Molecular Biology of Potyviruses

Revers, Frédéric; Garcı́a, Juan Antonio

doi:10.1016/bs.aivir.2014.11.006

Cited by 498 publications

(363 citation statements)

References 554 publications

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“…For all interspecific recombinants described so far, including SuWMV, the genetic exchange concerns the Nterminal part of the P1 coding region. This region shows the highest diversity in length and sequence among potyviruses (Revers & Garcia, 2015). It is one of the least constrained regions of the potyvirus genome (Moury et al, 2006) and is not required for virus viability (Revers & Garcia, 2015).…”

Section: Geographic Distribution and Evolution Of Prsv-like Virusesmentioning

confidence: 99%

“…This region shows the highest diversity in length and sequence among potyviruses (Revers & Garcia, 2015). It is one of the least constrained regions of the potyvirus genome (Moury et al, 2006) and is not required for virus viability (Revers & Garcia, 2015). Since it is self-cleaved during proteolytic maturation, it does not require a compatibility of its cleavage site with the rest of the genome.…”

Section: Geographic Distribution and Evolution Of Prsv-like Virusesmentioning

confidence: 99%

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New species in the papaya ringspot virus cluster: Insights into the evolution of the PRSV lineage

Desbiez¹,

Wipf-Scheibel²,

Millot³

et al. 2017

Virus Research

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Please cite this article as: Desbiez, C., Wipf-Scheibel, C., Millot, P., Verdin, E., Dafalla, G., Lecoq, H., New species in the papaya ringspot virus cluster: insights into the evolution of the PRSV lineage.Virus Research http://dx.doi.org/10. 1016/j.virusres.2017.06.022 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. SuWMV appeared 10 times more frequent than WMVBV in that country (14.6% vs. 1.5% of the samples tested). The geographic structure and molecular diversity patterns of the putative and acknowledged species suggest that the PRSV-like cluster originated in the Old World about 3600 years ago, with an important diversification in Africa.

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Section: Geographic Distribution and Evolution Of Prsv-like Virusesmentioning

confidence: 99%

Section: Geographic Distribution and Evolution Of Prsv-like Virusesmentioning

confidence: 99%

New species in the papaya ringspot virus cluster: Insights into the evolution of the PRSV lineage

Desbiez¹,

Wipf-Scheibel²,

Millot³

et al. 2017

Virus Research

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“…Potyviruses are characterized by the genome-linked protein VPg covalently attached to the 5¢-terminus of viral genomic RNA (Revers & García, 2015). VPg can interact with a virus-encoded protein HC-Pro; this interaction is believed to result in the formation of virion terminal structures, which are distinguishable by election and atomic force microscopy and can be labelled with HC-Pro antibodies (Torrance et al, 2006).…”

Section: Role Of Virions In Cell-to-cell Transport Of Filamentous Virmentioning

confidence: 99%

“…VPg can interact with a virus-encoded protein HC-Pro; this interaction is believed to result in the formation of virion terminal structures, which are distinguishable by election and atomic force microscopy and can be labelled with HC-Pro antibodies (Torrance et al, 2006). Moreover, a sub-population of particles isolated from plants infected with Potato virus A has been shown to contain the viral CI (cylindrical inclusion) protein localized to one end of the virion (GabrenaiteVerkhovskaya et al, 2008), presumably due to the reported ability of CI to interact with HC-Pro (Revers & García, 2015). The CI protein is targeted to plasmodesmata-associated sites by the recently discovered CI-interacting potyvirus protein P3N-PIPO, which is essential for viral transport and is capable of directed intracellular trafficking to plasmodesmata and translocation through plasmodesmata to neighbouring cells (Chung et al, 2008;Vijayapalani et al, 2012).…”

Section: Role Of Virions In Cell-to-cell Transport Of Filamentous Virmentioning

confidence: 99%

Helical capsids of plant viruses: architecture with structural lability

Solovyev

Makarov

2016

Journal of General Virology

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Capsids of numerous filamentous and rod-shaped plant viruses possess helical symmetry. In positive-stranded RNA viruses, helical capsids are typically composed of many identical subunits of the viral capsid protein (CP), encapsidating a molecule of viral genomic RNA. Current progress in structural studies of helical plant viruses has revealed differences between filamentous and rod-shaped viruses, both in structural folds of their CPs and in the interactions of CP molecules in their capsids. Many filamentous and rod-shaped viruses have functionally similar lateral inter-subunit contacts on the outer virion surface. Additionally, the extreme Nterminal CP region in filamentous viruses is intrinsically disordered. Taken together, the available data establish a link between the structural features of molecular interactions of CP molecules and the physical properties of helical virions ranging from rigidity to flexibility. Overall, the structure of helical plant viruses is significantly more labile than previously thought, often allowing structural transitions, remodelling and the existence of alternative structural forms of virions. These properties of virions are believed to be functionally significant at certain stages of the viral life cycle, such as during translational activation and cell-to-cell transport. In this review, we discuss structural and functional features of filamentous and rod-shaped virions, highlight their shared features and differences, and lay emphasis on the relationships between the molecular structure of viral capsids and their properties including virion shape, lability and capability of structural remodelling.

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“…The TEV genome is composed by a (þ)ssRNA molecule of 9539 nucleotides that encodes for 11 multifunctional peptides [26]. For such a genome length, the size of the adaptive landscape is 4 9539 genotypes; within which, only 64 correspond to combinations of the six mutations that made TEV-At17 different from its tobacco-adapted antecessor [24].…”

Section: Introductionmentioning

confidence: 99%

Efficient escape from local optima in a highly rugged fitness landscape by evolving RNA virus populations

2016

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Predicting viral evolution has proven to be a particularly difficult task, mainly owing to our incomplete knowledge of some of the fundamental principles that drive it. Recently, valuable information has been provided about mutation and recombination rates, the role of genetic drift and the distribution of mutational, epistatic and pleiotropic fitness effects. However, information about the topography of virus' adaptive landscapes is still scarce, and to our knowledge no data has been reported so far on how its ruggedness may condition virus' evolvability. Here, we show that populations of an RNA virus move efficiently on a rugged landscape and scape from the basin of attraction of a local optimum. We have evolved a set of Tobacco etch virus genotypes located at increasing distances from a local adaptive optimum in a highly rugged fitness landscape, and we observed that few evolved lineages remained trapped in the local optimum, while many others explored distant regions of the landscape. Most of the diversification in fitness among the evolved lineages was explained by adaptation, while historical contingency and chance events contribution was less important. Our results demonstrate that the ruggedness of adaptive landscapes is not an impediment for RNA viruses to efficiently explore remote parts of it.

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Molecular Biology of Potyviruses

Cited by 498 publications

References 554 publications

New species in the papaya ringspot virus cluster: Insights into the evolution of the PRSV lineage

New species in the papaya ringspot virus cluster: Insights into the evolution of the PRSV lineage

Helical capsids of plant viruses: architecture with structural lability

Efficient escape from local optima in a highly rugged fitness landscape by evolving RNA virus populations

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