Molecular Characterization and Co-expression Analysis of the SnRK2 Gene Family in Sugarcane (Saccharum officinarum L.)

Li, Changning; Qian, Nong; Xie, Jinlan; Wang, Zeping; Qiang, Liang; Solanki, Manoj Kumar; Malviya, Mukesh Kumar; Liu, Xiaoyan; Li, Yijie; Htun, Reemon; Ji, Wei; Li, Yang–Rui

doi:10.1038/s41598-017-16152-4

Cited by 25 publications

(24 citation statements)

References 57 publications

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“…However, in agreement with our analysis in S . viridis , SnRK2 genes from subclasses I and II were upregulated in the phylogenetically related plant sugarcane ( Saccharum officinarum ) submitted to NaCl and PEG treatments 48 . In addition, subclass II SnRK2s 2.7 and 2.8 from Arabidopsis, which have high orthology with SvSnRK2.4, have been demonstrated to participate in the regulation of some drought-responsive genes involving the ABA-responsive element binding factors AREB/ABF 49 .…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of core abscisic acid (ABA) signaling components and their gene expression profile in response to abiotic stresses in Setaria viridis

Duarte

Souza

Santiago

et al. 2019

Sci Rep

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Abscisic acid (ABA) is an essential phytohormone that regulates growth, development and adaptation of plants to environmental stresses. In Arabidopsis and other higher plants, ABA signal transduction involves three core components namely PYR/PYL/RCAR ABA receptors (PYLs), type 2C protein phosphatases (PP2Cs) and class III SNF-1-related protein kinase 2 (SnRK2s). In the present study, we reported the identification and characterization of the core ABA signaling components in Setaria viridis, an emerging model plant for cereals and feedstock crops presenting C4 metabolism, leading to the identification of eight PYL (SvPYL1 to 8), twelve PP2C (SvPP2C1 to 12) and eleven SnRK2 (SvSnRK2.1 through SvSnRK2.11) genes. In order to study the expression profiles of these genes, two different S. viridis accessions (A10.1 and Ast-1) were submitted to drought, salinity and cold stresses, in addition to application of exogenous ABA. Differential gene expression profiles were observed in each treatment and plant genotype, demonstrating variations of ABA stress responses within the same species. These differential responses to stresses were also assessed by physiological measurements such as photosynthesis, stomatal conductance and transpiration rate. This study allows a detailed analysis of gene expression of the core ABA signaling components in Setaria viridis submitted to different treatments and provides suitable targets for genetic engineering of C4 plants aiming tolerance to abiotic stresses.

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Section: Discussionmentioning

confidence: 99%

Identification and characterization of core abscisic acid (ABA) signaling components and their gene expression profile in response to abiotic stresses in Setaria viridis

Duarte

Souza

Santiago

et al. 2019

Sci Rep

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“…It is expected that the signaling pathway relaying ABA stimuli is also conserved in monocot plants. Supporting this hypothesis, recent studies have shown that ABA strongly activates expression of SnRK2 counterparts in sugarcane (Li et al, 2017). Here we confirmed that three functional SnRK2 proteins—ScSAPK8, ScSAPK9, and ScSAPK10—are encoded by the sugarcane genome, further suggesting that the ABA-response pathway is conserved in both mono- and eudicotyledons.…”

Section: Discussionmentioning

confidence: 85%

“…officinarum hybrid cultivar ) using homologous protein sequences from A. thaliana and Sorghum bicolor . Based on previous studies in monocots and eudicots, these were designated ScSAPK8, ScSAPK9, and ScSAPK10 (Boudsocq et al, 2004; Kobayashi et al, 2004; Fujii et al, 2009; Fujita et al, 2009; Li et al, 2010; Cai et al, 2014; Li et al, 2017).…”

Section: Resultsmentioning

confidence: 99%

“…Just like their counterparts from Arabidopsis, these proteins have been shown to mediate plant responses to abiotic stress and ABA. In Saccharum officinarum L. (So) sugarcane, a recent study identified ten SnRK2 subfamily members, three of which (SoSAPK8/9/10) have the characteristic ABA box in their C-terminus and, accordingly, are responsive to ABA (Li et al, 2017). Despite these studies, currently, there is no structural and biochemical information on SnRK2 subfamily members from crop plants.…”

