Molecular bases for the constitutive photomorphogenic phenotypes in <i>Arabidopsis</i>

Pham, Vinh Ngoc; Xu, Xiaosa; Huq, Enamul

doi:10.1242/dev.169870

Cited by 55 publications

(31 citation statements)

References 67 publications

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“…Parallel to and partially dependent on COP1/SPA, the PIFs also repress light signalling. Light‐activated phytochromes bind PIFs and repress their activity by promoting their phosphorylation and targeting for degradation as well as by inhibiting their association with target promoters (Bauer et al ., 2004; Shen et al ., 2005; Al‐Sady et al ., 2006; Shen et al ., 2008; Park et al ., 2012; Lee and Choi, 2017; Pham et al ., 2018; Paik and Huq, 2019).…”

Section: Introductionmentioning

confidence: 99%

COLD REGULATED 27 and 28 are targets of CONSTITUTIVELY PHOTOMORPHOGENIC 1 and negatively affect phytochrome B signalling

et al. 2020

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Phytochromes are red/far-red light receptors in plants involved in the regulation of growth and development. Phytochromes can sense the light environment and contribute to measuring day length; thereby, they allow plants to respond and adapt to changes in the ambient environment. Two well-characterized signalling pathways act downstream of phytochromes and link light perception to the regulation of gene expression. The CONSTITUTIVELY PHOTOMORPHOGENIC 1/SUPPRESSOR OF PHYA-105 (COP1/SPA) E3 ubiquitin ligase complex and the PHYTOCHROME INTERACTING FACTORs (PIFs) are key components of these pathways and repress light responses in the dark. In light-grown seedlings, phytochromes inhibit COP1/SPA and PIF activity and thereby promote light signalling. In a yeast-two-hybrid screen for proteins binding to light-activated phytochromes, we identified COLD-REGULATED GENE 27 (COR27). COR27 and its homologue COR28 bind to phyA and phyB, the two primary phytochromes in seed plants. COR27 and COR28 have been described previously with regard to a function in the regulation of freezing tolerance, flowering and the circadian clock. Here, we show that COR27 and COR28 repress early seedling development in blue, farred and in particular red light. COR27 and COR28 contain a conserved Val-Pro (VP)-peptide motif, which mediates binding to the COP1/SPA complex. COR27 and COR28 are targeted for degradation by COP1/SPA and mutant versions with a VP to AA amino acid substitution in the VP-peptide motif are stabilized. Overall, our data suggest that COR27 and COR28 accumulate in light but act as negative regulators of light signalling during early seedling development, thereby preventing an exaggerated response to light.

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Section: Introductionmentioning

confidence: 99%

COLD REGULATED 27 and 28 are targets of CONSTITUTIVELY PHOTOMORPHOGENIC 1 and negatively affect phytochrome B signalling

et al. 2020

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“…On the contrary, COP1 is regarded as a central negative factor on plant photomorphogenesis [22,23]. It has been reported that COP1-SPAs complex not only target positive regulators (HY5, HFR1, etc) for ubiquitination and degradation, but also promote the accumulation of negative factors such as PIFs [17,24,25]. We found four COP1 transcripts, among which only one transcript had relative high expression.…”

Section: Key Regulators In Light Signaling Pathwaymentioning

confidence: 71%

“…Although the effects of light quality on photomorphogenesis and the underlying molecular mechanism has been well studied in Arabidopsis [16][17][18], it is poorly understood in other plant species. We tested the effects of light quality on the hypocotyl elongation in eight eudicot species.…”

Section: Resultsmentioning

confidence: 99%

Transcriptional Regulation of Photomorphogenesis in Brassica Napus Under Different Light Quality

Zhang¹,

Liu²,

Huang³

et al. 2020

Preprint

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Background: Plants undergo skotomorphogenesis or photomorphogenesis upon the absence or presence of light. Although the light signaling pathway has been well documented in Arabidopsis, relatively little is known in other plant species including Brassica napus.Results: In this study, we examined the response of hypocotyl elongation to different light quality (white, blue, red and far-red light) in B. napus. The result showed each of tested light quality promoted photomorphogenesis, with less effect of red light than blue/far-red light. Subsequently, RNA-sequencing was performed to obtain the transcriptomic profile in B. napus in response to dark and different light quality. A total of 9748 genes were differentially expressed among treatments. Correlation analysis and hierarchical cluster analysis of differentially expressed genes (DEGs) indicated the transcriptomic profile in red light exhibited an analogous pattern with that in dark to some extent, while blue/far-red light induced transcriptional profiles showed distinct patterns. Moreover, we found the differential expressions of PHYB, COP1 and BBXs homologs between two subgenomes, which may affect their interactions with up/downstream pathway. Finally, we constructed a transcriptional network including light signaling, phytohormone and cell elongation/modification, which explained the observed hypocotyl phenotype under different light quality.Conclusions: We partially explained why red light has reduced effect on photomorphogenesis in B. napus by RNA-seq and displayed gene expression of the key regulators in light signaling pathway and their contribution to photomorphogenesis under different light quality in B. napus. The result could improve new sight into understanding on transcriptional regulation of photomorphogenesis of B. napus.

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“…; Pham et al . ), and sunflower is a model organism used to study global warming (Badoin et al . ), the pioneering S. alba work of Mohr and collaborators laid the foundations for these more recent developments in plant sciences.…”

Section: Discussionmentioning

confidence: 99%

Photomorphogenesis of the root system in developing sunflower seedlings: a role for sucrose

Kutschera

Briggs

2019

Plant Biol J

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The domestic sunflower (Helianthus annuus L. cv. 'Giganteus') has been used since the 19th century as a model plant for the study of seedling development in darkness and white light (WL) (scoto-versus photomorphogenesis). However, most pertinent studies have focused on the developmental patterns of the hypocotyl and cotyledons, whereas the root system has been largely ignored.• In this study, we analysed entire sunflower seedlings (root and shoot) and quantified organ development in the above-and belowground parts of the organism under natural (non-sterile) conditions.• We document that seedlings, raised in moist vermiculite, are covered with methylobacteria, microbes that are known to promote root development in Arabidopsis. Quantitative data revealed that during photomorphogenesis in WL, the root system expands by 90%, whereas stem elongation is inhibited, and hook opening/cotyledon expansion occurs. Root morphogenesis may be mediated via imported sucrose provided by the green, photosynthetically active cotyledons. This hypothesis is supported by the documented effect of sucrose on the induction of lateral root initials in sunflower cuttings. Under these experimental conditions, phytohormones (auxin, cytokinin, brassinolide) exerted little effect on root and cotyledon expansion, and no hormone-induced initiation of lateral roots was observed.• It is concluded that sucrose not only acts as an energy source to fuel cell metabolism but is also a shoot-derived signalling molecule that triggers root morphogenesis.

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Molecular bases for the constitutive photomorphogenic phenotypes in Arabidopsis

Cited by 55 publications

References 67 publications

COLD REGULATED 27 and 28 are targets of CONSTITUTIVELY PHOTOMORPHOGENIC 1 and negatively affect phytochrome B signalling

COLD REGULATED 27 and 28 are targets of CONSTITUTIVELY PHOTOMORPHOGENIC 1 and negatively affect phytochrome B signalling

Transcriptional Regulation of Photomorphogenesis in Brassica Napus Under Different Light Quality

Photomorphogenesis of the root system in developing sunflower seedlings: a role for sucrose

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