Molecular characterization and expression analysis of a complement component 3 in the sea cucumber (Apostichopus japonicus)

Zhou, Zunchun; Sun, Dan; Yang, Aifu; Dong, Ying; Zhong, Chen; Wang, Xiaoyu; Guan, Xiaoyan; Jiang, Bei; Bai, Weiliang

doi:10.1016/j.fsi.2011.06.023

Cited by 65 publications

(33 citation statements)

References 42 publications

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“…Furthermore, it was reported that LPS and dsRNA could effectively increase phagocytes and phagocytic activities in coelomocytes of the sea cucumber Holothuria glaberrima (Ramírez-Gómez et al, 2010). In this study, we found that the AjBf gene expression increased significantly after LPS challenge, which was consistent with gene C3 as we reported early (Zhou et al, 2011). Thus both Bf and C3 may be involved in the immune response against the bacteria pathogen by alternative pathway.…”

Section: Discussionsupporting

confidence: 88%

“…Its expression profiles in normal tissues and after LPS and PolyI:C challenges were also analyzed. Complement component C3, a key specific protease in the alternative pathway, had been proved to be present in the sea cucumber (Zhou et al, 2011). The isolation of Bf and C3 provided a strong evidence for the presence of an alternative activation pathway in sea cucumber.…”

Section: Discussionmentioning

confidence: 96%

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Characterization and expression analysis of a complement component gene in sea cucumber (Apostichopus japonicus)

Zhong

Zhou

Yang

et al. 2015

J. Ocean Univ. China

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The complement system plays a crucial role in the innate immune system of animals. It can be activated by distinct yet overlapping classical, alternative and lectin pathways. In the alternative pathway, complement factor B (Bf) serves as the catalytic subunit of complement component 3 (C3) convertase, which plays the central role among three activation pathways. In this study, the Bf gene in sea cucumber (Apostichopus japonicus), termed AjBf, was obtained by rapid amplification of cDNA ends (RACE). The full-length cDNA of AjBf was 3231 bp in length barring the poly (A) tail. It contained an open reading frame (ORF) of 2742 bp encoding 913 amino acids, a 105 bp 5'-UTR (5'-terminal untranslated region) and a 384 bp 3'-UTR. AjBf was a mosaic protein with six CCP (complement control protein) domains, a VWA (von Willebrand factor A) domain, and a serine protease domain. The deduced molecular weight of AjBf protein was 101 kDa. Quantitative real time PCR (qRT-PCR) analysis indicated that the expression level of AjBf in A. japonicus was obviously higher at larval stage than that at embryonic stage. Expression detection in different tissues showed that AjBf expressed higher in coelomocytes than in other four tissues. In addation, AjBf expression in different tissues was induced significantly after LPS or PolyI:C challenge. These results indicated that AjBf plays an important role in immune responses to pathogen infection.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 96%

Characterization and expression analysis of a complement component gene in sea cucumber (Apostichopus japonicus)

Zhong

Zhou

Yang

et al. 2015

J. Ocean Univ. China

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“…This is an important observation about A. japonicus complement, and may provide a theoretical basis for the development of new immunostimulants [47,48]. Zhou et al [49] studied the molecular characterization and expression analysis of C3 in the sea cucumber (A. japonicus), it was suggested that AjC3-2 and AjC3 genes play a pivotal role in immune responses to bacterial infection in sea cucumbers. The results showed that complement of A. japonicus played an important role in the immune system.…”

Section: Immune Responsementioning

confidence: 99%

Synergy of microcapsule polysaccharides and Bacillus subtilis on the growth, immunity and resistance of sea cucumber Apostichopus japonicus against Vibrio splendidus infection

Fan

et al. 2013

Fish Sci

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A 4-week feeding trial was conducted to determine the effects of different dietary supplements on the growth, immunity and resistance of sea cucumber Apostichopus japonicus against Vibrio splendidus infection. The control group was supplied with blank microcapsules, and Astragalus polysaccharide (APS) microcapsules, tuckahoe polysaccharide (TPS) microcapsules, (APS ? TPS) microcapsules, (APS ? TPS) microcapsules ? Bacillus subtilis, were tested for effects. Coelomic fluid was collected at 7-day intervals to test activities of lysozyme (LSZ), superoxide dismutase (SOD), alkaline phosphatase (AKP), and complement 3 (C3) content. After the feeding trial, the specific growth rate of sea cucumbers fed a diet supplemented with (APS ? TPS) microcapsules ? B. subtilis was significantly increased (P \ 0.05); activities of LSZ, SOD, AKP and C3 content were significantly higher than in other groups (P \ 0.05). The challenge test showed that the cumulative mortality of sea cucumbers fed a diet supplemented with (APS ? TPS) microcapsules ? B. subtilis reduced significantly (P \ 0.05). In conclusion, dietary combinations of (APS ? TPS) microcapsules ? B. subtilis has a potential for use in diet formulations for sea cucumbers to significantly increase growth, immunity and disease resistance against V. splendidus infection.

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“…As an echinoderm, sea cucumber is only endowed with innate immune system for fighting pathogens. Two isoforms of C3 genes, namely AjC3-2 and AjC3, have been found in sea cucumber (A. japonicus) by expressed sequence tags (ESTs) analysis [66]. We also found homologous proteins, namely was also found to be down-regulated in albinos.…”

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 67%

Characterization and comparison of proteomes of albino sea cucumber Apostichopus japonicus (Selenka) by iTRAQ analysis

Xia

Zhang

et al. 2016

Fish & Shellfish Immunology

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Molecular characterization and expression analysis of a complement component 3 in the sea cucumber (Apostichopus japonicus)

Cited by 65 publications

References 42 publications

Characterization and expression analysis of a complement component gene in sea cucumber (Apostichopus japonicus)

Characterization and expression analysis of a complement component gene in sea cucumber (Apostichopus japonicus)

Synergy of microcapsule polysaccharides and Bacillus subtilis on the growth, immunity and resistance of sea cucumber Apostichopus japonicus against Vibrio splendidus infection

Characterization and comparison of proteomes of albino sea cucumber Apostichopus japonicus (Selenka) by iTRAQ analysis

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