Molecular basis for RGD-containing peptides supporting adhesion and self-renewal of human pluripotent stem cells on synthetic surface

Design of chemically defined synthetic substrate surfaces for the in vitro maintenance of human pluripotent stem cells: A review

“…It is proved that the changes in amino acids surrounding the RGD sequence apparently affect the bioactivity of peptides, and removing the amino acids nearing aspartic acid (D) will eliminate the ability of peptides in supporting the culture of hPSCs. Finally, a new peptide with sequence of Ac‐KGGPQVTRGDTYRAY showed comparable performance to VN peptide 82 . In subsequent unpublished experiments, methods of molecular dynamics simulation and quartz crystal microbalance were applied to study the interaction between the RGD peptides and the corresponding integrin receptors.…”

“…Finally, a new peptide with sequence of Ac-KGGPQVTRGDTYRAY showed comparable performance to VN peptide. 82 In subsequent unpublished experiments, methods of molecular dynamics simulation and quartz crystal microbalance were applied to study the interaction between the RGD peptides and the corresponding integrin receptors. Surprisingly, we found that the ability of VN peptide to sustain the adhesion and self-renewal of hPSCs could be significantly improved by introducing mutations into the non-RGD sequences.…”

Thermoresponsive Hydrogels as Microniches for Growth and Controlled Release of Induced Pluripotent Stem Cells

“…For stimulating cell adhesion inside the microniche, cyclo (RGDfk), a cellular binding peptide sequence was integrated into the polymer network as a biochemical signal that support iPSCs' survival and proliferation (Figure 2d). [35,44,45] Finally, all of these precursors form the thermoresponsive hydrogel microniche (Figure 2e), of which the properties such as elasticity and precursor's contents can be further adjusted.…”

“…To stimulate cell adhesion inside the microniche, cyclo (-RGDfk), a cellular binding peptide sequence, was integrated into the polymer network as a biochemical signaling agent. [44,45] The concentration of the cyclo (-RGDfk) was adjusted to 1 mm, which was reported as the common reasonable concentration. [60] We embedded iPSCs (1 × 10 6 cells/mL) with precursors (dPG-BCN and pNIPAAm-co-PEG-N 3 , both of their concentration were 100 mg mL −1 , the ratio of them was 2:3, cyclo (RGDfk) 1mm) into the culture system (E 2 ≈ 357 Pa), to investigate the cell viability and expansion efficiency and overall to assess the performance of the artificial 3D niche environment in iPSCs' culture (Figure 7).…”

“…Additionally, rfRGD was introduced as biochemical signal to promote cell attachment. [35,44,45] After all the foremost parameters about precursor ratios (dPG-BCN [100 mg mL −1 ] and pNIPAAm-co-PEG-N 3 [100 mg mL −1 ]), elastic modulus (physical stiffness), [23,24] and cell seeding density were optimized, an optimal condition for cell culture was achieved. Therefore, the culture system could support iPSCs' fast expansion and maintain high quality about proliferation capacity and high pluripotency, which can be characterized by the test of Ki-67 staining, [46] alkaline phosphatase (ALP) staining, [47] and pluripotency immunostaining.…”