Molecular characterisation of equid alphaherpesvirus 1 strains isolated from aborted fetuses in Poland

Matczuk, Anna Karolina; Skarbek, Małgorzata; Jackulak, Natalia; Bażanów, Barbara

doi:10.1186/s12985-018-1093-5

Cited by 9 publications

(17 citation statements)

References 25 publications

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“…All the viruses described in the current report belonged to the ORF30 N 752 genotype, consistent with the predominance of the N 752 variants among EHV-1 abortion cases in Poland [ 25 , 57 ] and other countries [ 22 , 28 , 58 ]. While data from a recent investigation in Ireland [ 27 ] suggested that viruses with D 752 genotype were more likely not only to induce neurological disease, but also to be associated with multiple abortion cases as opposed to sporadic abortions, our results underscore the fact that infection with viruses of either N 752 or D 752 genotype should be considered equally important, and similar precaution/control measures should be implemented to minimise the economic losses due to disease outcomes associated with these infections.…”

Section: Discussionsupporting

confidence: 88%

Outbreak of equid herpesvirus 1 abortions at the Arabian stud in Poland

2020

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Background Equid herpesvirus 1 (EHV-1) infections are endemic worldwide, including Poland. Many are subclinical, but some are associated with respiratory disease, abortion, neonatal foal death, or neurological disease. We describe an outbreak of abortions in Arabian mares at a well-managed State stud farm in Poland. Case presentation Eight of 30 pregnant mares aborted and one gave birth to a weak foal that died within 72 h after birth. EHV-1 was isolated from all fetuses as well as from the diseased foal. All viruses belonged to the N752 variant based on the predicted open reading frame (ORF) 30 amino acid sequence. All were identical to each other and to previous EHV-1 viruses from the same stud based on the ORF68 sequence analysis. The outbreak coincided with the lapse in the routine yearly EHV-1/4 vaccinations of the mares. Conclusions Multiple abortion due to EHV-1 infection can occur in well-managed groups of horses. Reactivation of latent EHV-1 in one of the resident mares followed by a horizontal spread was considered the most likely explanation for the outbreak. Routine vaccination is an important part of a herd-heath program.

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Section: Discussionsupporting

confidence: 88%

Outbreak of equid herpesvirus 1 abortions at the Arabian stud in Poland

2020

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“…Thirty-four out of 269 isolated in Ireland between 1990 and 2017 showed N752D [12], and only two out of 56 EHV-1 isolated from aborted fetuses in India had the neuropathogenic marker [8]. The N752D genotype was not found in EHV-1 isolated in Brazil [23], in Turkey [24] and in the 27 strains isolated in Poland between 1993 and 2017 [11]. Even in other countries the prevalence of the neuropathogenic genotype was rather low, as in Japan (2.7%), the USA (10.8–19.4%), Argentina (7.4%), France (24%), and Germany (10.6%) [25,26,27,28,29,30].…”

Section: Discussionmentioning

confidence: 99%

“…Sequencing and analysis of the ORF68 gene are widely used for grouping EHV-1 [4,7,10,11]. An extensive study on ORF68 gene of EHV-1 isolated worldwide has been recently carried out [12].…”

Section: Discussionmentioning

confidence: 99%

“…An extensive study on ORF68 gene of EHV-1 isolated worldwide has been recently carried out [12]. After that six groups have been originally proposed [4], further SNPs have been described and new groups have been proposed [7,10,11]. All ORF68 sequences detected in this study were not included in any group because they showed a 118 bp deletion in the nucleotide sequence 701–818 that have been not observed in any existing group.…”

Section: Discussionmentioning

confidence: 99%

“…In particular, a region of about 600 bp in ORF68 was particularly polymorphic, and therefore it was tentatively adopted as a marker system for efficiently grouping the isolates into six groups [4]. This method was subsequently used for comparing isolates from different geographical regions [7,8,9,10,11,12]. Recently a Multi-locus analysis approach, based on sequencing heterologous regions in 26 open reading frames, proved a more comprehensive method of strain typing than only ORF68 sequencing.…”

