Molecular aspects of skin ageing

Naylor, Elizabeth C.; Watson, Rachel; Sherratt, Michael J.

doi:10.1016/j.maturitas.2011.04.011

Cited by 482 publications

(462 citation statements)

References 94 publications

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“…Current knowledge suggests that thus is the key pathogenic mechanism involved in photoaging. In various studies [21][22][23][24], it has been shown that exposure of skin to UVR induces epidermal expression of three main metalloproteinases -namely, collagenase, gelatinase, and stromelysin. These proteins are translocated through the basement membrane zone to the dermis and degrade collagen [16].…”

Section: The Proportion Of the Immunoreactive Cells In Skin Biopsiesmentioning

confidence: 99%

One week’s holiday sun exposure induces expression of photoaging biomarkers

Lesiak

Rogowski-Tylman

Danilewicz

et al. 2015

Folia Histochem Cytobiol.

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Introduction. Skin aging is accompanied by the upregulation of the expression of various matrix metalloproteinases (MMPs). It was shown that exposure to ultraviolet radiation (UVR) may induce skin expression of MMPs and dysregulation of the transforming growth factor beta (TGF-b)/Smad pathway. The aim of our study was to compare the effects of short holiday UVR exposure and lifetime UVR exposure, on the expression of MMP-8, TGF-b1, and Smad2 in human skin biopsies. Material and methods. Skin biopsies were taken from the outer upper arm of 15 elderly people with significant photoaging (mean age 64.1 years) (Group 1) and from 15 healthy young adult volunteers (mean age 24.1 y) who participated in a six-day sun holiday. Biopsies were taken twice: 24 hours before leaving for holiday (Group 2a) and 24 hours after returning (Group 2b). The expression of TGF-b1, Smad2, and MMP-8 was examined by immunochemistry and measured semiquantitatively by two independent pathologists. Results. The mean expression of TGF-b1 in dermal fibroblasts and keratinocytes in Group 1 and Group 2b was significantly lower than in Group 2a (0.54% ± 0.44% and 0.48% ± 0.51% vs. 1.48% ± 0.72%, respectively). The percentage of Smad2 (+) cells in Group 1 and Group 2b was lower than in Group 2a (2.13% ± 1.39% and 1.81% ± 1.16% vs. 4.13% ± 1.58%, respectively). The MMP-8 expression in Group 2b was 1.36% ± 0.68% and was significantly higher than in Group 1 (0.34% ± 0.42%) and Group 2a in which the protein was not detected (p < 0.001). Conclusions. We conclude that the decrease in the expression of TGF-b1 and Smad2 is a persistent biomarker of skin photoaging, while the increased expression of MMP-8 in keratinocytes can be regarded as a marker of acute sun exposure.

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Section: The Proportion Of the Immunoreactive Cells In Skin Biopsiesmentioning

confidence: 99%

One week’s holiday sun exposure induces expression of photoaging biomarkers

Lesiak

Rogowski-Tylman

Danilewicz

et al. 2015

Folia Histochem Cytobiol.

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“…Skin aging occurs through two distinct processes: intrinsic and extrinsic aging [12,13]. The former is a naturally occurring process, while the latter occurs due to environmental factors (e.g., exposure to ultraviolet light).These aging processes appear in the facial skin.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of aging, diabetes mellitus, and skin wounds by scanning acoustic microscopy with protease digestion

Miura

Yamashita

2018

Pathobiology of Aging & Age-related Diseases

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Scanning acoustic microscopy (SAM) can assess tissue stiffness by calculating the speed of sound (SOS) through tissues. SOS increases as tissue stiffness increases. Sensitivity to protease digestion depends on protein type, concentration, and modification. We analyzed the SOS images of formalin-fixed paraffin-embedded skin sections from elderly, young, diabetic, and nondiabetic subjects, as well as chronic and acute wounds. SAM provided high-resolution histology similar to LM and revealed characteristic SOS alteration following pepsin treatment. SOS values of dermis samples from elderly subjects (especially females) were lower than those of younger adults, which was indicative of age-related dermal softening and loosening. SOS values of elderly females were lower than those of younger females and elderly males. Dermal SOS showed a positive correlation with epidermal thickness. SOS values of epidermis of elderly subjects were higher than those of younger adults and showed a rapid decline 0.5h after protease digestion. Reticular dermis of diabetic patients exhibited greater pepsin resistance than that of nondiabetic patients. Chronic wounds exhibited greater SOS values and pepsin resistance than acute wounds. SOS variation with aging, diabetes mellitus, and wound fibrosis reflected histological and mechanical changes associated with senescence and disease duration. Epidermal thickness reflects age-related changes in dermal stiffness.

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“…In aged skin, the number of keratinocyte and fibroblast decreases and results in skin thinning and reduction of connective tissue. Skin connective tissue is primarily comprised of fibrillar collagen bundles and elastic fibers that fill extracellular spaces [27]. Collagen and elastic fibers are responsible for the structural and elastic qualities of skin and gradually decrease with age.…”

Section: Effect Of Daidzein On the Expression Of Ecm Componentsmentioning

confidence: 99%

The Phytoestrogenic Effect of Daidzein in Human Dermal Fibroblasts

Kim¹,

Hong²,

Lee³

2014

Journal of the Society of Cosmetic Scientists of Korea

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Abstract:Estrogen deficiency results in a reduction of skin quality and function in postmenopausal women. Over the past decade, many studies have supported that estrogen provides anti-aging effects as a result of the ability of estrogen to prevent skin collagen decline, restore skin elasticity, and increase skin hydration in postmenopausal women skin. Due to their structural similarity with estrogen, isoflavones have been called phytoestrogens. Photoprotective effects of isoflavones are well established while their estrogenic-like activities are not fully understood in human skin. In this study, we investigated whether daidzein, an effective isoflavone, has phytoestrogenic activity and induces transcriptional change of extracellular matrix components in dermal fibroblasts. We examined the luciferase activity of daidzein and β -estradiol using transiently transfected NIH3T3-ERE cells. The estrogenic receptor-dependent transcriptional activity was increased in a dose-dependent manner when treated with daidzein, with a maximum of 2.5-fold induction at 10 µg/mL of daidzein compared with non-treated control. In addition, daidzein significantly in creased the expressions of collagen 1) † 주 저자 (e-mail: misunkim@lgcare.com) 2014 type I, collagen type IV, elastin, and fibrillin-1 in human dermal fibroblasts. By comparing with the effects of β -estradiol through out all the experiments, we confirmed that daidzein had estrogenic activity and function in fibroblasts. These results suggest that daidzein-based application, having both photoprotective and phytoestrogenic effects, may be a powerful approach for skin anti-aging of postmenopausal women.

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Molecular aspects of skin ageing

Cited by 482 publications

References 94 publications

One week’s holiday sun exposure induces expression of photoaging biomarkers

One week’s holiday sun exposure induces expression of photoaging biomarkers

Evaluation of aging, diabetes mellitus, and skin wounds by scanning acoustic microscopy with protease digestion

The Phytoestrogenic Effect of Daidzein in Human Dermal Fibroblasts

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