Molecular basis of macrolide-lincosamide-streptogramin (MLS) resistance in Finegoldia magna clinical isolates

Guérin, François; Lachaal, Sabrine; Auzou, Michel; Brun, Cécile; Barraud, Olivier; Decousser, Jean‐Winoc; Lienhard, Reto; Baraduc, R.; Dubreuil, L.; Cattoir, Vincent

doi:10.1016/j.anaerobe.2020.102220

Cited by 6 publications

(7 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…due to erm (A) subclass erm (TR), suggesting that these anaerobic members of the normal oropharyngeal microbiota may be an important reservoir of this gene for transfer to pathogens such as Streptococcus pyogenes [ 39 ]. In the second study, we detected 25 F. magna isolates (out of 69; i.e., 36%) that exhibited high-level MICs of erythromycin (>256 mg/L) harboring either erm (A) subclass erm (TR) or erm (B) [ 40 ]. Altogether, our findings confirm that erm (A) subclass erm (TR) is the predominant MLS resistance gene among GPAC and that erm (B) could also be detected whereas other erm -like genes have never been identified up to now.…”

Section: Discussionmentioning

confidence: 99%

“…It is mainly related to drug inactivation by nitroimidazole reductase encoded by nim genes [ 42 ]. To date, 11 nim genes ( nimA to nimK ) sharing between 54 and 90% amino acid identities have been described [ 40 ]. Of them, only nimB genes have been identified in the chromosome of some GPAC isolates ( P. anaerobius , A. prevotii , P. micra ) [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

In Vitro Antimicrobial Susceptibility Profiles of Gram-Positive Anaerobic Cocci Responsible for Human Invasive Infections

et al. 2021

Self Cite

View full text Add to dashboard Cite

The aim of this multicentre study was to determine the in vitro susceptibility to anti-anaerobic antibiotics of Gram-positive anaerobic cocci (GPAC) isolates responsible for invasive infections in humans. A total of 133 GPAC isolates were collected in nine French hospitals from 2016 to 2020. All strains were identified to the species level (MALDI-TOF mass spectrometry, 16S rRNA sequencing). Minimum inhibitory concentrations (MICs) of amoxicillin, piperacillin, cefotaxime, imipenem, clindamycin, vancomycin, linezolid, moxifloxacin, rifampicin, and metronidazole were determined by the reference agar dilution method. Main erm-like genes were detected by PCR. The 133 GPAC isolates were identified as follows: 10 Anaerococcus spp., 49 Finegoldia magna, 33 Parvimonas micra, 30 Peptoniphilus spp., and 11 Peptostreptococcus anaerobius. All isolates were susceptible to imipenem, vancomycin (except 3 P. micra), linezolid and metronidazole. All isolates were susceptible to amoxicillin and piperacillin, except for P. anaerobius (54% and 45% susceptibility only, respectively). MICs of cefotaxime widely varied while activity of rifampicin, and moxifloxacin was also variable. Concerning clindamycin, 31 were categorized as resistant (22 erm(A) subclass erm(TR), 7 erm(B), 1 both genes and 1 negative for tested erm genes) with MICs from 8 to >32 mg/L. Although GPACs are usually susceptible to drugs commonly used for the treatment of anaerobic infections, antimicrobial susceptibility should be evaluated in vitro.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

In Vitro Antimicrobial Susceptibility Profiles of Gram-Positive Anaerobic Cocci Responsible for Human Invasive Infections

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Immunosuppression and malnutrition are currently recognized causes of symbiotic bacteria [ 21 ]. Recent studies [ 22 ] have found that some virulence factors related to the pathogenicity of Finegoldia bacteria include the L protein, peptostreptococcal albumin binding protein (PAB), SufA, and Finegoldia bacteria. Bacteria have the ability to form biofilms, which can evade host immune defense and antimicrobial treatment [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

Analysis of Urinary Flora Characteristics in Urinary Tumor Based on 16S rRNA Sequence

