Molecular authentication of geo-authentic Scrophularia ningpoensis

Chen, Chuan; Duan, Li-na; Zhou, Xiaolong; Chen, Bing-long; Fu, Chengxin

doi:10.1631/jzus.b1000179

Cited by 11 publications

(7 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results revealed that this SCAR marker might be used in identification and authentication of C. wightii . Many reports are available in which SCAR markers have been used for authentication of medicinal plant species like Panax ginseng [ 65 ], bent-grass [ 66 ], Bamboo [ 67 ], Piper longum [ 68 ], Artemisia princeps and A. argyi [ 69 ], Phyllanthus emblica [ 70 ], strawberry [ 71 ] Jatropha curcas [ 72 , 73 ], Ganoderma lucidum [ 74 ], Pueraria tuberosa [ 75 ], Dendrobium candidum [ 76 ], Sinapis arvensis [ 77 ], Cornus officinalis [ 78 ], and Scrophularia ningpoensis [ 79 ]. Three SCAR markers of Phyllanthus species were developed from three specific RAPD sequences that can identify and differentiate the morphologically similar Phyllanthus species [ 80 ].…”

Section: Discussionmentioning

confidence: 99%

SCAR Marker for Identification and Discrimination of Commiphora wightii and C. myrrha

Sairkar

Sharma

Shukla

2016

Molecular Biology International

View full text Add to dashboard Cite

Commercially important Commiphora species are drought-tolerant plants and they are leafless for most of the year. Therefore, it is necessary to develop some molecular marker for the identification. Intended for that, in the present study, species-specific, sequence-characterized amplified regions (SCAR) markers were developed for proficient and precise identification of closely related species Commiphora wightii and C. myrrha, which may ensure the quality, safety, and efficacy of medicines made from these plants through adulterous mixing of these plants. Two species-specific RAPD amplicons were selected, gel-purified, cloned, and sequenced after screening of 20 RAPD primers. The sequence of 979 and 590 nucleotides (Genebank accession numbers K90051 and K90052) was used for development of 4 SCAR markers, namely, Sc1P, Sc1Pm, Sc2P, and Sc2Pm. Out of them, the Sc1Pm was specific for C. wightii, while Sc2P discriminated both the Commiphora species. These markers are first reported and will be useful for rapid identification of closely related Commiphora wightii and C. myrrha species.

show abstract

Section: Discussionmentioning

confidence: 99%

SCAR Marker for Identification and Discrimination of Commiphora wightii and C. myrrha

Sairkar

Sharma

Shukla

2016

Molecular Biology International

View full text Add to dashboard Cite

show abstract

“…(SNH, known as Xuanshen ) is a perennial herb native to Southeastern China. SNH cultivated in Zhejiang is a geo‐authentic crude drug among the most famous “Zhebawei” and “Panwuwei ” herbal medicines [6] . Scrophulariae Radix (SR), the dried root of SNH, has been used as a traditional Chinese medicine for centuries to treat various diseases including sore throat, abscesses, carbuncles and constipation [7] .…”

Section: Introductionmentioning

confidence: 99%

“…SNH cultivated in Zhejiang is a geo-authentic crude drug among the most famous "Zhebawei" and "Panwuwei" herbal medicines. [6] Scrophulariae Radix (SR), the dried root of SNH, has been used as a traditional Chinese medicine for centuries to treat various diseases including sore throat, abscesses, carbuncles and constipation. [7] SR is also used as the additive in health care products and functional foods.…”

Section: Introductionmentioning

confidence: 99%

Molecular Insights into the Macrophage Immunomodulatory Effects of Scrophulariae Radix Polysaccharides

Zhao,

Zheng,

Wang

et al. 2023

Chemistry & Biodiversity

View full text Add to dashboard Cite

Scrophulariae Radix (SR) has been widely used in Chinese herbal compound prescriptions, health care products and functional foods. The present study aimed to investigate the immunomodulatory activity of polysaccharides from SR (SRPs) in macrophages and explore the potential mechanisms. The results showed that four SRPs fractions (SRPs40, SRPs60, SRPs80 and SRPs100) had similar absorption peaks and monosaccharide compositions, but the intensities of absorption peaks and monosaccharide contents were distinguished. All SRPs fractions significantly enhanced the pinocytic activity, promoted the production of NO and TNF‐α, increased the mRNA expressions of inflammatory factors (IL‐1β, IL‐6, TNF‐α and PTGS2) and TLR2, and elevated the phosphorylation levels of p38, ERK, JNK, p65 and IκB. Moreover, the production of NO and TNF‐α stimulated by SRPs was dramatically suppressed by anti‐TLR2 antibody. These results indicated that SRPs activated macrophages through MAPK and NF‐κB signaling pathways via recognition of TLR2.

show abstract

“…It is known that the bioactive constituency, medicinal quality and efficacy of Chinese herbs are determined by their geo-authentic features (Chen et al 2011;Woo et al 1999;Liang et al 2013). Moreover, compared with other herbs, high-quality of herbs are better in clinical effects.…”

Section: Introductionmentioning

confidence: 99%

Determination of organic acids for quality evaluation in Coptis herbs by ion chromatography

Zhou

Wang

et al. 2018

3 Biotech

View full text Add to dashboard Cite

herbs are important herbal medicinal materials. The bioactive composition, the quality and medicinal efficacy of these herbs, are determined significantly by their geo-authentic features. Among the effective components of these herbs are seven organic acids (quinic, acetic, formic, tartaric, malic, succinic, and oxalic acids). However, no quantitative data of these seven acids in these herbs are available. Therefore, we developed a method for simultaneous separation and determination of the seven organic acids in herbs using gradient ion chromatography (mobile phase and gradient were shown in Table 1). The seven acids were separated and determined in no more than 35 min. We found that the organic acid levels in was obviously higher than in and, in particular, the content of quinic acid in was about eight times than that in and . Furthermore, we analyzed the relationships between the contents of organic acids and clinical effects, and found that organic acids (content of total acids or content of quinic acid) could act as an reference ingredient for quality evaluation in herbs. Our studies would lay the foundation for effective quality evaluation of these herbs.

show abstract

Molecular authentication of geo-authentic Scrophularia ningpoensis

Cited by 11 publications

References 30 publications

SCAR Marker for Identification and Discrimination of Commiphora wightii and C. myrrha

SCAR Marker for Identification and Discrimination of Commiphora wightii and C. myrrha

Molecular Insights into the Macrophage Immunomodulatory Effects of Scrophulariae Radix Polysaccharides

Determination of organic acids for quality evaluation in Coptis herbs by ion chromatography

Contact Info

Product

Resources

About