Molecular Characteristics and Virulence Gene Analysis of Non-O157 Shiga Toxin–Producing <i>Escherichia coli</i> from Cattle in Xinjiang

Tong, Panpan; Zhang, Ling; Xie, Jinxin; Zhang, Mengmeng; Tang, Xuelin; Liu, Yingyu; Xia, Lining; Su, Zhanqiang

doi:10.1089/fpd.2021.0020

Cited by 3 publications

(5 citation statements)

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“…Surprisingly, the prevalence of cattle STEC in this area was 12.9%, which was far lower than the ones in other countries (27.4%–53%) (Lambertini et al 2015; Ballem et al 2020; Thierry et al 2020). Compared with the international published data, the prevalence of cattle STEC in China was relatively low (3%–12.8%) (Wu 2014; Tong et al 2021). Feed, sampling season, age, detecting methods (Peng et al 2019), farm sizes, geographical location, or farm sanitation might explain these differences.…”

Section: Discussionmentioning

confidence: 84%

“…We found that none of the serotypes belonged to the STEC big seven serotypes (O157, O26, O45, O103, O111, O121, and O145). In China, non‐big seven STEC from livestock occupy a dominant position (Peng et al 2019; Tong et al 2021). However, the most prevalent serogroups were O6, O155, and O21, which were not consistent with other regions in China (Peng et al 2019).…”

Section: Discussionmentioning

confidence: 99%

“…The only cattle STEC possessed stx1a subtype. The previous study indicated that stx1a was the only subtype present in all stx1-positive cattle STEC of China (Tong et al 2021). Given the few samples, further studies related to cattle faecal samples are needed to explore the prevalence of virulence genes.…”

Section: Discussionmentioning

confidence: 99%

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Genetic and phylogenetic characterization of Shiga toxin-producing Escherichia coli and enteropathogenic E. coli from livestock in Jiangsu by using whole-genome sequencing

Yue-hong

Liu

Han

et al. 2022

Journal of Applied Microbiology

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Aims: There are knowledge gaps regarding STEC and EPEC strains in livestock in Jiangsu, China. This study aimed to evaluate the potential public health significance of STEC and EPEC strains isolated from livestock by determining the serotypes, virulence profiles, and genetic relationship with international STEC strains. Methods and Results:A total of 68 STEC and 37 EPEC strains were obtained from 231 faecal sheep samples and 70 faecal cattle samples. By using whole-genome sequencing (WGS) analysis, all STEC belonged to 15 O: H serotypes, and the most prevalent serotypes were O6:H10 (19.1%), O155:H21 (14.7%), and O21:H25 (10.3%). The main Shiga toxin gene subtypes detected were stx1c (41.2%), stx1a (26.5%), stx2b (14.7%), and stx2k (14.7%). Only the STEC from cattle carried eae gene. Other adherence-associated or toxin-related genes, including lpfA (70.6%), iha (48.5%), subA (54.4%), and ehxA (33.8%), were found in STEC. All EPEC strains were bfpA-negative, and the predominant eae variants were eae-β1 (62.2%), eae-ζ (21.6%), and eae-θ (8.1%). The core-genome multilocus sequence typing (cgMLST) analysis revealed nine scattered clusters in STEC and one dominant cluster in EPEC. The strains with the same serotypes, including O22:H8 and O43:H2 in the two towns, possessed a closely genomic distance. The core genome single-nucleotide polymorphism (cgSNP) showed that part of STEC strains in this study was clustered with isolates possessing the same serotypes from the Netherlands, Sweden, and Xinjiang of China. Five serotypes of STEC isolates were associated with the clinical STEC strains from databases. Conclusion:This study provided the diverse serotypes and the virulence genes profiles in STEC and EPEC strains. Local strains possessed widely diverse and scattered clusters by cgMLST. Closely genomic correlation with clinical isolates displayed that part of the STEC strains may threaten to public health.Significance and Impact of the Study: Non-O157 STEC strains act as important pathogens for human infections. This study supports the increased surveillance work of non-O157 STEC rather than just O157 STEC in this region.

