Molecular biological studies of adult and metacercarial stages of Petasiger exaeretus Dietz, 1909 (Digenea: Echinostomatidae)

Cech, Gábor; Molnár, Kálmán; Székely, Csaba

doi:10.1556/004.2017.020

Cited by 8 publications

(4 citation statements)

References 13 publications

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“…3), and were identical or nearly identical with the deposited sequences of P. phalacrocoracis (KY283999.1) described by Tkach, Kudlai [28], P. phalacrocoracis (KT956926.1) and Paryphostomum radiatum (KT956927.1) by Cech, Molnár [29]. Sequences of P. exaeretus with P. phalacrocoracis, Pa. radiatum did not match any of them and these differences can be attributed to host ecology and geography [29].…”

Section: Discussionsupporting

confidence: 51%

“…Petasiger exaeretus Dietz, 1909, is commonly found in birds belonging to the family Phalacrocoracidae [27]. The result obtained showed a 99% similarity to the partial 28S rDNA region of sporocyst, redia and cercaria sequences of P. exaeretus isolated from the Radix auricularia freshwater snail, recorded in Ukraine (KT956923.1) [28] and Hungary (KY284001.1-KY284007.1) [29] (Fig. 3), and were identical or nearly identical with the deposited sequences of P. phalacrocoracis (KY283999.1) described by Tkach, Kudlai [28], P. phalacrocoracis (KT956926.1) and Paryphostomum radiatum (KT956927.1) by Cech, Molnár [29].…”

Section: Discussionmentioning

confidence: 93%

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First molecular identification and phylogenetic tree of Petasiger exaeretus Dietz, 1909 (Digenea: Echinostomatidae) from an intermediate host Radix auricularia (L., 1758) in Greater Zab river, Iraq

Bashe

Ali

2019

Ann Agric Environ Med.

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Introduction. Radix auricularia (Linnaeus, 1758) is a freshwater gastropod belongs to the Lymnaeidae (pond snails) family which act as intermediate hosts or vectors of various parasitic flukes. No study has yet been undertaken on the prevalence of Petasiger spp. infection in R. auricularia. Species of Petasiger (Dietz, 1909) are a cosmopolitan parasite that utilize snails as the first intermediate host, with vertebrates like amphibians larvae and fish as the second intermediate host, followed by fish-eating birds. The current paper is considered to be the first report of Petasiger exaeretus parasitized R. auricularia in Iraq, which is supported with molecular and phylogenetic analysis. Materials and method. Freshwater snails R. auricularia were collected during October 2016-September 2017 from different locations of Sufaia village on the Greater Zab river, Erbil province, Iraq. Results. A total of 307 freshwater snails R. auricularia were collected, only five of them were infected with a prevalence of Petasiger exaeretus (1.62%). Conclusions. The current study agrees with the opinion of Selbach, Soldánová (26), which suggested the possibility of a much higher morphological diversity within Petasiger species, based on the number of described cercariae, compared with adult forms.It is clear that P. phalacrocoracis specimens have often been erroneously designated as P. exaeretus by many authors (Našincová et al., 1994). Certain morphological similarities and dissimilarities between P. exaeretus and P. phalacrocoracis can be detected: the pear-shaped body resembles P. exaeretus, whereas, P. phalacrocoracis have an elongated body.

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Section: Discussionsupporting

confidence: 51%

Section: Discussionmentioning

confidence: 93%

First molecular identification and phylogenetic tree of Petasiger exaeretus Dietz, 1909 (Digenea: Echinostomatidae) from an intermediate host Radix auricularia (L., 1758) in Greater Zab river, Iraq

Bashe

Ali

2019

Ann Agric Environ Med.

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“…Petasiger spp. are a commonly found trematode parasite in the intestine of piscivorous birds (particularly cormorants) in Europe, Asia, and Africa [ 48 , 76 ]. In Australia, Petasiger australis has been reported from Hoary-headed Grebe, Poliocephalus poliocephalus [ 71 ].…”

Section: Discussionmentioning

confidence: 99%

Parasites of Selected Freshwater Snails in the Eastern Murray Darling Basin, Australia

