of the groundcover plant species utilized for green manure, excluding the ones putatively involved in BN and FD diffusion.

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Molecular and spatial analyses reveal new insights on Bois noir epidemiology in Franciacorta vineyards

Cited by 14 publications

References 46 publications

Multilocus Genotyping of ‘Candidatus Phytoplasma solani’ Associated with Rubbery Taproot Disease of Sugar Beet in the Pannonian Plain

Multilocus Genotyping of ‘Candidatus Phytoplasma solani’ Associated with Rubbery Taproot Disease of Sugar Beet in the Pannonian Plain

Molecular Variability and Host Distribution of ‘Candidatus Phytoplasma solani’ Strains from Different Geographic Origins

Identification of phytoplasmas associated with grapevine ‘bois noir’ and flavescence dorée in inter-row groundcover vegetation used for green manure in Franciacorta vineyards

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