Molecular characterisation of the Ancylostoma-secreted protein family from the adult stage of Ancylostoma caninum

Zhan, Bin; Liu, Yueyuan; Badamchian, Mahnaz; Williamson, Angela; Feng, Jia; Loukas, Alex; Hawdon, John M.; Hotez, Peter J.

doi:10.1016/s0020-7519(03)00111-5

Cited by 92 publications

(84 citation statements)

References 38 publications

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“…In H. polygyrus and Ancylostoma caninum SCP/TAPS molecules are on (and closely below) the parasite surface, as well as associated with the parasite's gut and oesophagus. This is consistent with these molecules having a role in immunomodulating the host, but also consistent with interacting with the host more widely [27,36]. More generally, related molecules occur in a wide variety of non-parasitic species, perhaps suggesting that the conserved features of these proteins are simply domains that can be usefully deployed in many settings [27].…”

Section: Comparing the Genomes Of Parasitic And Free-living Nematodessupporting

confidence: 69%

The genomic basis of nematode parasitism

Viney¹

2017

Briefings in Functional Genomics

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Nematodes are highly abundant animals, and many species have a parasitic lifestyle. Nematode parasites are important pathogens of humans and other animals, and there is considerable interest in understanding their molecular and genomic adaptations to nematode parasitism. This has been approached in three main ways: comparing the genomes of closely related parasitic and free-living taxa, comparing the gene expression of parasitic and free-living life cycle stages of parasitic nematode species, and analysing the molecules that parasitic nematodes excrete and secrete. To date, these studies show that many species of parasitic nematodes have genomes that have large gene families coding for proteases/peptidases, protease inhibitors, SCP/TAPS proteins and acetylcholinesterases, and in many cases there is evidence that these appear to be used by parasitic stages inside hosts, and are often secreted. Many parasitic nematodes have taxa-restricted gene families that also appear to be involved in parasitism, emphasizing that there is still much to be discovered about what it takes to be a parasitic nematode.

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Section: Comparing the Genomes Of Parasitic And Free-living Nematodessupporting

confidence: 69%

The genomic basis of nematode parasitism

Viney¹

2017

Briefings in Functional Genomics

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“…To date, several immunomodulatory molecules from adult hookworms have been characterized, including a T-cell apoptotic factor (15) and an NK binding ligand (26) among others (7). In addition, Ancylostoma hookworms were shown previously to release a tissue inhibitor of metalloproteases in abundance (48). Mammalian tissue inhibitor of metalloproteases were show previously to stimulate the host cell production of IL-10 (23).…”

Section: Discussionmentioning

confidence: 99%

Host Cytokine Production, Lymphoproliferation, and Antibody Responses during the Course of Ancylostoma ceylanicum Infection in the Golden Syrian Hamster

Méndez

Valenzuela

et al. 2005

Infect Immun

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The Syrian Golden hamster (Mesocricetus auratus) has been used to model infections with the hookworm Ancylostoma ceylanicum. New molecular immunological reagents to measure cellular immune responses in hamsters were developed and used to determine the impact of A. ceylanicum hookworm infection on host cytokine responses and lymphoproliferation. Initial larval infection with 100 third-stage A. ceylanicum larvae resulted in predominant Th1 responses (upregulation of proinflammatory cytokines) that lasted for the duration of larval migration and continued up to 14 days postinfection (prepatency). Subsequently, development of larvae into egg-laying adult hookworms (patency) coincided with a switch to Th2 predominant responses (interleukin-4 [IL-4]) as well as a marked increase in IL-10 production. This switch also concurred with reduced host lymphoproliferative responses to hookworm antigens. The findings demonstrate a similarity in immune responses between hamsters and humans infected with hookworms, suggesting that hamsters will be a useful animal model species for examining host immunity to human hookworm infections.Hookworm infection is one of the most prevalent parasitic diseases infecting over 700 million people in tropical and subtropical regions of the world (16). Clinical symptoms of human hookworm infection include iron-deficiency anemia (18) and protein-losing enteropathy (14) that may lead to physical, mental and cognitive growth retardation effects (25,42,43). Although chemotherapy is effective at eliminating existing adult parasites, reinfection can occur rapidly after treatment (1, 38). This observation, together with the unique epidemiology of hookworm infection, in which the worm burdens increase with age in endemic regions (6), have led to the suggestion that hookworms can either evade or suppress host immune responses (28, 35). Therefore, characterization of the immune response to hookworm in animal models would constitute a valuable tool to understand the underlying immunological mechanisms of human hookworm infections.The Syrian Golden hamster (Mesocricetus auratus) has been developed as a model to study hookworm infection caused by the dog and cat hookworm, Ancylostoma ceylanicum. A. ceylanicum is also a minor cause of human hookworm infection in parts of Asia (11). Unlike mice, which do not permit the development of adult hookworms (12, 41), hamsters are permissive hosts for the hookworm A. ceylanicum (19,24,32,40,46). In most studies to date, hamsters have been infected with A. ceylanicum third-stage infective larvae (L3) via oral gavage. As the human hookworm A. duodenale is both orally infective as well as infective through the percutaneous route, this approximates a natural route of infection. More importantly, this model reproduces weight and blood loss that result from human infection with A. duodenale.In addition to modeling the host-parasite relationship for human hookworm infection, the hamsters have been used with some success for investigating protective immunity from a new generati...