Barton

Zhu

Nuhoglu

et al. 2022

IJERPH

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Aquatic snails serve an important role in the ecosystem. They also play an essential role in the life cycle of many parasites as hosts and may pose risks to animal and human health. In Australia, the role of snails in the transmission of parasites of livestock is well studied. However, despite the country’s unique biodiversity and wildlife, little is known about the role of snails in the transmission and survival of parasites in other ecosystems, including aquatic and aquaculture systems. This study aimed to determine the occurrence of parasites in freshwater snails in the eastern Murray Darling Basin. A total of 275 snails were collected from various localities, including aquaculture fishery ponds and natural creeks during the summer and autumn months in the southern hemisphere. Three different species of freshwater snails, all common to the area, were found, including Bullastra lessoni (n = 11), Isidorella hainesii (n = 157), and Haitia acuta (n = 107), of which 9.1%, 1.3%, and 4.7%, respectively, were found to be harboring various developmental stages of Trematoda. No other parasite was found in the examined snails. Parasites were identified as Choanocotyle hobbsi, Plagiorchis sp. and Petasiger sp. based on the sequences of their ITS2, 18S, and 28S ribosomal DNA region. Herein, we report a native parasite Choanocotyle hobbsi in an introduced snail, Haitia acuta, from both natural and aquaculture ponds. As there are no genetic sequences for adult specimens of Petasiger spp. and Plagiorchis spp. collected in Australia for comparison, whether the specimens collected in this study are the larval stage of one of the previously described species or are a new, undescribed species cannot yet be determined. Our results also suggest snails collected from aquaculture ponds may be infected with considerably more parasites.

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“…The introduction of DNA sequencing has accelerated the elucidation of life cycles of trematodes [9,10], including representative species of the superfamily Echinostomatoidea Looss, 1902 [11][12][13][14][15][16][17]. However, no sequence data from Rhopalias spp.…”

Section: Introductionmentioning

confidence: 99%

Elucidating the life cycle of opossum parasites: DNA sequences reveal the involvement of planorbid snails as intermediate hosts of Rhopalias spp. (Trematoda: Echinostomatidae) in Brazil

et al. 2023

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Echinostomatid digeneans belonging to the genus Rhopalias are intestinal trematodes found mainly in opossums in the New World. The genus comprises seven species, but their life cycles and intermediate hosts have been unknown until now. During our long-term study carried out in freshwater habitats within the state of Minas Gerais, Southeast Brazil, echinostomatid cercariae lacking collar spines were found in planorbid snails Biomphalaria glabrata, Biomphalaria straminea, Drepanotrema lucidum and Gundlachia ticaga in six different batches of snail samples collected between 2010 and 2019. Morphologically, the larvae reported herein are morphologically consistent with each other and characterized by the presence of 2–3 large ovoid or spherical corpuscles in each main duct of the excretory system, resembling to Cercaria macrogranulosa previously described from the same region of Brazil. Partial sequences of the ITS (ITS1-5.8S-ITS2) region and 28S gene of the nuclear ribosomal RNA operon, and partial sequences of mitochondrial nad1 and cox1 genes were obtained and compared with the data available for members of the family Echinostomatidae. Nuclear markers indicate that all samples of cercariae evaluated in the present study can be assigned to Rhopalias, but distinct from North American isolates of Rhopalias macracanthus, Rhopalias coronatus and Rhopalias oochi (divergence 0.2–1.2% in 28S and 0.8–4.7% in ITS). The lack of differences verified in both 28S and ITS in 5 out 6 studied samples suggested that they belong to the same species. However, nad1 sequences revealed that our cercariae correspond to three distinct species of Rhopalias (interspecific divergence: 7.7–9.9%), named here as Rhopalias sp. 1, found in B. straminea and G. ticaga, Rhopalias sp. 2 found in B. glabrata and D. lucidum, and Rhopalias sp. 3 also found in D. lucidum. They also differ by 10.8–17.2% from a North American isolate of R. macracanthus sequenced in this study. The cox1 sequences obtained for Rhopalias sp. 1 and Rhopalias sp. 2 (but not Rhopalias sp. 3) reveal that they are distinct from North American isolates of R. macracanthus (genetic divergence 16.3–16.5% and 15.6–15.7%, respectively), R. coronatus (9.2–9.3% and 9.3–9.5%) and Rhopalias oochi (9.0% and 9.5–10.1%). Encysted metacercariae with general morphology similar to that of the body of cercariae were found in tadpoles of Rhinella sp. from the same stream where snails harbored Rhopalias sp. 2, suggesting that the amphibians could act as second intermediate hosts of species of Rhopalias. Data obtained provide the first insights into the life cycle of this unusual echinostomatid genus.

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Molecular biological studies of adult and metacercarial stages of Petasiger exaeretus Dietz, 1909 (Digenea: Echinostomatidae)

Cited by 8 publications

References 13 publications

First molecular identification and phylogenetic tree of Petasiger exaeretus Dietz, 1909 (Digenea: Echinostomatidae) from an intermediate host Radix auricularia (L., 1758) in Greater Zab river, Iraq

First molecular identification and phylogenetic tree of Petasiger exaeretus Dietz, 1909 (Digenea: Echinostomatidae) from an intermediate host Radix auricularia (L., 1758) in Greater Zab river, Iraq

Parasites of Selected Freshwater Snails in the Eastern Murray Darling Basin, Australia

Elucidating the life cycle of opossum parasites: DNA sequences reveal the involvement of planorbid snails as intermediate hosts of Rhopalias spp. (Trematoda: Echinostomatidae) in Brazil

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