Molecular Biology in Glioblastoma Multiforme Treatment

Abbruzzese, Claudia; Persico, Michele; Matteoni, Silvia; Paggi, Marco G.

doi:10.3390/cells11111850

Cited by 11 publications

(5 citation statements)

References 18 publications

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“…Consequently, the role of CXCL5 in GBM remains only partially understood, and the associated target molecules await further elucidation. Additionally, given the complexity of tumor mechanisms, our choice of models and initial exploration of mechanisms may not have fully addressed the multifaceted nature of GBM [ 25 – 27 ]. Therefore, future research endeavors should prioritize mechanistic experiments to unveil the intricacies of immune infiltration patterns and elucidate CXCL5's role in drug development.…”

Section: Discussionmentioning

confidence: 99%

Prognostic marker CXCL5 in glioblastoma polyformis and its mechanism of immune invasion

Yu,

Zhou,

Niu

et al. 2024

BMC Cancer

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Glioblastoma multiforme (GBM) is the most aggressive brain cancer with a poor prognosis. Therefore, the correlative molecular markers and molecular mechanisms should be explored to assess the occurrence and treatment of glioma.WB and qPCR assays were used to detect the expression of CXCL5 in human GBM tissues. The relationship between CXCL5 expression and clinicopathological features was evaluated using logistic regression analysis, Wilcoxon symbolic rank test, and Kruskal–Wallis test. Univariate, multivariate Cox regression and Kaplan–Meier methods were used to assess CXCL5 and other prognostic factors of GBM. Gene set enrichment analysis (GSEA) was used to identify pathways associated with CXCL5. The correlation between CXCL5 and tumor immunoinfiltration was investigated using single sample gene set enrichment analysis (ssGSEA) of TCGA data. Cell experiments and mouse subcutaneous transplanted tumor models were used to evaluate the role of CXCL5 in GBM. WB, qPCR, immunofluorescence, and immunohistochemical assays showed that CXCL5 expression was increased in human GBM tissues. Furthermore, high CXCL5 expression was closely related to poor disease-specific survival and overall survival of GBM patients. The ssGSEA suggested that CXCL5 is closely related to the cell cycle and immune response through PPAR signaling pathway. GSEA also showed that CXCL5 expression was positively correlated with macrophage cell infiltration level and negatively correlated with cytotoxic cell infiltration level. CXCL5 may be associated with the prognosis and immunoinfiltration of GBM.

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Section: Discussionmentioning

confidence: 99%

Prognostic marker CXCL5 in glioblastoma polyformis and its mechanism of immune invasion

Yu,

Zhou,

Niu

et al. 2024

BMC Cancer

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“…Despite the implementation of aggressive multidisciplinary interventions, GBM continues to manifest dismal prognostic outcomes. Typically, patients encounter a median progression-free survival (PFS) of merely 6-7 months, coupled with an overall survival (OS) spanning 14-16 months from the point of initial diagnosis [1][2][3].…”

Section: Introductionmentioning

confidence: 99%

Recurrence Patterns after Radiotherapy for Glioblastoma with [(11)C]methionine Positron Emission Tomography-Guided Irradiation for Target Volume Optimization

Debreczeni-Máté,

Törő,

Simon

et al. 2024

Diagnostics

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11C methionine (11C-MET) is increasingly being used in addition to contrast-enhanced MRI to plan for radiotherapy of patients with glioblastomas. This study aimed to assess the recurrence pattern quantitatively. Glioblastoma patients undergoing 11C-MET PET examination before primary radiotherapy from 2018 to 2023 were included in the analysis. A clinical target volume was manually created and fused with MRI-based gross tumor volumes and MET PET-based biological target volume. The recurrence was noted as an area of contrast enhancement on the first MRI scan, which showed progression. The recurrent tumor was identified on the radiological MR images in terms of recurrent tumor volume, and recurrences were classified as central, in-field, marginal, or ex-field tumors. We then compared the MET-PET-defined biological target volume with the MRI-defined recurrent tumor volume regarding spatial overlap (the Dice coefficient) and the Hausdorff distance. Most recurrences occurred locally within the primary tumor area (64.8%). The mean Hausdorff distance was 39.4 mm (SD 32.25), and the mean Dice coefficient was 0.30 (SD 0.22). In patients with glioblastoma, the analysis of the recurrence pattern has been mainly based on FET-PET. Our study confirms that the recurrence pattern after gross tumor volume-based treatment contoured by MET-PET is consistent with the FET-PET-based treatment described in the literature.

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“…[ 1 ] High proliferation and invasion activity as well as the heterogeneous appearance of the tumor are responsible for its malignancy. [ 2 ] The interaction with its tumor microenvironment (TME) is of particular importance for tumor proliferation, e.g., it was shown that tumor cells take advantage of the interactions with other cell types in the brain. [ 3,4 ] GBM´s TME is composed of microglia, neurons, astrocytes, and vascular cells.…”

Section: Introductionmentioning

confidence: 99%

Primary Glial Cell and Glioblastoma Morphology in Cocultures Depends on Scaffold Design and Hydrogel Composition

et al. 2023

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Abstract3D cell cultures better replicate the in vivo environment compared to 2D models. Glioblastoma multiforme, a malignant brain tumor, highly profits from its cellular environment. Here, the U87 glioblastoma cell line in the presence/absence of primary astrocytes is studied. Thiolated hyaluronic acid (HA‐SH) hydrogel reinforced with microfiber scaffolds is compared to Matrigel. Hyaluronic acid is a major extracellular matrix (ECM) component in the brain. Poly(ɛ‐caprolactone) (PCL) scaffolds are written by meltelectrowriting in a box and triangular shaped design with pore sizes of 200 µm. Scaffolds are composed of 10‐layers of PCL microfibers. It is found that scaffold design has an impact on cellular morphology in the absence of hydrogel. Moreover, the used hydrogels have profound influences on cellular morphology resulting in spheroid formation in HA‐SH for both the tumor‐derived cell line and astrocytes, while cell viability is high. Although cocultures of U87 and astrocytes exhibit cell–cell interactions, polynucleated spheroid formation is still present for U87 cells in HA‐SH. Locally restricted ECM production or inability to secrete ECM proteins may underlie the observed cell morphologies. Thus, the 3D reinforced PCL‐HA‐SH composite with glioma‐like cells and astrocytes constitutes a reproducible system to further investigate the impact of hydrogel modifications on cellular behavior and development.

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Molecular Biology in Glioblastoma Multiforme Treatment

Cited by 11 publications

References 18 publications

Prognostic marker CXCL5 in glioblastoma polyformis and its mechanism of immune invasion

Prognostic marker CXCL5 in glioblastoma polyformis and its mechanism of immune invasion

Recurrence Patterns after Radiotherapy for Glioblastoma with [(11)C]methionine Positron Emission Tomography-Guided Irradiation for Target Volume Optimization

Primary Glial Cell and Glioblastoma Morphology in Cocultures Depends on Scaffold Design and Hydrogel Composition

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