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“…Zhan and the colleagues reported that RT-PCR analysis of six ASPs from A. caninum indicated an exclusive expression of Ac-asp-1 and Ac-asp-2 by the L3 stage, in contrast to Ac-asp-5 and Ac-asp-6, which were expressed in adult worms only. On the other hand, Acasp-3 and Ac-asp-4 were expressed in both life stages of A. caninum (Zhan et al 2003). Our analysis of mRNA expression of novel A. ceylanicum ASPs showed that Ace-asp-3, Ace-asp-4 and Ace-asp-5 were present in all three stages (L3, ssL3 and adult).…”

Section: Discussionmentioning

confidence: 65%

“…A common attribute of all ASPs is the presence of a SCP domain (Zhan et al 1999(Zhan et al , 2003, which is found in many phylogenetically diverse proteins including vespid venom allergens (King and Sprangford 2000), mammalian sperm proteins (Giese et al 2002), free-living Ceanohabditis elegans proteins and plant pathogenesis proteins (Szyperski et al 1998). It is known that single-SCP-domain ASPs are more widespread among nematodes than double-domain forms (Zhan et al 1999.…”

Section: Discussionmentioning

confidence: 99%

Cloning and molecular characterization of cDNAs encoding three Ancylostoma ceylanicum secreted proteins

Bąska

Daniłowicz-Luebert

Januszkiewicz

et al. 2013

Acta Parasitologica

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Ancylostoma ceylanicum belongs to a group of soil-transmitted helminths, which infect almost 576 mln people worldwide and are a major cause of anaemia and malnutrition. Upon contact with a permissive host, third-stage larvae (L3) residing in the environment become activated larvae (ssL3), a process associated with changes in the profile of gene expression. Ancylostoma secreted proteins (ASPs) are the major proteins secreted during larvae activation and play a crucial role in hookworm adaptation to parasitism. Here we report the cloning using RACE-PCR technique of three novel ASPs from the hookworm A. ceylanicum (Ace-asp-3, Ace-asp-4, and Ace-asp-5) and computational analysis of the protein sequences. All three proteins contain SCP (Sperm Coating Protein) domain characteristic for previously described ASP proteins. Real-time PCR analysis shows significant up-regulation of Ace-asp-3 and Ace-asp-5 expression in adult worms and correlated down-regulation in ssL3 larvae. On the other hand, expression of Ace-asp-4 was increased in ssL3 stages and decreased in adult parasites.

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Molecular characterisation of the Ancylostoma-secreted protein family from the adult stage of Ancylostoma caninum

Cited by 92 publications

References 38 publications

The genomic basis of nematode parasitism

The genomic basis of nematode parasitism

Host Cytokine Production, Lymphoproliferation, and Antibody Responses during the Course of Ancylostoma ceylanicum Infection in the Golden Syrian Hamster

Cloning and molecular characterization of cDNAs encoding three Ancylostoma ceylanicum secreted proteins

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