Molecular characterisation and mechanisms of resistance of multidrug-resistant human serovar Typhimurium isolated in Amiens (France)

Biendo, M.; Laurans, G.; Thomas, D.; Canarelli, B.; Hamdad-Daoudi, Farida; Rousseau, Florence; Castelain, Sandrine; Eb, F.

doi:10.1016/j.ijantimicag.2005.05.003

Cited by 15 publications

(16 citation statements)

References 30 publications

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“…Genes coding for p-AmpC ␤-lactamases (CIT, DHA, ACC, EBC, MOX, and FOX) and bla OXA-1 were characterized by PCR amplification with specific primers and sequencing (2,23,25) in all isolates. E. coli isolates that had negative results for p-AmpC ␤-lactamases but that displayed a resistance phenotype consistent with AmpC production on the basis of their resistance to AMC and cefoxitin and inhibition with phenyl boronic acid and cloxacillin (cefotetan/cefotetan-cloxacillin Etest; AB bioMérieux, Solna, Sweden) were categorized as c-AmpC.…”

Section: Studymentioning

confidence: 99%

Spanish Multicenter Study of the Epidemiology and Mechanisms of Amoxicillin-Clavulanate Resistance in Escherichia coli

Ortega

Oteo

Zaldumbide

et al. 2012

Antimicrob Agents Chemother

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mWe conducted a prospective multicenter study in Spain to characterize the mechanisms of resistance to amoxicillin-clavulanate (AMC) in Escherichia coli. Up to 44 AMC-resistant E. coli isolates (MIC > 32/16 g/ml) were collected at each of the seven participant hospitals. Resistance mechanisms were characterized by PCR and sequencing. Molecular epidemiology was studied by pulsed-field gel electrophoresis (PFGE) and by multilocus sequence typing. Overall AMC resistance was 9.3%. The resistance mechanisms detected in the 257 AMC-resistant isolates were OXA-1 production (26.1%), hyperproduction of penicillinase (22.6%), production of plasmidic AmpC (19.5%), hyperproduction of chromosomic AmpC Escherichia coli is an important etiologic agent for both nosocomial-and community-acquired infections in humans (9, 13, 22). Amoxicillin-clavulanate (AMC) is one of the most widely used antibiotics in many countries (3,11,15). In Spain, a 34.7% increase in community use of AMC was recorded from 2000 to 2006 (20). Recently, blood isolates of E. coli nonsusceptible to AMC increased from 9.3% (2003) Enzymatic mechanisms of E. coli resistance to AMC include hyperproduction of plasmid-mediated class A ␤-lactamases such as TEM-1 and SHV-1 (18, 31), plasmid-mediated AmpC-type ␤-lactamase (p-AmpC) (21), chromosomal AmpC ␤-lactamase (c-AmpC) (21), production of inhibitor-resistant TEM (IRT) ␤-lactamases (17, 27), plasmid-mediated ␤-lactamase OXA-1 (32), and complex mutant TEM (CMT) enzymes than combine IRT-and extended-spectrum ␤-lactamase (ESBL)-type substitutions (26).In spite of the significant increase in AMC use in the last several years, there is little recent information available about the prevalence of AMC resistance mechanisms in E. coli; most previous studies analyzed strains isolated more than 10 years ago from single hospitals in the United States (12, 28), France (16), and Spain (19,24). Accordingly, the aim of this prospective Spanish national multicenter study was to investigate the epidemiology and mechanisms of AMC resistance in clinical isolates of E. coli causing both community and nosocomial infections. MATERIALS AND METHODS Study design and bacterial isolates.A prospective multicenter study was designed to obtain E. coli isolates resistant to AMC (MIC of Ն 32/16 g/ml and/or disk inhibition zone of Յ13 mm according to the Clinical and Laboratory Standards Institute [CLSI]) (7) from clinical samples col-

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Section: Studymentioning

confidence: 99%

Spanish Multicenter Study of the Epidemiology and Mechanisms of Amoxicillin-Clavulanate Resistance in Escherichia coli

Ortega

Oteo

Zaldumbide

et al. 2012

Antimicrob Agents Chemother

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“…The drug resistance spectra of MDR strains of Salmonella serovars have also been expanding in recent years, especially for the aminopenicillines, tetracyclines, sulfonamides and chloramphenicol, and often more than 50% of S. Typhimurium strains isolated in France are multidrug resistant [13].…”

