Molecular Characterization and Antibiotic Resistance Profiling of<i>Salmonella</i>Isolated from Retail Turkey Meat Products

Fakhr, Mohamed K.; Sherwood, Julie S.; Thorsness, Jessica L.

doi:10.1089/fpd.2006.3.366

Cited by 18 publications

(13 citation statements)

References 35 publications

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“…Different prevalence rates of Salmonella spp. in food, especially poultry and poultry products, have been reported by different authors [12][13][14][15][16][17]. Salmonella contamination rates observed in our country are relatively high (22.92%) and confirm the widespread contamination of pork and chicken meat with Salmonella spp.…”

Section: Discussionsupporting

confidence: 84%

First study of Salmonella in meat in Romania

Mihaiu

Lapusan

Tanasuica

et al. 2014

J Infect Dev Ctries

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Introduction: The increasing antimicrobial resistance of Salmonella isolates is of major public health concern, but information regarding these aspects is still lacking in Romania. This study focused on a detailed and accurate investigation concerning prevalence, serotypes, and antimicrobial resistance patterns of Salmonella strains, isolated from pork and chicken meat, collected from all regions of Romania in 2011. Methodology: The research was conducted on 650 samples of chicken and pork meat collected from production units and retail markets located in various regions of Romania. A total of 149 Salmonella isolates were recovered (22.92%), serotyped, confirmed by PCR, and further tested for antimicrobial susceptibility. Results: Thirteen Salmonella serovars were identified; predominant serovars included Infantis, Typhimurium, Derby and Colindale. Multiple resistance was found in 83.22% (n = 124) of the isolates. The isolates were frequently resistant to tetracycline (80.53%), streptomycin (81.21%), sulfamethoxazole (87.25%), nalidixic acid (65.10%), and ciprofloxacin (42.95%). Additionally, a markedly lower resistance rate was observed for ampicillin (20.81%), chloramphenicol (16.78%), and ceftazidime (11.41%). Among 137 resistant Salmonella isolates, 35 different resistance patterns were found. Conclusion: A high prevalence of Salmonella spp. and a relatively high resistance rate to multiple antimicrobials was found. This data indicates that chicken and pork meat could constitute a source of human exposure to multidrug-resistant Salmonella and therefore could be considered a potential vehicle of resistant Salmonella foodborne diseases. Further actions are needed to succesfully implement a national surveillance program for better monitoring of these resistant pathogens.

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Section: Discussionsupporting

confidence: 84%

First study of Salmonella in meat in Romania

Mihaiu

Lapusan

Tanasuica

et al. 2014

J Infect Dev Ctries

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“…4:12: nonmotile and S . Reading were recovered from retail turkey meat samples in North Dakota, USA [10]. Khaitsa et al [12] reported six Salmonella serotypes from 959 turkey products as follows: Hadar, Heidelberg, Typhimurium var.…”

Section: Discussionmentioning

confidence: 99%

“…The results of these studies showed that prevalence and serotype distribution of Salmonella was varied. The percentage of positive samples varied from zero to 40% in fresh turkey meat and higher than 5% in RTE turkey meat products in EU countries, the US and the Canada [5, 10–14]. The results of these studies showed that serotype profiles of the isolates were different.…”

Section: Introductionmentioning

confidence: 97%

Serotype Distribution ofSalmonellaIsolates from Turkey Ground Meat and Meat Parts

Erol

Göncüoğlu

Ayaz

et al. 2013

BioMed Research International

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The aim of the study was to find out the serotype distribution of 169 Salmonella colonies recovered from 112 Salmonella positive ground turkey (115 colonies) and 52 turkey meat parts (54 colonies). Out of 15 Salmonella serotypes: S. Corvallis, S. Kentucky, S. Bredeney, S. Virchow, S. Saintpaul and S. Agona were identified as the predominant serovars at the rates of 27%, 13%, 12%, 12%, 11%, and 10%, respectively. Other serotypes were below 6% of the total isolates. All S. Kentucky and S. Virchow and most of the S. Corvallis (39/46) and S. Heidelberg (9/9) serotypes were recovered from ground turkey. The results indicate that turkey ground meat and meat parts were contaminated with quite distinct Salmonella serotypes. This is the first study reporting Salmonella serotype distribution in turkey meat and S. Corvallis as predominant serotype in poultry meat in Turkey.

