Molecular biology of extended-spectrum β-lactamase-producing Enterobacteriaceae responsible for digestive tract colonization

Moustaoui, Najat; Soukri, Abdelaziz; Elmdaghri, Naima; Boudouma, Mohammed; Benbachir, M.

doi:10.1016/j.jhin.2004.01.007

Cited by 6 publications

(5 citation statements)

References 27 publications

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“…In the current study, plasmid profiling is of limited epidemiological value since we found only 12 plasmid positive isolates of the 25 isolates extracted. Compare with plasmid profile among clinical isolates, our finding is in agreement with those found in Morocco (Moustaoui, Soukri, Elmdaghri, Boudouma, & Benbachir, 2004), but it is in contrast with those found in Brazil (Lopes et al, 2005). Among the 25 isolates examined, 93% of the plasmids identified were low molecular weight plasmids as reported previously (Eisen et al, 1995), while high molecular weight plasmid (35.8 MDa) was found in only 17% of the plasmid positive isolates.…”

Section: Resultssupporting

confidence: 91%

Incidence of Klebsiella pneumonia in street foods sold in Malaysia and their characterization by antibiotic resistance, plasmid profiling, and RAPD–PCR analysis

Haryani

Noorzaleha²,

Fatimah

et al. 2007

Food Control

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A total of 78 samples of street foods from different states in Malaysia were examined for the presence of Klebsiella pneumonia. K. pneumonia contamination was recorded in 32% of the samples examined. The frequency of the K. pneumonia isolates containing plasmids was shown to be 48%. Susceptibility testing showed that all isolates were highly resistant to ampicillin (100%), erythromycin (100%), rifampicin (100%), streptomycin (96%) and sulfamethoxazole (80%), but susceptible to chloramphenicol. RAPD proved to be the most effective technique in discriminating the K. pneumonia isolates since dendrogram constructed using the combination of 4 primers could differentiate 25 isolates of K. pneumonia to 25 strains. This is the first report that revealed the occurrence of K. pneumonia in street foods sold in Malaysia.

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Section: Resultssupporting

confidence: 91%

Incidence of Klebsiella pneumonia in street foods sold in Malaysia and their characterization by antibiotic resistance, plasmid profiling, and RAPD–PCR analysis

Haryani

Noorzaleha²,

Fatimah

et al. 2007

Food Control

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“…The use of standardized PFGE protocols allows for rapid comparison of DNA fingerprints between different laboratories to enhance disease surveillance. However, laboratories that use PFGE to analyze K. pneumoniae cannot compare their results because the protocols differ from each other in critical parameters, especially the restriction enzymes and electrophoresis conditions [14,15,16], so there is a need for an optimized PFGE protocol for K. pneumoniae subtyping which can readily be implemented in different laboratories for data interpretation.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Pulse-Field Gel Electrophoresis for Subtyping of Klebsiella pneumoniae

Han

Zhou

et al. 2013

IJERPH

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A total of 110 strains of Klebsiella pneumoniae were used to optimize pulsed-field gel electrophoresis (PFGE) for subtyping of K. pneumoniae. For optimization of electrophoresis parameters (EPs) of XbaI-PFGE, 11 isolates were analyzed with XbaI digestion using three EPs. The EP of a switch time of 6 to 36 s for 18.5 h gave clearest patterns and was declared the optimal EP for XbaI PFGE of K. pneumoniae. By software analysis and pilot study, AvrII was chosen as another PFGE enzyme. Both XbaI- and AvrII-PFGE gave D-values higher than 0.99 for 69 K. pneumoniae isolated from different sources. Our results also showed good typeability, reproducibility of both XbaI- and AvrII-PFGE for K. pneumoniae subtyping. Furthermore, the established PFGE method also had good discriminatory power to distinguish outbreak K. pneumoniae strains and a high degree of consistency with multilocus sequence typing method. A rapid PFGE protocol was established here, which could be used for genotyping and other researches of K. pneumoniae.

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“…Se observa que la E. coli tiene una mayor presencia en foco gastrointestinal y urinario, mientras que la K. pneumoniae, a pesar de que ha sido asociada a diferentes focos primarios, se asoció a cuadros clínicos de origen respiratorio. Se describe en la literatura que ciertas condiciones del huésped tales como múltiples vías de acceso y mecanismo de traslocación bacteriana, facilitan la infección por este tipo de patógenos (13)(14)(15). También, la distribución de gérmenes detectados en este estudio (K. pneumoniae: 45.4 %) es comparable a la de otros reportes.…”

Section: Discussionunclassified

Bacteriemia por enterobacterias productores de β-lactamasas de espectro extendido, en una unidad de cuidado intensivo pediátrico: serie de casos

et al. 2021

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Introducción: la prevalencia de bacteriemias causadas por gérmenes productores de β-lactamasas de espectro extendido en niños ha aumentado a nivel mundial, al igual que sus complicaciones y costos. Objetivo: caracterizar la infección por enterobacterias productoras de β-lactamasas de espectro extendido en la población pediátrica hospitalizada en unidad de cuidado intensivo. Métodos: se llevó a cabo un estudio descriptivo retrospectivo, identificando pacientes hospitalizados en una unidad de cuidado intensivo pediátrico con reporte de hemocultivos, durante enero de 2016 hasta diciembre de 2019. Resultados: se revisaron 1 638 historias. En once casos (0.67 %) se evidencio al menos un cultivo positivo para enterobacterias productoras de β-lactamasas de espectro extendido. Ninguna infección fue asociada al cuidado de la salud. Todos los sujetos tuvieron fiebre, con una temperatura mediana de 39°C (Rango intercuartílico de 0.2). El germen aislado con mayor frecuencia fue E. coli, seguido de K. pneumoniae. Los reactantes de fase aguda como la proteína C reactiva, la velocidad de sedimentación globular y la procalcitonina presentaban valores por encima del límite superior normal. El tratamiento antibiótico fue pertinente en la totalidad de los casos y solo se requirió cambio de antibiótico en un caso por no respuesta. Conclusiones: este estudio reunió datos locales que permiten sentar la línea de base de esta condición. Se generaron recomendaciones clínicas adicionales que pueden mejorar los desenlaces en población pediátrica.

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Molecular biology of extended-spectrum β-lactamase-producing Enterobacteriaceae responsible for digestive tract colonization

Cited by 6 publications

References 27 publications

Incidence of Klebsiella pneumonia in street foods sold in Malaysia and their characterization by antibiotic resistance, plasmid profiling, and RAPD–PCR analysis

Incidence of Klebsiella pneumonia in street foods sold in Malaysia and their characterization by antibiotic resistance, plasmid profiling, and RAPD–PCR analysis

Optimization of Pulse-Field Gel Electrophoresis for Subtyping of Klebsiella pneumoniae

Bacteriemia por enterobacterias productores de β-lactamasas de espectro extendido, en una unidad de cuidado intensivo pediátrico: serie de casos

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