Qiu

Liu

Zhong

et al. 2022

BioMed Research International

View full text Add to dashboard Cite

The relationship between urinary system tumors and urothelial microorganisms remains unexplored. This study is aimed at exploring the relationship between urinary flora and urinary tumors and identifying potential biomarkers for urinary tumors and new targets for prevention. We included four healthy adults (control group) and six patients diagnosed with urinary tract tumors (tumor group). In both groups, 10 and 50 ml clean middle urine samples were reserved. The 10 ml samples were analyzed (including pH, specific gravity, and leukocytes) using an automatic urine analyzer, and the 50 ml samples were analyzed by DNA extraction, 16S rRNA gene amplification, and high-throughput sequencing. The correlation between routine urine analysis and sequencing results was also analyzed. Testing using the DESeq2 method showed that, at the order level, there were significant differences in the abundance of Caulobacterales between the urinary flora of the two groups ( P < 0.05 ); family level, Bacteroidaceae, Actinomycetaceae, and Tsukamurellaceae ( P < 0.05 ); genus level, Finegoldia, Varibaculum, Actinobaculum, Propionimicrobium, Bacteroides, Brevundimonas, and Tsukamurella ( P < 0.05 ). LEfSe analysis found specific bacteria at the genus level in the urinary flora of the tumor group, namely, Finegoldia (genus Digestiflora) ( P < 0.001 ) and Varibaculum ( P < 0.001 ). Further correlation analysis showed that both species were positively correlated with the urine pH ( P < 0.05 ). PICRUSt analysis showed significant differences in the two functional pathways of cell transformation and metabolism ( P < 0.05 ). Combined with the results of bioinformatics analysis, some differential bacteria may be new biomarkers for urologic tumors, and there may be a correlation between urine pH and tumor occurrence. However, large-scale prospective studies and in vitro and in vivo experiments are required to further test and verify these findings.

show abstract

“…Reduced affinity to target molecule PBP alterations (Gajdacs et al, 2017) b-lactams target site mutation Methylation of the 23S rRNA: ermA, ermB, ermF, and ermTR (Reig et al, 2001;Guerin et al, 2020;Guerin et al, 2021) Nitroimidazole reductase: nimB (Theron et al, 2004) Macrolides Metronidazole…”

Section: Related Research Related Drugsmentioning

confidence: 99%

“…Certain GPAC species have developed resistance—for example, nim genes in Peptostreptococcus spp., possibly located on mobile genetic elements, encode nitroimidazole reductases responsible for drug inactivation ( Alauzet et al., 2019 ; Thomas and Gwenin, 2021 ). Until now, 11 nim genes ( nimA to nimK ) have been identified, but only the nimB gene has been found in the chromosome of certain GPAC isolates ( Peptostreptococcus anaerobius , Peptostreptococcus prevotii , Parvimonas micra , and Finegoldia magna ) ( Theron et al., 2004 ; Alauzet et al., 2019 ; Guerin et al., 2020 ).…”

Section: Antibiotic Resistance Of Several Prominent Toa Pathogensmentioning

confidence: 99%

Antibiotic Resistance and Mechanisms of Pathogenic Bacteria in Tubo-Ovarian Abscess

Tang

Zhou²,

Zhang³

2022

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

A tubo-ovarian abscess (TOA) is a common type of inflammatory lump in clinical practice. TOA is an important, life-threatening disease, and it has become more common in recent years, posing a major health risk to women. Broad-spectrum antimicrobial agents are necessary to cover the most likely pathogens because the pathogens that cause TOA are polymicrobial. However, the response rate of antibiotic treatment is about 70%, whereas one-third of patients have poor clinical consequences and they require drainage or surgery. Rising antimicrobial resistance serves as a significant reason for the unsatisfactory medical outcomes. It is important to study the antibiotic resistance mechanism of TOA pathogens in solving the problems of multi-drug resistant strains. This paper focuses on the most common pathogenic bacteria isolated from TOA specimens and discusses the emerging trends and epidemiology of resistant Escherichia coli, Bacteroides fragilis, and gram-positive anaerobic cocci. Besides that, new methods that aim to solve the antibiotic resistance of related pathogens are discussed, such as CRISPR, nanoparticles, bacteriophages, antimicrobial peptides, and pathogen-specific monoclonal antibodies. Through this review, we hope to reveal the current situation of antibiotic resistance of common TOA pathogens, relevant mechanisms, and possible antibacterial strategies, providing references for the clinical treatment of drug-resistant pathogens.

show abstract

Molecular basis of macrolide-lincosamide-streptogramin (MLS) resistance in Finegoldia magna clinical isolates

Cited by 6 publications

References 22 publications

In Vitro Antimicrobial Susceptibility Profiles of Gram-Positive Anaerobic Cocci Responsible for Human Invasive Infections

In Vitro Antimicrobial Susceptibility Profiles of Gram-Positive Anaerobic Cocci Responsible for Human Invasive Infections

Analysis of Urinary Flora Characteristics in Urinary Tumor Based on 16S rRNA Sequence

Antibiotic Resistance and Mechanisms of Pathogenic Bacteria in Tubo-Ovarian Abscess

Contact Info

Product

Resources

About