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“…DNA macrorestriction fragments were resolved on 1% SeaKem Gold agarose (Cambrex Bio Science Inc., Rockland, ME) in 0.5ϫ Tris-borate-EDTA. PFGE was carried out as previously described (11,53). Images were captured using a UV imager (Alpha Innotech, CA) and stored as .tif files.…”

Section: Methodsmentioning

confidence: 99%

Sequence Analysis and Characterization of a Transferable Hybrid Plasmid Encoding Multidrug Resistance and Enabling Zoonotic Potential for Extraintestinal Escherichia coli

et al. 2010

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ColV plasmids of extraintestinal pathogenic Escherichia coli (ExPEC) encode a variety of fitness and virulence factors and have long been associated with septicemia and avian colibacillosis. These plasmids are found significantly more often in ExPEC, including ExPEC associated with human neonatal meningitis and avian colibacillosis, than in commensal E. coli. Here we describe pAPEC-O103-ColBM, a hybrid RepFIIA/FIB plasmid harboring components of the ColV pathogenicity island and a multidrug resistance (MDR)-encoding island. This plasmid is mobilizable and confers the ability to cause septicemia in chickens, the ability to cause bacteremia resulting in meningitis in the rat model of human disease, and the ability to resist the killing effects of multiple antimicrobial agents and human serum. The results of a sequence analysis of this and other ColV plasmids supported previous findings which indicated that these plasmid types arose from a RepFIIA/FIB plasmid backbone on multiple occasions. Comparisons of pAPEC-O103-ColBM with other sequenced ColV and ColBM plasmids indicated that there is a core repertoire of virulence genes that might contribute to the ability of some ExPEC strains to cause high-level bacteremia and meningitis in a rat model. Examination of a neonatal meningitis E. coli (NMEC) population revealed that approximately 58% of the isolates examined harbored ColV-type plasmids and that 26% of these plasmids had genetic contents similar to that of pAPEC-O103-ColBM. The linkage of the ability to confer MDR and the ability contribute to multiple forms of human and animal disease on a single plasmid presents further challenges for preventing and treating ExPEC infections.ColV plasmids have a long history in scientific literature describing their association with the virulence of extraintestinal pathogenic Escherichia coli (ExPEC) (58). The first completed ColV plasmid sequence (24) localized the genes encoding the virulence traits to a 94-kb pathogenicity-associated island (PAI). Gene prevalence studies involving the genes in this PAI confirmed the hypothesis of Waters and Crosa that there are "constant" and "variable" portions of the ColV PAI and that the constant region contains the RepFIB and aerobactin operons (58). Since those studies, several more of these plasmids have been sequenced, including a plasmid variant known as ColBM, which is closely related to ColV plasmids, containing remnants of the ColV operon and the ColV PAI in a very similar arrangement (23). ColV and ColBM plasmids have been found to occur significantly more often in ExPEC than in commensal E. coli. Furthermore, recent work suggests that some ExPEC subpathotypes, including avian pathogenic E. coli (APEC) and human neonatal meningitis E. coli (NMEC), harbor these plasmids more often than other subpathotypes (10,24,26,35,42). Interestingly, although these plasmids are very prevalent in NMEC populations, the most-studied NMEC isolates appear to lack these plasmids (31, 61). Thus, far, it has been shown that ColV and ColBM plasmids co...

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Molecular Characterization and Antibiotic Resistance Profiling ofSalmonellaIsolated from Retail Turkey Meat Products

Cited by 18 publications

References 35 publications

First study of Salmonella in meat in Romania

First study of Salmonella in meat in Romania

Serotype Distribution ofSalmonellaIsolates from Turkey Ground Meat and Meat Parts

Sequence Analysis and Characterization of a Transferable Hybrid Plasmid Encoding Multidrug Resistance and Enabling Zoonotic Potential for Extraintestinal Escherichia coli

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