Molecular characterisation and phylogenetic position of Hemicycliophora labiata Colbran, 1960, from turfgrass in Korea, with comments on its morphology

Abstract

SummaryHemicycliophora labiata was reported from the rhizosphere of Poa pratensis and for the first time in Korea. Females and juveniles are molecularly characterised and morphological and morphometric descriptions supplied. Species identification was made using an integrative approach considering morphological characteristics and the results of the analyses of the D2-D3 expansion segment of 28S rRNA, ITS1-5.8S-ITS2 of rRNA and the partial COI gene sequences. The phylogenetic position of H. labiata is resolved after recent misidentifications due to the existence of a wide morphological plasticity within the genus. Females and juveniles from Korea conform to the original description and also to subsequent species descriptions from Australia, South Africa, Sri Lanka, and India. Despite the close morphological similarities with H. typica de Man, 1921, the two species can be adequately differentiated based on molecular data. This is the first molecular characterisation of H. labiata. The use of molecular markers in species delimitation continues to explore the existence of species complexes, thus providing a better understanding of the unresolved species biodiversity within this speciose genus.

Hemicycliophora ahvasiensis n. sp. (Nematoda: Hemicycliophoridae), and data on a known species, from Iran

“…9, H. labiata , H. epicharoides , H. typica and H. dulli . The close affinity of the aforementioned species was already observed ( Subbotin et al, 2014 ; Van den Berg et al, 2018 ; Maria et al, 2018 ; Mwamula et al, 2020 ).…”

Occurrence of Plant-Parasitic Nematodes of Turfgrass in Korea

“…Twenty-eight species/taxa belonging to 16 genera and 12 families of PPNs were identified and described from the 781 sod cores collected from the 13 golf courses distributed across Korea. Comprehensive morphological and molecular characterization of various studied taxa, including new species description were presented in the earlier published articles (Mwamula et al, 2020a(Mwamula et al, , 2020b(Mwamula et al, , 2020c(Mwamula et al, , 2020d. Among the 28 species/taxa identified, 12 species/taxa (Tylenchorhynchus thermophilus, Pratylenchus diversity indices are presented in Table 3.…”

“…Twenty-eight species/taxa belonging to 16 genera and 12 families of PPNs were identified and described from the 781 sod cores collected from the 13 golf courses distributed across Korea. Comprehensive morphological and molecular characterization of various studied taxa, including new species description were presented in the earlier published articles ( Mwamula et al, 2020a , 2020b , 2020c , 2020d , 2020e , 2020f ). Among the 28 species/taxa identified, 12 species/taxa ( Tylenchorhynchus thermophilus , Pratylenchus zeae , Mesocriconema nebraskense , Mesocriconema sp., Neopsilenchus sp., Hemicycliophora labiata , Helicotylenchus caudatus , Helicotylenchus microlobus , Helicotylenchus asiaticus , Filenchus sp., Meloidogyne graminicola , and Paralongidorus koreanensis ) are reported as new records in Korea.…”

“…Measurements and micrographs were taken using a Zeiss imager Z2 microscope (Carl Zeiss, Gottingen, Germany) fitted with Axio-vision software: Material Science Software for Research and Engineering software (Carl Zeiss). The various promorphs/genera were characterized to species level according to diagnostic criteria using morphological, morphometric and molecular tools as detailed in Mwamula et al (2020 , 2020b , 2020c , 2020d , 2020e , 2020f ). Promorphs were corresponded to the identified species as described by Vandenbossche et al (2011) .…”

“…Thus, correct species identification plus an understanding of their diversity on turfgrass is a prerequisite for a successful nematode control and management strategy. A survey of Korean golf courses in April 2017 to 2019 uncovered several species of various suborders of PPNs; as detailed by Mwamula et al (2020a , 2020b , 2020c , 2020d , 2020e , 2020f ). However, it is evident that little is known about their diversity on turfgrass in Korea.…”