Molecular assessment of Bartonella in Gerbillus nanus from Saudi Arabia reveals high levels of prevalence, diversity and co-infection

Kleynhans, Dewald J.; Sarli, Joshua; Hatyoka, Luiza M.; Alagaili, Abdulaziz N.; Bennett, Nigel C.; Mohammed, Osama B.; Bastos, Armanda D.S.

doi:10.1016/j.meegid.2018.07.036

Cited by 7 publications

(3 citation statements)

References 53 publications

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“…Similarly, the 33.8% co-infection level in A. ineptus is nearly two-fold higher than the 18.2% recorded for M. namaquensis (Brettschneider et al, 2012). Although this co-infection rate is higher than the 22% maximum generally reported in studies employing similar experimental procedures to ours (Birtles et al, 2001;Brettschneider et al, 2012;Gundi et al, 2010;Inoue et al, 2009;Kosoy et al, 2004c;Telfer et al, 2007), it is on par with that reported for Baluchistan gerbils (Gerbillus nanus) from Saudi Arabia (Kleynhans et al, 2018). In common with A. ineptus, the latter species also displayed high levels of infection and lineage diversity.…”

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confidence: 43%

“…A multi-locus sequence analysis (MLSA) approach, was used to evaluate Bartonella genome presence in DNA extracts from heart samples of 75 A. ineptus (40 females, 35 males) sampled as previously described (Muteka et al 2006). Briefly, the citrate synthase (gltA), NADH dehydrogenase gamma subunit (nuoG), riboflavin synthase (ribC), and RNA polymerase subunit B (rpoB) gene regions were amplified with genus-specific primers, as previously described (Hatyoka et al 2018). Amplicons of the expected size were purified and submitted to the core Sanger sequencing facility of the University of Pretoria.…”

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confidence: 99%

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Bartonella diversity and zoonotic potential in indigenous Tete Veld rats (Aethomys ineptus) from South Africa

Hatyoka

Brettschneider

Bennett

et al. 2019

Infection, Genetics and Evolution

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confidence: 43%

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confidence: 99%

Bartonella diversity and zoonotic potential in indigenous Tete Veld rats (Aethomys ineptus) from South Africa

Hatyoka

Brettschneider

Bennett

et al. 2019

Infection, Genetics and Evolution

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“…Several different methods have been previously used to identify the species present in co-infected Bartonella samples. Some studies have compared the chromatographs of ambiguous sequences to those of unambiguous sequences, whereas others have used culturing techniques or cloning [14,30,31]. These methods are tedious, time-consuming, and may not resolve all mixed infections.…”

Section: Discussionmentioning

confidence: 99%

Adaptation of gltA and ssrA assays for diversity profiling by Illumina sequencing to identify Bartonella henselae, B. clarridgeiae and B. koehlerae

Power

Calvani

Nachum‐Biala

et al. 2021

Journal of Medical Microbiology

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Introduction. Bartonellosis is an emerging zoonotic disease caused by bacteria of the genus Bartonella . Mixed Bartonella infections are a well-documented phenomenon in mammals and their ectoparasites. The accurate identification of Bartonella species in single and mixed infections is valuable, as different Bartonella species have varying impacts on infected hosts. Gap Statement. Current diagnostic methods are inadequate at identifying the Bartonella species present in mixed infections. Aim. The aim of this study was to adopt a Next Generation Sequencing (NGS) approach using Illumina sequencing technology to identify Bartonella species and demonstrate that this approach can resolve mixed Bartonella infections. Methodology. We used Illumina PCR amplicon NGS to target the ssrA and gltA genes of Bartonella in fleas collected from cats, dogs and a hedgehog in Israel. We included artificially mixed Bartonella samples to demonstrate the ability for NGS to resolve mixed infections and we compared NGS to traditional Sanger sequencing. Results. In total, we identified 74 Ctenocephalides felis, two Ctenocephalides canis, two Pulex irritans and three Archaeopsylla e. erinacei fleas. Real-time PCR of a subset of 48 fleas revealed that twelve were positive for Bartonella , all of which were cat fleas. Sanger sequencing of the ssrA and gltA genes confirmed the presence of Bartonella henselae , Bartonella clarridgeiae and Bartonella koehlerae . Illumina NGS of ssrA and gltA amplicons further confirmed the Bartonella species identity in all 12 flea samples and unambiguously resolved the artificially mixed Bartonella samples. Conclusion. The adaptation and multiplexing of existing PCR assays for diversity profiling via NGS is a feasible approach that is superior to traditional Sanger sequencing for Bartonella speciation and resolving mixed Bartonella infections. The adaptation of other PCR primers for Illumina NGS will be useful in future studies where mixed bacterial infections may be present.

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Molecular epidemiology of certain vector-borne bacterial microorganisms in domestic animals and their ectoparasites in Egypt

Abdullah

Elbayoumy

Allam

et al. 2021

Trop Anim Health Prod

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Molecular assessment of Bartonella in Gerbillus nanus from Saudi Arabia reveals high levels of prevalence, diversity and co-infection

Cited by 7 publications

References 53 publications

Bartonella diversity and zoonotic potential in indigenous Tete Veld rats (Aethomys ineptus) from South Africa

Bartonella diversity and zoonotic potential in indigenous Tete Veld rats (Aethomys ineptus) from South Africa

Adaptation of gltA and ssrA assays for diversity profiling by Illumina sequencing to identify Bartonella henselae, B. clarridgeiae and B. koehlerae

Molecular epidemiology of certain vector-borne bacterial microorganisms in domestic animals and their ectoparasites in Egypt

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