Molecular characterisation of a putative Atlantic salmon (Salmo salar) odorant receptor

Wickens, Andrew P.; May, Denise; Rand‐Weaver, Mariann

doi:10.1016/s1096-4959(01)00364-5

Cited by 21 publications

(24 citation statements)

References 36 publications

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“…Two types of degenerated primers were used for the PCR amplifications (Yasuoka et al, 1999, 7 Asano- Miyoshi et al, 2000, Irie-Kushiyama et al, 2004, Wickens et al, 2001, with nested polymerase chain reaction (PCR) to amplify a 490 bp fragment from lacustrine sockeye salmon olfactory mRNA. The following 'hot start' thermal cycling parameters were used; 95℃ for 3 min and 80℃ for 1 min (1 cycle); addition of TaKaRa Ex Taq (TaKaRa, Shiga, Japan); 95℃ for 1 min, 55℃ for 1 min and 72℃ for 1 min (35 cycles); followed by a final DNA extension of 72 ℃ for 10 min.…”

Section: Sample Collectionmentioning

confidence: 99%

“…In database searches, OR genes were picked up including catfish (Ngai et al, 1993a), goldfish (Cao et al, 1998), Atlantic salmon (Wickens et al, 2001), Japanese loach 10 (Irie- Kushiyama et al, 2004) and zebrafish (Alioto et al, 2005). These amino acid sequence data included various OR subfamilies, and were given temporary names following previous study (Irie-Kushiyama et al, 2004).…”

Section: Phylogenetic Analysismentioning

confidence: 99%

“…Although previous studies have suggested that salmon ORs in the peripheral olfactory epithelium play an important role in salmonid homing migration, only a few putative MOR genes in Atlantic salmon have been identified to date (Wickens et al, 2001;Dukes et al, 2004Dukes et al, , 2006. In this study, we isolated OR genes from five salmonid species: lacustrine sockeye salmon (Oncorhynchus nerka), pink salmon (O. gorbuscha), chum salmon (O. keta), masu salmon (O. masou) and rainbow trout (O. mykiss), in an effort to examine both their molecular characteristics and phylogeny among these salmonid species.…”

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confidence: 99%

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Cloning and characterization of an odorant receptor in five Pacific salmon

Morinishi

Shiga

Suzuki

et al. 2007

Comparative Biochemistry and Physiology Part B: Biochemistry an

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The olfactory system of fish is extremely important as it is able to recognize and distinguish a vast of odorous molecules that are involved in wide ranges of behaviors including reproduction, homing, kin recognition, feeding and predator avoidance; all of which are paramount for their survival. We cloned and characterized one type olfactory receptors (ORs) from five salmonids:keta), masu salmon (O. masou) and rainbow trout (O. mykiss). Lacustrine sockeye salmon olfactory receptor 1 (LSSOR1) showed high sequence homology to the OR subfamily, and was expressed only in the olfactory epithelium (as indicated by PCR amplified genomic DNA and cDNA). OR genes from the five salmonids examined all showed strong homology (96-99%) to each other. Hypervariable regions, believed to be ligand-binding pockets, showed homologous completely matched amino acid sequences except for one amino acid in pink salmon olfactory receptor 1 (PSOR1), revealing that these ORs may be well conserved among salmon species.These results suggest that the isolated 5 salmonid ORs might play an important role in salmon life cycles.

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Section: Sample Collectionmentioning

confidence: 99%

Section: Phylogenetic Analysismentioning

confidence: 99%

mentioning

confidence: 99%

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Cloning and characterization of an odorant receptor in five Pacific salmon

Morinishi

Shiga

Suzuki

et al. 2007

Comparative Biochemistry and Physiology Part B: Biochemistry an

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“…There are two types of OR genes, main olfactory receptors (MORs), which are expressed in ciliated olfactory receptor cells; and vomeronasal olfactory receptors (VORs), which are expressed in microvillous olfactory receptor cells. MOR genes have also been identified in a number of salmonids (Wickens et al 2001;Dukes et al 2004Dukes et al , 2006Morinishi et al 2007). The olfactory receptor gene expression was investigated in different life stages of Atlantic salmon, demonstrating that seven V2R-like (OlfC) genes were expressed at higher levels in juveniles (parr and smolts) than in adults (Johnstone et al 2011(Johnstone et al , 2012.…”

Section: -5 Biochemical and Molecular Biological Studies On Pacificmentioning

confidence: 99%

Physiological Mechanisms of Imprinting and Homing Migration of Pacific Salmon

Ueda¹

2016

Aqua-BioSci. Monogr. (ABSM)

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“…Recently, an odorant receptor (ASOR1; Atlantic salmon odorant receptor 1) has been first isolated from Atlantic salmon (Wickens et al, 2001). Besides, Dukes et al (2004) reported the odorant receptor gene (SORB; salmon olfactory receptor B) expression changes during the parr-smolt transformation in salmon.…”

Section: Introductionmentioning

confidence: 99%

Identification of an olfactory imprinting-related gene in the lacustrine sockeye salmon, Oncorhynchus nerka

et al. 2007

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The olfactory system of salmonids is essential for imprinting and the subsequent homing migration. Olfactory imprinting-related genes were identified in the olfactory system of 1-and 3-year-old lacustrine sockeye salmon (Oncorhynchus nerka) using a subtractive hybridization technique of representational difference analysis (cDNA-RDA). We have obtained a partial clone from a subtractive cDNA library of 1-year-old fish that contains a 756 bp open reading frame coding for a putative protein with 252 amino acid residues named the Sockeye salmon Olfactory system Imprinting related Gene (SOIG). By northern hybridization, the SOIG mRNA was only expressed in the olfactory epithelium and not in other tissues. In situ hybridization showed that the expression of SOIG mRNA was observed in the olfactory receptor cells and basal cells of the olfactory epithelium. This suggests that SOIG could have specific and important roles in olfactory system.

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Molecular characterisation of a putative Atlantic salmon (Salmo salar) odorant receptor

Cited by 21 publications

References 36 publications

Cloning and characterization of an odorant receptor in five Pacific salmon

Cloning and characterization of an odorant receptor in five Pacific salmon

Physiological Mechanisms of Imprinting and Homing Migration of Pacific Salmon

Identification of an olfactory imprinting-related gene in the lacustrine sockeye salmon, Oncorhynchus nerka

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