Section: Introductionmentioning

confidence: 99%

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Equid alphaherpesvirus 1 from Italian Horses: Evaluation of the Variability of the ORF30, ORF33, ORF34 and ORF68 Genes

Preziuso

Sgorbini

Marmorini

et al. 2019

Viruses

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Equid alphaherpesvirus 1 (EHV-1) is an important pathogen of horses. It is spread worldwide and causes significant economic losses. The ORF33 gene has a conserved region that is often used as target in diagnostic PCR protocols. Single nucleotide point (SNP) mutations in ORF30 are usually used to distinguish between neuropathogenic and non-neuropathogenic genotypes. An ORF68 SNP-based scheme has been used for grouping different isolates. Recently, the highest number of variable sites in EHV-1 from the UK has been found in ORF34. In this study, EHV-1 positive samples from Italian horses with a history of abortion were investigated by amplifying and sequencing the ORF30, ORF33, ORF34 and ORF68 genes. Most animals were infected by the neuropathogenic type A2254G. A 118 bp deletion was found at nucleotide positions 701–818 of the ORF68 gene, making impossible to assign the samples to a known group. Sequencing of the ORF34 gene with a newly designed nested PCR showed new SNPs. Analysis of these sequences and of those obtained from genetic databases allowed the identification of at least 12 groups. These data add depth to the knowledge of EHV-1 genotypes circulating in Italy.

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AK‐2011 strain for the development of a vaccine against equine rhinopneumonitis

Abisheva¹,

Abishov²,

Khairullaeva³

et al. 2022

Transbounding Emerging Dis

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Equine rhinopneumonitis is an acute, highly contagious disease found virtually worldwide. The purpose of the studies presented in this paper is to develop a technology for the manufacture of a cell-derived equine rhinopneumonitis vaccine, as well as to assess the safety and immunogenicity of the newly developed vaccine in laboratory animals model. The object of the studies was the AK-2011 strain isolated from the horses suffering from rhinopneumonitis during an outbreak of abortions. The viability of the AK-2011 strain was assessed using a continuous line of calf trachea cells, a continuous line of calf kidney cells, a continuous line of sheep kidney cells, a continuous line of bovine kidney cells, a continuous line of green monkey kidney cells, a continuous line of Syrian hamster kidney cells, a primary trypsinized culture of horse kidney cells grown in tubes and flasks and the AK-2011 laboratory strain of equine rhinopneumonitis virus with biological activity of 6.0 lg TCID 50/cm 3 . Sequencing and polymerase chain reaction analysis were performed. The virus isolated from the ORF68 gene in Kazakhstan appeared to be the most similar to the T-953 and 2222-03 strains isolated in the USA and Australia, respectively, in terms of phylogenetics. As to primary infections, cytopathic effects (CPEs) induced by the AK-2011 virus stain (dilution 10 1 ) in calf trachea and horse kidney cell cultures were stable from the first to tenth passages, with biological activity of 5.75-6.00 lg TCID 50/cm 3 . CPEs caused by the virus were apparent on days 2-3, further developed intensively and extended to 60-80% of the cell monolayer on days 5-7. The vaccine results can be used to immunize horses on farms against rhinopneumonia, and horses should be immunized twice with an interval of 2-3 months.

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Molecular characterisation of equid alphaherpesvirus 1 strains isolated from aborted fetuses in Poland

Cited by 9 publications

References 25 publications

Outbreak of equid herpesvirus 1 abortions at the Arabian stud in Poland

Outbreak of equid herpesvirus 1 abortions at the Arabian stud in Poland

Equid alphaherpesvirus 1 from Italian Horses: Evaluation of the Variability of the ORF30, ORF33, ORF34 and ORF68 Genes

AK‐2011 strain for the development of a vaccine against equine rhinopneumonitis

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