Section: Introductionmentioning

confidence: 99%

Prevalence and antibiotic-resistance of Salmonella isolated from food in Morocco

Bouchrif

Paglietti

Murgia

et al. 2009

J Infect Dev Ctries

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Background: Salmonellosis remains one of the most frequent food-borne diseases worldwide, especially in developing countries. The emergence of antimicrobial resistance in Salmonella isolates from food can potentially compromise the treatment of these infections. This investigation was conducted for the first time in Morocco both to detect the occurrence of Salmonella in foods as well as to determine the antibiotic resistance profile of the Salmonella isolates. Methodology: In total, 11,516 food samples collected from 2002 to 2005 were investigated. Isolated Salmonella were characterized by serotyping and susceptibilities were determined for 15 antimicrobial drugs using the disc diffusion assay. Results: The overall percentage of Salmonella prevalence (n=105) was 0.91% with rates of 71% for slaughterhouses and 9% for seafood. Sixteen different serotypes were identified among 104 Salmonella enterica isolates including serotypes Infantis (n=25), Bredeney (n=13), Blokley (n=11), Typhimurium (n=9), Mbandaka (n=8), Branderup II (n=7), and Kiambu (n=6); 1 isolate of Salmonella enterica belonged to subspecies II salamae. Twenty-nine percent of isolates (n=30/105) were resistant to at least one antimicrobial. Resistance to tetracycline was the most common finding (21%), followed by resistance to ampicillin (13%), amoxicillin+clavulanic acid (9%), streptomycin (7%), chloramphenicol (4%) and nalidixic acid (3,8%). None of the isolates was resistant to 3rd-cephalosporin and fluoroquinolones (i.e. ciprofloxacin). Multidrug resistance (MDR) was seen in 9.5% of the isolates, mainly in S. Typhimurium DT104 with R-type ACSSuT and S. Hadar. Conclusions: Despite a low frequency of Salmonella isolation, S. Typhimurium DT104 was identified in the first step of the food chain. The study points out the need control antibiotic resistance in Salmonella isolated from food in Morocco to avoid the spread of MDR.

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“…Production of enzymes due to extended-spectrum cephalosporinases has been observed in S. Choleraesuis resistance, and most of the isolates in this study were found to display this property with cephalothin (Table 3) [51]. This is an indication that the isolates could be harboring cefotaximehydrolyzing β-lactamases (CTX-M types) or CMY-2 AmpC β-lactamase that could hydrolyze cephalosporins normally transferred through conjugative plasmids, transposons, or integrons [52,53]. This is supported by the fact that resistance to β-lactams in S. enterica is primarily caused by the production of acquired β-lactamases [54], with TEM-1, PSE-1, and OXA-1 having been described as the enzymes most frequently related to ampicillin and amoxicillin/clavulanate resistance.…”

Section: Discussionmentioning

confidence: 74%

“…Other authors [50,51] have reported in Taiwan the existence of S. Choleraesuis that is resistant to multiple antibiotics, including ciprofloxacin and ceftriaxone, through various mechanisms. Apart from the different mechanisms -production of hydrolytic enzymes to destroy the antibiotic surrounding the environment, mutations in the specific genes to escape from the action of antibiotics, decreased outer membrane permeability to prevent antibiotics from entering the bacterial cell itself, and efflux systems to exclude antibiotics before they become effective -other mechanisms have been described [51,52]. Production of enzymes due to extended-spectrum cephalosporinases has been observed in S. Choleraesuis resistance, and most of the isolates in this study were found to display this property with cephalothin (Table 3) [51].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial profile of Salmonella enterica serotype Choleraesuis from free-range swine in Kakamega fish market, western Kenya

Onyango

Ndeda

Wandili

et al. 2014

J Infect Dev Ctries

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Introduction: Salmonella enterica subspecies enterica serovar Choleraesuis is a host-adapted, facultative, intracellular pathogen that causes swine paratyphoid. Its antimicrobial resistance presents a challenge to feed manufacturing industries. However, stopping antibiotics in animal feed would have economic implications for the industry. Methodology: Conventional microbial methods for isolation and identification of S. Choleraesuis were employed. The isolates were subjected to screening against 17 antimicrobial agents and genotyping of resistance markers by PCR. The data were then analyzed and presented in percentages. Results: Phenotypically, 43 out of 95 isolates showed multidrug resistance. Among the 17 antibiotics tested, resistance was observed as follows: sulphonamides (45

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Molecular characterisation and mechanisms of resistance of multidrug-resistant human serovar Typhimurium isolated in Amiens (France)

Cited by 15 publications

References 30 publications

Spanish Multicenter Study of the Epidemiology and Mechanisms of Amoxicillin-Clavulanate Resistance in Escherichia coli

Spanish Multicenter Study of the Epidemiology and Mechanisms of Amoxicillin-Clavulanate Resistance in Escherichia coli

Prevalence and antibiotic-resistance of Salmonella isolated from food in Morocco

Antimicrobial profile of Salmonella enterica serotype Choleraesuis from free-range swine in Kakamega fish market, western Kenya

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