Section: Introductionmentioning

confidence: 99%

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The C-Terminal Domains SnRK2 Box and ABA Box Have a Role in Sugarcane SnRK2s Auto-Activation and Activity

et al. 2019

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Resistance to drought stress is fundamental to plant survival and development. Abscisic acid (ABA) is one of the major hormones involved in different types of abiotic and biotic stress responses. ABA intracellular signaling has been extensively explored in Arabidopsis thaliana and occurs via a phosphorylation cascade mediated by three related protein kinases, denominated SnRK2s (SNF1-related protein kinases). However, the role of ABA signaling and the biochemistry of SnRK2 in crop plants remains underexplored. Considering the importance of the ABA hormone in abiotic stress tolerance, here we investigated the regulatory mechanism of sugarcane SnRK2s—known as stress/ABA-activated protein kinases (SAPKs). The crystal structure of ScSAPK10 revealed the characteristic SnRK2 family architecture, in which the regulatory SnRK2 box interacts with the kinase domain αC helix. To study sugarcane SnRK2 regulation, we produced a series of mutants for the protein regulatory domains SnRK2 box and ABA box. Mutations in ScSAPK8 SnRK2 box aimed at perturbing its interaction with the protein kinase domain reduced protein kinase activity in vitro. On the other hand, mutations to ScSAPK ABA box did not impact protein kinase activity but did alter the protein autophosphorylation pattern. Taken together, our results demonstrate that both SnRK2 and ABA boxes might play a role in sugarcane SnRK2 function.

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“…Although they obtained substantial results considering the limited resources available at the time, these authors reported a relatively low number of PKs (510) in sugarcane. Other studies have indicated that sugarcane PKs are involved in this plant's development and response to environmental stimuli, such as salt, cold and drought stresses (Carraro et al, 2001;Pagariya et al, 2012;Li et al, 2017). Even more relevant is the compelling evidence that a leucine-rich repeat (LRR) receptor-like kinase is related to sucrose-accumulating sugarcane tissues and genotypes, indicating its involvement in the regulation of sucrose synthesis in mature leaves of this sugar crop (Vicentini et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

The sugarcane and sorghum kinomes: insights into evolutionary expansion and diversification

Aono

Pimenta

Garcia

et al. 2020

Preprint

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The protein kinase (PK) superfamily is one of the largest superfamilies in plants and is the core regulator of cellular signaling. Even considering this substantial importance, the kinomes of sugarcane and sorghum have not been profiled. Here we identified and profiled the complete kinomes of the polyploid Saccharum spontaneum (Ssp) and Sorghum bicolor (Sbi), a close diploid relative. The Sbi kinome was composed of 1,210 PKs; for Ssp, we identified 2,919 PKs when disregarding duplications and allelic copies, which were related to 1,345 representative gene models. The Ssp and Sbi PKs were grouped into 20 groups and 120 subfamilies and exhibited high compositional similarities and evolutionary divergences. By utilizing the collinearity between these species, this study offers insights about Sbi and Ssp speciation, PK differentiation and selection. We assessed the PK subfamily expression profiles via RNA-Seq, identifying significant similarities between Sbi and Ssp. Moreover, through coexpression networks, we inferred a core structure of kinase interactions with specific key elements. This study is the first to categorize the allele specificity of a kinome and provides a wide reservoir of molecular and genetic information, enhancing the understanding of the evolutionary history of Sbi and Ssp PKs.HighlightThis study describes the catalog of kinase gene family in Saccharum spontaneum and Sorghum bicolor, providing a reservoir of molecular features and expression patterns based on RNA-Seq and co-expression networks.

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Molecular Characterization and Co-expression Analysis of the SnRK2 Gene Family in Sugarcane (Saccharum officinarum L.)

Cited by 25 publications

References 57 publications

Identification and characterization of core abscisic acid (ABA) signaling components and their gene expression profile in response to abiotic stresses in Setaria viridis

Identification and characterization of core abscisic acid (ABA) signaling components and their gene expression profile in response to abiotic stresses in Setaria viridis

The C-Terminal Domains SnRK2 Box and ABA Box Have a Role in Sugarcane SnRK2s Auto-Activation and Activity

The sugarcane and sorghum kinomes: insights into evolutionary expansion and diversification

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