Molecular aspects of affinity chromatography

Abstract

Affinity chromatography is based on the well‐known specific interaction between biomacromolecules and between biomacromolecules and low‐molecular partners. To separate the one component (bioaffine component), the complementary bioaffinant has to be attached chemically to a solid‐phase support. This interaction, however, can be disturbed considerably by the linkage of one partner to the insoluble matrix. Some molecular aspects of this interaction discussed in a summarized presentation of our investigations are: (1) conformational state of immobilized biomacromolecules; (2) nonspecific interaction between matrix and bioaffine component; (3) steric factors; and (4) interaction between spacer and/or carrier and bioaffine components.

Spectroscopic, thermal, and conductometric investigation of some (arylazo)quinolin-8-ol and their complexes with the divalent ions of Mn, Ni, Cu, and Zn

“…Hydroxyquinolines have been used at different times in organic, inorganic, and analytical [4] Among different types of dyes used for the purpose, azo dyes have a distinct advantage over others [5]. Furthermore, quinolin-8-ol derivatives may be used as chelate forming agents in chelate a nity chromatography [6]. These dyes are used as probes in protein chemistry to study the binding behavior of proteins [7], [8].…”