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Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

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Genetic and phylogenetic characterization of Shiga toxin-producing Escherichia coli and enteropathogenic E. coli from livestock in Jiangsu by using whole-genome sequencing

Yue-hong

Liu

Han

et al. 2022

Journal of Applied Microbiology

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“… E. coli that harbour stx1a, stx2a and/or stx2c have been isolated from cattle and sheep [11–13]. STEC strains producing stx1c , stx1d and/or stx2b have been associated with sheep and goats [13–16], stx2e have a porcine animal reservoir [17], and E.…”

Section: Introductionmentioning

confidence: 99%

“…Although a wide variety of stx subtypes are found in ruminants and many different animal hosts, previous studies have indicated that certain stx subtypes may be associated with specific animal reservoirs. E. coli that harbour stx1a, stx2a and/or stx2c have been isolated from cattle and sheep [11][12][13]. STEC strains producing stx1c, stx1d and/or stx2b have been associated with sheep and goats [13][14][15][16], stx2e have a porcine animal reservoir [17], and E. coli harbouring stx2g have been isolated from cattle [18].…”

Section: Introductionmentioning

confidence: 99%

Escherichia coli encoding Shiga toxin subtype Stx2f causing human infections in England, 2015–2022

Den Ouden,

Greig,

Rodwell

et al. 2023

Journal of Medical Microbiology

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Introduction. Shiga toxin-producing Escherichia coli (STEC) belong to a diverse group of gastrointestinal pathogens defined by the presence of Shiga toxin genes (stx) of which there are at least ten subtypes (Stx1a-Stx1d and Stx2a-Stx2g). Gap Statement. Initially thought to be associated with mild symptoms, more recently STEC encoding stx2f have been isolated from cases of haemolytic uraemic syndrome (HUS) and the clinical significance and public health burden require further investigation. Aim. We analysed clinical outcomes and genome-sequencing data linked to patients infected with STEC encoding-stx2f in England to assess the risk to public health. Methodology. One hundred and twelve E. coli (n=58 isolates encoded stx2f; n=54 isolates E. coli belonging to CC122 or CC722 that had eae but were negative for stx) isolated from patients' faecal specimens between 2015 and 2022 were genome sequenced and linked to epidemiological and clinical outcome data. All isolates were investigated for the presence of virulence genes and a maximum-likelihood phylogeny of isolates belonging to CC122 and CC722 was constructed. Results. There were 52 cases infected with STEC harbouring stx2f between 2015 and 2022, with the majority identified in 2022. Most cases resided in the North of England (n=39/52, 75 %), were female (n=31, 59.6 %) and/or aged five and under (n=29, 55.8 %). Clinical outcome data were available for 40/52 cases (76.9 %) and 7/40(17.5 %) were diagnosed with STEC-HUS. In the two most common clonal complexes, CC122 and CC722, the presence of the stx2f-encoding prophage correlated with the presence of additional virulence genes, astA, bfpA and cdt, located on an 85kbp IncFIB plasmid. Conclusions. Certain serotypes of E. coli harbouring stx2f cause severe clinical outcomes, including STEC-HUS. Public health advice and possible interventions are limited, as little is known about the animal and environmental reservoirs and transmission routes. We recommend more comprehensive and standardized collection of microbiological and epidemiological data, and routine sharing of sequencing data between public health agencies worldwide.

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Antibiotic Resistance and Genomic Analysis of Shiga Toxin–Producing Escherichia coli from Dairy Cattle, Raw Milk, and Farm Environment in Shaanxi Province, China

Zhang,

Liu,

Sheng

et al. 2024

Foodborne Pathogens and Disease

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Molecular Characteristics and Virulence Gene Analysis of Non-O157 Shiga Toxin–Producing Escherichia coli from Cattle in Xinjiang

Cited by 3 publications

References 28 publications

Genetic and phylogenetic characterization of Shiga toxin-producing Escherichia coli and enteropathogenic E. coli from livestock in Jiangsu by using whole-genome sequencing

Genetic and phylogenetic characterization of Shiga toxin-producing Escherichia coli and enteropathogenic E. coli from livestock in Jiangsu by using whole-genome sequencing

Escherichia coli encoding Shiga toxin subtype Stx2f causing human infections in England, 2015–2022

Antibiotic Resistance and Genomic Analysis of Shiga Toxin–Producing Escherichia coli from Dairy Cattle, Raw Milk, and Farm Environment in Shaanxi Province, China

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