Molecular Approaches for Studying Root Herbivores

Benefer, Carly M.; Blackshaw, R. P.

doi:10.1016/b978-0-12-417165-7.00005-2

Cited by 4 publications

(3 citation statements)

References 102 publications

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“…In Collembola, it has been shown that occurrence-habitat preference relationships were better explained at the family level, where species in the same family with similar habitat preferences congregated, but this was less clear at the genus and species levels, possibly due to competitive exclusion at low taxonomic levels, and convergent evolution/co-adaptation at high taxonomic levels (Ponge and Salmon, 2013). To some extent larval dispersal ability and adult oviposition preferences may be involved in this, but for many soil insect taxa this is largely unknown (Benefer and Blackshaw, 2013). This suggests that there may not be a straightforward relationship between co-occurrence and taxonomic level.…”

Section: Species Distribution Across Spatial Scales and Yearsmentioning

confidence: 99%

“…Further investigation of the impact of this taxonomic component on soil insect distribution (previous studies have mainly been carried out at the mesofaunal level; e.g., Ponge and Salmon, 2013) would aid our understanding of how biological components, such as phylogenetic relationship and species traits, interact with scale and spatial effects. DNA based techniques, such as metabarcoding, yet to be applied to soil insect ecological studies, may be of use here (Benefer and Blackshaw, 2013), particularly across multiple sites and over time where the large sample size precludes ecological studies using traditional morphological identification and soil processing techniques.…”

Section: Species Distribution Across Spatial Scales and Yearsmentioning

confidence: 99%

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The Distribution of Soil Insects across Three Spatial Scales in Agricultural Grassland

Benefer

D'Ahmed

Blackshaw³

et al. 2016

Front. Ecol. Evol.

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The effects of specific environmental factors on abundance and distribution of some individual soil insect taxa is known, but how scale influences spatial distribution is less well evaluated, particularly at the community level. However, given that many soil insects are pests or beneficial natural enemies, and that collectively they play a role in soil processes, this information is of potential value for predictive modeling and in furthering our understanding of soil ecology and management. The objectives of this study were to characterize the spatial distribution, relative population sizes, effect of sampling scale and taxa co-occurrence on a range of soil insects at the family level over 2 years. Soil cores were taken from agricultural grassland soils across three different sampling scales (farm, field, and core) using a systematic sampling approach. Spatial distribution was assessed using the variance-to-mean (VMR) ratio and taxa distribution plots and the contribution of scale, spatial (geographical location), and biotic (presence-absence of other species) factors determined using deviance partitioning. Tipulid larvae (leatherjackets) were the most abundant taxa in both years, but the composition of other Dipteran and Coleopteran taxa varied between years. The VMRs revealed differences in spatial distribution between taxa across scales and years, showing a range of underlying distributional patterns. Scale was the most important factor influencing species distributions, but a large proportion of deviance remained unexplained and there was much variation between taxa, suggesting biological and scale-specific factors are driving distributions, in agreement with a previous study.

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Section: Species Distribution Across Spatial Scales and Yearsmentioning

confidence: 99%

Section: Species Distribution Across Spatial Scales and Yearsmentioning

confidence: 99%

The Distribution of Soil Insects across Three Spatial Scales in Agricultural Grassland

Benefer

D'Ahmed

Blackshaw³

et al. 2016

Front. Ecol. Evol.

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“…DNA barcoding (Hebert et al ., ), sequencing a section of the cytochrome c oxidase subunit I (COI) mitochondrial protein coding gene, has become a standard for identification of closely‐related taxa across a range of taxonomic groups and, as such, has been used to distinguish morphologically cryptic species in many taxa. However, to date, the application of molecular genetic techniques to soil insects has lagged behind their aboveground counterparts (Benefer & Blackshaw, ). Where they have been used, at the COI region and other diagnostic regions of DNA, it has often proven to be invaluable for clarifying previously unclear aspects of the biology and ecology of a range of species (e.g.…”

Section: Introductionmentioning

confidence: 99%

Molecular identification and distribution of leatherjackets (Diptera: Tipulidae) in U.K. agricultural grassland

Benefer

D'Ahmed

Murray

et al. 2017

Agri and Forest Entomology

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1 DNA barcoding is useful for the identification of morphologically cryptic invertebrates. An important application is for pest species, for which it is critical to determine the distribution, biology and ecology of damaging life stages in order to target management effectively. 2 Tipula paludosa (Meigen) and Tipula oleracea (Linnaeus) leatherjackets, the larvae of crane flies (Diptera: Tipulidae), are pests of agricultural grassland in Europe and the U.S.A. and are difficult to distinguish morphologically. 3 We used cytochrome c oxidase subunit I (COI) barcoding to identify leatherjackets from 19 permanent grassland fields over 2 sampling seasons on the Rothamsted Research North Wyke Farm Platform, south west UK, to assess species-level distribution and genetic diversity. 4 Most larvae (94%) were found to be T. paludosa, comprising 18 haplotypes that were spread across the sampling site in a panmictic population. However, T. oleracea were found in low abundance (3% of larval samples) and only in the second year of sampling. Other morphologically similar Dipteran larvae (3%) were also found. 5 This dominance of one species suggests that there may be underlying differences in species biology, such as choice of oviposition site and dispersal ability, which is in agreement with other studies and has implications for monitoring and management.

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DNA barcoding identification and genetic diversity of bamboo shoot wireworms (Coleoptera: Elateridae) in South China

Zhang¹,

Liu²,

Shu³

et al. 2019

Journal of Asia-Pacific Entomology

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Molecular Approaches for Studying Root Herbivores

Cited by 4 publications

References 102 publications

The Distribution of Soil Insects across Three Spatial Scales in Agricultural Grassland

The Distribution of Soil Insects across Three Spatial Scales in Agricultural Grassland

Molecular identification and distribution of leatherjackets (Diptera: Tipulidae) in U.K. agricultural grassland

DNA barcoding identification and genetic diversity of bamboo shoot wireworms (Coleoptera: Elateridae) in South China

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